Screening and Selection of a New Medium for Diosgenin Production via Microbial Biocatalysis of <i>Fusarium</i> sp.
Steroidal saponins are widely used as starting precursors and medical intermediates for the semi-/total-synthesis of hundreds of steroidal drugs. One such steroidal saponin is diosgenin, which has attracted significant attention due to the huge market demand in the pharmaceutical industry. Due to wa...
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| Format: | Article |
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MDPI AG
2021-04-01
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| Series: | Pharmaceuticals |
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| Online Access: | https://www.mdpi.com/1424-8247/14/5/390 |
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| author | Wancang Liu Haibo Xiang Tao Zhang Xu Pang Jing Su Hongyu Liu Baiping Ma Liyan Yu |
| author_facet | Wancang Liu Haibo Xiang Tao Zhang Xu Pang Jing Su Hongyu Liu Baiping Ma Liyan Yu |
| author_sort | Wancang Liu |
| collection | DOAJ |
| description | Steroidal saponins are widely used as starting precursors and medical intermediates for the semi-/total-synthesis of hundreds of steroidal drugs. One such steroidal saponin is diosgenin, which has attracted significant attention due to the huge market demand in the pharmaceutical industry. Due to water waste and severe environmental pollution, the traditional diosgenin production process based on direct acid hydrolysis is no longer used. In this study, to develop a submerged fermentation (SmF) medium for clean diosgenin production via efficient microbial biocatalysis, the Box–Behnken design (BBD) in combination with the Plackett–Burman design (PBD) was used to determine the medium compositions for <i>Fusarium</i> strains. Three components (wheat bran, phosphate, and Tween-80) were determined as significant factors by the PBD. Using the BBD, the three significant factors were further optimized, and the optimum values were determined for maximal diosgenin production. With 21.16 g/L of wheat bran, 9.60 g/L of phosphate, and 1.97 g/L of Tween-80, the diosgenin yield was 2.28%, i.e., 3.17 mg/L/h. The experimental values agreed with the predicted values, representing a significant increase in diosgenin production compared to its production using the basic SmF medium. For the first time, we reported the development of a new medium for <i>Fusarium</i> strains to produce diosgenin via microbial biocatalysis of the root of <i>Dioscorea zingiberensis</i> C. H. Wright (DZW). A simple-composition, low-cost, and high-efficiency medium was developed for the first time for the SmF of <i>Fusarium</i> strains. The medium is considered useful for large-scale SmF and may be applicable to other fungi. This study lays a solid foundation for diosgenin production in an acid-free and wastewater-free way. It may also provide fundamental support for producing other value-added products via microbial biocatalysis of low-value materials by endophytic fungi. |
| first_indexed | 2024-03-10T12:08:05Z |
| format | Article |
| id | doaj.art-e07251b7eaf6444d95cd6235604e06f5 |
| institution | Directory Open Access Journal |
| issn | 1424-8247 |
| language | English |
| last_indexed | 2024-03-10T12:08:05Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
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| series | Pharmaceuticals |
| spelling | doaj.art-e07251b7eaf6444d95cd6235604e06f52023-11-21T16:27:19ZengMDPI AGPharmaceuticals1424-82472021-04-0114539010.3390/ph14050390Screening and Selection of a New Medium for Diosgenin Production via Microbial Biocatalysis of <i>Fusarium</i> sp.Wancang Liu0Haibo Xiang1Tao Zhang2Xu Pang3Jing Su4Hongyu Liu5Baiping Ma6Liyan Yu7Division for Medicinal Microorganisms Related Strains, CAMS Collection Center of Pathogenic Microorganisms, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, ChinaState Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan 430011, ChinaDivision for Medicinal Microorganisms Related Strains, CAMS Collection Center of Pathogenic Microorganisms, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, ChinaDivision for Medicinal Microorganisms Related Strains, CAMS Collection Center of Pathogenic Microorganisms, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, ChinaDivision for Medicinal Microorganisms Related Strains, CAMS Collection Center of Pathogenic Microorganisms, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, ChinaDivision for Medicinal Microorganisms Related Strains, CAMS Collection Center of Pathogenic Microorganisms, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, ChinaBeijing Institute of Radiation Medicine, Beijing 100850, ChinaDivision for Medicinal Microorganisms Related Strains, CAMS Collection Center of Pathogenic Microorganisms, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, ChinaSteroidal saponins are widely used as starting precursors and medical intermediates for the semi-/total-synthesis of hundreds of steroidal drugs. One such steroidal saponin is diosgenin, which has attracted significant attention due to the huge market demand in the pharmaceutical industry. Due to water waste and severe environmental pollution, the traditional diosgenin production process based on direct acid hydrolysis is no longer used. In this study, to develop a submerged fermentation (SmF) medium for clean diosgenin production via efficient microbial biocatalysis, the Box–Behnken design (BBD) in combination with the Plackett–Burman design (PBD) was used to determine the medium compositions for <i>Fusarium</i> strains. Three components (wheat bran, phosphate, and Tween-80) were determined as significant factors by the PBD. Using the BBD, the three significant factors were further optimized, and the optimum values were determined for maximal diosgenin production. With 21.16 g/L of wheat bran, 9.60 g/L of phosphate, and 1.97 g/L of Tween-80, the diosgenin yield was 2.28%, i.e., 3.17 mg/L/h. The experimental values agreed with the predicted values, representing a significant increase in diosgenin production compared to its production using the basic SmF medium. For the first time, we reported the development of a new medium for <i>Fusarium</i> strains to produce diosgenin via microbial biocatalysis of the root of <i>Dioscorea zingiberensis</i> C. H. Wright (DZW). A simple-composition, low-cost, and high-efficiency medium was developed for the first time for the SmF of <i>Fusarium</i> strains. The medium is considered useful for large-scale SmF and may be applicable to other fungi. This study lays a solid foundation for diosgenin production in an acid-free and wastewater-free way. It may also provide fundamental support for producing other value-added products via microbial biocatalysis of low-value materials by endophytic fungi.https://www.mdpi.com/1424-8247/14/5/390diosgeninmicrobial biocatalysismedium compositions<i>Dioscorea zingiberensis</i> C. H. Wrightendophytic fungus<i>Fusarium</i> strain |
| spellingShingle | Wancang Liu Haibo Xiang Tao Zhang Xu Pang Jing Su Hongyu Liu Baiping Ma Liyan Yu Screening and Selection of a New Medium for Diosgenin Production via Microbial Biocatalysis of <i>Fusarium</i> sp. Pharmaceuticals diosgenin microbial biocatalysis medium compositions <i>Dioscorea zingiberensis</i> C. H. Wright endophytic fungus <i>Fusarium</i> strain |
| title | Screening and Selection of a New Medium for Diosgenin Production via Microbial Biocatalysis of <i>Fusarium</i> sp. |
| title_full | Screening and Selection of a New Medium for Diosgenin Production via Microbial Biocatalysis of <i>Fusarium</i> sp. |
| title_fullStr | Screening and Selection of a New Medium for Diosgenin Production via Microbial Biocatalysis of <i>Fusarium</i> sp. |
| title_full_unstemmed | Screening and Selection of a New Medium for Diosgenin Production via Microbial Biocatalysis of <i>Fusarium</i> sp. |
| title_short | Screening and Selection of a New Medium for Diosgenin Production via Microbial Biocatalysis of <i>Fusarium</i> sp. |
| title_sort | screening and selection of a new medium for diosgenin production via microbial biocatalysis of i fusarium i sp |
| topic | diosgenin microbial biocatalysis medium compositions <i>Dioscorea zingiberensis</i> C. H. Wright endophytic fungus <i>Fusarium</i> strain |
| url | https://www.mdpi.com/1424-8247/14/5/390 |
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