Cytosine base editing systems with minimized off-target effect and molecular size
Base editing is promising for gene therapy, but in vivo delivery has been limiting. Here the authors perform structure-based rational engineering of the cytosine base editing system Target-AID to minimise off-target effects and decrease its size.
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-08-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-022-32157-8 |
| _version_ | 1811215397011062784 |
|---|---|
| author | Ang Li Hitoshi Mitsunobu Shin Yoshioka Takahisa Suzuki Akihiko Kondo Keiji Nishida |
| author_facet | Ang Li Hitoshi Mitsunobu Shin Yoshioka Takahisa Suzuki Akihiko Kondo Keiji Nishida |
| author_sort | Ang Li |
| collection | DOAJ |
| description | Base editing is promising for gene therapy, but in vivo delivery has been limiting. Here the authors perform structure-based rational engineering of the cytosine base editing system Target-AID to minimise off-target effects and decrease its size. |
| first_indexed | 2024-04-12T06:21:59Z |
| format | Article |
| id | doaj.art-e076e676da444a21bde4bde19c94cc86 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-04-12T06:21:59Z |
| publishDate | 2022-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-e076e676da444a21bde4bde19c94cc862022-12-22T03:44:17ZengNature PortfolioNature Communications2041-17232022-08-011311810.1038/s41467-022-32157-8Cytosine base editing systems with minimized off-target effect and molecular sizeAng Li0Hitoshi Mitsunobu1Shin Yoshioka2Takahisa Suzuki3Akihiko Kondo4Keiji Nishida5Graduate School of Science, Technology and Innovation, Kobe UniversityEngineering Biology Research Center, Kobe UniversityGraduate School of Science, Technology and Innovation, Kobe UniversityGraduate School of Science, Technology and Innovation, Kobe UniversityGraduate School of Science, Technology and Innovation, Kobe UniversityGraduate School of Science, Technology and Innovation, Kobe UniversityBase editing is promising for gene therapy, but in vivo delivery has been limiting. Here the authors perform structure-based rational engineering of the cytosine base editing system Target-AID to minimise off-target effects and decrease its size.https://doi.org/10.1038/s41467-022-32157-8 |
| spellingShingle | Ang Li Hitoshi Mitsunobu Shin Yoshioka Takahisa Suzuki Akihiko Kondo Keiji Nishida Cytosine base editing systems with minimized off-target effect and molecular size Nature Communications |
| title | Cytosine base editing systems with minimized off-target effect and molecular size |
| title_full | Cytosine base editing systems with minimized off-target effect and molecular size |
| title_fullStr | Cytosine base editing systems with minimized off-target effect and molecular size |
| title_full_unstemmed | Cytosine base editing systems with minimized off-target effect and molecular size |
| title_short | Cytosine base editing systems with minimized off-target effect and molecular size |
| title_sort | cytosine base editing systems with minimized off target effect and molecular size |
| url | https://doi.org/10.1038/s41467-022-32157-8 |
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