MiRNA Expression Profile in the Airways Is Altered during Pulmonary Exacerbation in Children with Cystic Fibrosis—A Preliminary Report
MicroRNAs are small non-coding RNAs that regulate immune response and inflammation. We assumed that miRNAs may be involved in the immune response during cystic fibrosis pulmonary exacerbations (CFPE) and that altered expression profile in the airways and blood may underlie clinical outcomes in CF pe...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-06-01
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| Series: | Journal of Clinical Medicine |
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| Online Access: | https://www.mdpi.com/2077-0383/9/6/1887 |
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| author | Zuzanna Stachowiak Irena Wojsyk-Banaszak Katarzyna Jończyk-Potoczna Beata Narożna Wojciech Langwiński Zdzisława Kycler Paulina Sobkowiak Anna Bręborowicz Aleksandra Szczepankiewicz |
| author_facet | Zuzanna Stachowiak Irena Wojsyk-Banaszak Katarzyna Jończyk-Potoczna Beata Narożna Wojciech Langwiński Zdzisława Kycler Paulina Sobkowiak Anna Bręborowicz Aleksandra Szczepankiewicz |
| author_sort | Zuzanna Stachowiak |
| collection | DOAJ |
| description | MicroRNAs are small non-coding RNAs that regulate immune response and inflammation. We assumed that miRNAs may be involved in the immune response during cystic fibrosis pulmonary exacerbations (CFPE) and that altered expression profile in the airways and blood may underlie clinical outcomes in CF pediatric patients. Methods: We included 30 pediatric patients diagnosed with cystic fibrosis. The biologic material (blood, sputum, exhaled breath condensate) was collected during pulmonary exacerbation and in stable condition. The miRNA expression profile from blood and sputum (<i>n</i> = 6) was done using the next-generation sequencing. For validation, selected four miRNAs were analyzed by qPCR in exosomes from sputum supernatant and exhaled breath condensate (<i>n</i> = 24). NGS analysis was done in Base Space, correlations of gene expression with clinical data were done in Statistica. Results: The miRNA profiling showed that four miRNAs (miR-223, miR-451a, miR-27b-3p, miR-486-5p) were significantly altered during pulmonary exacerbation in CF patients in sputum but did not differ significantly in blood. MiRNA differently expressed in exhaled breath condensate (EBC) and sputum showed correlation with clinical parameters in CFPE. Conclusion: MiRNA expression profile changes in the airways during pulmonary exacerbation in CF pediatric patients. We suggest that miRNA alterations during CFPE are restricted to the airways and strongly correlate with clinical outcome. |
| first_indexed | 2024-03-10T19:07:30Z |
| format | Article |
| id | doaj.art-e0768660abfc4a8db334965b9753b870 |
| institution | Directory Open Access Journal |
| issn | 2077-0383 |
| language | English |
| last_indexed | 2024-03-10T19:07:30Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Clinical Medicine |
| spelling | doaj.art-e0768660abfc4a8db334965b9753b8702023-11-20T04:03:07ZengMDPI AGJournal of Clinical Medicine2077-03832020-06-0196188710.3390/jcm9061887MiRNA Expression Profile in the Airways Is Altered during Pulmonary Exacerbation in Children with Cystic Fibrosis—A Preliminary ReportZuzanna Stachowiak0Irena Wojsyk-Banaszak1Katarzyna Jończyk-Potoczna2Beata Narożna3Wojciech Langwiński4Zdzisława Kycler5Paulina Sobkowiak6Anna Bręborowicz7Aleksandra Szczepankiewicz8Molecular and Cell Biology Unit, Department of Paediatric Pulmonology, Allergy and Clinical Immunology, Poznan University of Medical Sciences, 60-572 Poznań, PolandDepartment of Paediatric Pulmonology, Allergy and Clinical Immunology, Poznan University of Medical Sciences, 60-572 Poznań, PolandDepartment of Paediatric Radiology, Poznan University of Medical Sciences, 60-572 Poznań, PolandMolecular and Cell Biology Unit, Department of Paediatric Pulmonology, Allergy and Clinical Immunology, Poznan University of Medical Sciences, 60-572 Poznań, PolandMolecular and Cell Biology Unit, Department of Paediatric Pulmonology, Allergy and Clinical Immunology, Poznan University of Medical Sciences, 60-572 Poznań, PolandDepartment of Paediatric Pulmonology, Allergy and Clinical Immunology, Poznan University of Medical Sciences, 60-572 Poznań, PolandDepartment of Paediatric Pulmonology, Allergy and Clinical Immunology, Poznan University of Medical Sciences, 60-572 Poznań, PolandDepartment of Paediatric Pulmonology, Allergy and Clinical Immunology, Poznan University of Medical Sciences, 60-572 Poznań, PolandMolecular and Cell Biology Unit, Department of Paediatric Pulmonology, Allergy and Clinical Immunology, Poznan University of Medical Sciences, 60-572 Poznań, PolandMicroRNAs are small non-coding RNAs that regulate immune response and inflammation. We assumed that miRNAs may be involved in the immune response during cystic fibrosis pulmonary exacerbations (CFPE) and that altered expression profile in the airways and blood may underlie clinical outcomes in CF pediatric patients. Methods: We included 30 pediatric patients diagnosed with cystic fibrosis. The biologic material (blood, sputum, exhaled breath condensate) was collected during pulmonary exacerbation and in stable condition. The miRNA expression profile from blood and sputum (<i>n</i> = 6) was done using the next-generation sequencing. For validation, selected four miRNAs were analyzed by qPCR in exosomes from sputum supernatant and exhaled breath condensate (<i>n</i> = 24). NGS analysis was done in Base Space, correlations of gene expression with clinical data were done in Statistica. Results: The miRNA profiling showed that four miRNAs (miR-223, miR-451a, miR-27b-3p, miR-486-5p) were significantly altered during pulmonary exacerbation in CF patients in sputum but did not differ significantly in blood. MiRNA differently expressed in exhaled breath condensate (EBC) and sputum showed correlation with clinical parameters in CFPE. Conclusion: MiRNA expression profile changes in the airways during pulmonary exacerbation in CF pediatric patients. We suggest that miRNA alterations during CFPE are restricted to the airways and strongly correlate with clinical outcome.https://www.mdpi.com/2077-0383/9/6/1887miRNA expressionexhaled breath condensatesputumseveritypulmonary exacerbation |
| spellingShingle | Zuzanna Stachowiak Irena Wojsyk-Banaszak Katarzyna Jończyk-Potoczna Beata Narożna Wojciech Langwiński Zdzisława Kycler Paulina Sobkowiak Anna Bręborowicz Aleksandra Szczepankiewicz MiRNA Expression Profile in the Airways Is Altered during Pulmonary Exacerbation in Children with Cystic Fibrosis—A Preliminary Report Journal of Clinical Medicine miRNA expression exhaled breath condensate sputum severity pulmonary exacerbation |
| title | MiRNA Expression Profile in the Airways Is Altered during Pulmonary Exacerbation in Children with Cystic Fibrosis—A Preliminary Report |
| title_full | MiRNA Expression Profile in the Airways Is Altered during Pulmonary Exacerbation in Children with Cystic Fibrosis—A Preliminary Report |
| title_fullStr | MiRNA Expression Profile in the Airways Is Altered during Pulmonary Exacerbation in Children with Cystic Fibrosis—A Preliminary Report |
| title_full_unstemmed | MiRNA Expression Profile in the Airways Is Altered during Pulmonary Exacerbation in Children with Cystic Fibrosis—A Preliminary Report |
| title_short | MiRNA Expression Profile in the Airways Is Altered during Pulmonary Exacerbation in Children with Cystic Fibrosis—A Preliminary Report |
| title_sort | mirna expression profile in the airways is altered during pulmonary exacerbation in children with cystic fibrosis a preliminary report |
| topic | miRNA expression exhaled breath condensate sputum severity pulmonary exacerbation |
| url | https://www.mdpi.com/2077-0383/9/6/1887 |
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