Research on photosynthetic bacteria strain to biological formation of 5-aminolevulinic acid(光合细菌产生5-氨基乙酰丙酸(ALA)的研究)
从36个光合细菌菌株中筛选出的7株紫色非硫红假单胞菌有产ALA能力,其中菌株99-28 ALA产量最高.用紫外线对菌株99-28进行诱变处理,筛选出产量比野生菌株高1倍多的菌株L-1.对影响菌株的生长和ALA产量的因子进行了探索.抑制剂加入时间和加入次数对ALA产量有显著影响.在最佳条件下(pH 7.5,培养对数生长期加入ALA脱水酶抑制剂乙酰丙酸30 mmol,ALA生物合成前体甘氨酸30 mmol和琥珀酸30 mmol,3 000 lx光照),菌株L-1的ALA产量可达22.15 mg/L....
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| Format: | Article |
| Language: | zho |
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Zhejiang University Press
2002-05-01
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| Series: | Zhejiang Daxue xuebao. Lixue ban |
| Subjects: | |
| Online Access: | https://doi.org/zjup/1008-9497.2002.29.3.336-340 |
| _version_ | 1797236528270802944 |
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| author | LIUXiu-yang(刘秀艳) XUXiang-yang(徐向阳) CHENWei-qing(陈蔚青) |
| author_facet | LIUXiu-yang(刘秀艳) XUXiang-yang(徐向阳) CHENWei-qing(陈蔚青) |
| author_sort | LIUXiu-yang(刘秀艳) |
| collection | DOAJ |
| description | 从36个光合细菌菌株中筛选出的7株紫色非硫红假单胞菌有产ALA能力,其中菌株99-28 ALA产量最高.用紫外线对菌株99-28进行诱变处理,筛选出产量比野生菌株高1倍多的菌株L-1.对影响菌株的生长和ALA产量的因子进行了探索.抑制剂加入时间和加入次数对ALA产量有显著影响.在最佳条件下(pH 7.5,培养对数生长期加入ALA脱水酶抑制剂乙酰丙酸30 mmol,ALA生物合成前体甘氨酸30 mmol和琥珀酸30 mmol,3 000 lx光照),菌株L-1的ALA产量可达22.15 mg/L. |
| first_indexed | 2024-04-24T17:05:17Z |
| format | Article |
| id | doaj.art-e0728658cbca485196051747f725962c |
| institution | Directory Open Access Journal |
| issn | 1008-9497 |
| language | zho |
| last_indexed | 2024-04-24T17:05:17Z |
| publishDate | 2002-05-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | Zhejiang Daxue xuebao. Lixue ban |
| spelling | doaj.art-e0728658cbca485196051747f725962c2024-03-29T01:58:18ZzhoZhejiang University PressZhejiang Daxue xuebao. Lixue ban1008-94972002-05-01293336340zjup/1008-9497.2002.29.3.336-340Research on photosynthetic bacteria strain to biological formation of 5-aminolevulinic acid(光合细菌产生5-氨基乙酰丙酸(ALA)的研究)LIUXiu-yang(刘秀艳)0XUXiang-yang(徐向阳)1CHENWei-qing(陈蔚青)2 1.Department of Environmental Engineering, Zhejiang University, Hangzhou 310029, China( 1.浙江大学环境工程系，浙江 杭州 310029) 2.Hangzhou Electronic Industry University, Hangzhou 310006, China( 2.杭州电子工学院，浙江 杭州 310006) 3.Zhejiang Shuren University, Hangzhou 310006, China( 3.浙江树人大学，浙江 杭州 310006)从36个光合细菌菌株中筛选出的7株紫色非硫红假单胞菌有产ALA能力,其中菌株99-28 ALA产量最高.用紫外线对菌株99-28进行诱变处理,筛选出产量比野生菌株高1倍多的菌株L-1.对影响菌株的生长和ALA产量的因子进行了探索.抑制剂加入时间和加入次数对ALA产量有显著影响.在最佳条件下(pH 7.5,培养对数生长期加入ALA脱水酶抑制剂乙酰丙酸30 mmol,ALA生物合成前体甘氨酸30 mmol和琥珀酸30 mmol,3 000 lx光照),菌株L-1的ALA产量可达22.15 mg/L.https://doi.org/zjup/1008-9497.2002.29.3.336-340ala光合细菌乙酰丙酸甘氨酸琥珀酸 |
| spellingShingle | LIUXiu-yang(刘秀艳) XUXiang-yang(徐向阳) CHENWei-qing(陈蔚青) Research on photosynthetic bacteria strain to biological formation of 5-aminolevulinic acid(光合细菌产生5-氨基乙酰丙酸(ALA)的研究) Zhejiang Daxue xuebao. Lixue ban ala 光合细菌 乙酰丙酸 甘氨酸 琥珀酸 |
| title | Research on photosynthetic bacteria strain to biological formation of 5-aminolevulinic acid(光合细菌产生5-氨基乙酰丙酸(ALA)的研究) |
| title_full | Research on photosynthetic bacteria strain to biological formation of 5-aminolevulinic acid(光合细菌产生5-氨基乙酰丙酸(ALA)的研究) |
| title_fullStr | Research on photosynthetic bacteria strain to biological formation of 5-aminolevulinic acid(光合细菌产生5-氨基乙酰丙酸(ALA)的研究) |
| title_full_unstemmed | Research on photosynthetic bacteria strain to biological formation of 5-aminolevulinic acid(光合细菌产生5-氨基乙酰丙酸(ALA)的研究) |
| title_short | Research on photosynthetic bacteria strain to biological formation of 5-aminolevulinic acid(光合细菌产生5-氨基乙酰丙酸(ALA)的研究) |
| title_sort | research on photosynthetic bacteria strain to biological formation of 5 aminolevulinic acid 光合细菌产生5 氨基乙酰丙酸 ala 的研究 |
| topic | ala 光合细菌 乙酰丙酸 甘氨酸 琥珀酸 |
| url | https://doi.org/zjup/1008-9497.2002.29.3.336-340 |
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