Quantitative trait loci for Fusarium head blight resistance in wheat cultivars Yangmai 158 and Zhengmai 9023
Fusarium head blight (FHB) is one of the prevalent fungal diseases of wheat worldwide. Exploring new FHB resistance quantitative trait loci (QTL) in adapted wheat cultivars is a critical step for breeding new FHB-resistant cultivars. In this study, we developed a population of 236 F5:7 recombinant i...
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KeAi Communications Co., Ltd.
2021-02-01
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| Series: | Crop Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214514120300854 |
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| author | Pingping Zhang Chenjin Guo Zhao Liu Amy Bernardo Hongxiang Ma Peng Jiang Guicheng Song Guihua Bai |
| author_facet | Pingping Zhang Chenjin Guo Zhao Liu Amy Bernardo Hongxiang Ma Peng Jiang Guicheng Song Guihua Bai |
| author_sort | Pingping Zhang |
| collection | DOAJ |
| description | Fusarium head blight (FHB) is one of the prevalent fungal diseases of wheat worldwide. Exploring new FHB resistance quantitative trait loci (QTL) in adapted wheat cultivars is a critical step for breeding new FHB-resistant cultivars. In this study, we developed a population of 236 F5:7 recombinant inbred lines (RILs) using two popular Chinese wheat cultivars, Yangmai 158 and Zhengmai 9023, with moderate FHB resistance to identify the QTL for FHB type II resistance. This population was evaluated for percentage of symptomatic spikelets per spike (PSS) using single floret injection in repeated greenhouse experiments. Mean PSSs were 33.2% for Yangmai 158 and 30.3% for Zhengmai 9023. A genetic linkage map of 1002 single nucleotide polymorphisms (SNPs) generated by genotyping-by-sequencing (GBS) was constructed for the RIL population. Six QTL were identified for FHB resistance, and three of them were repeatable in the both experiments. Zhengmai 9023 contributed the resistance allele at one repeatable QTL, designated as Qfhb.7D, whereas Yangmai 158 contributed the resistance alleles at the other two repeatable QTL, Qfhb.3AL and Qfhb.2DS. The additional QTL, Qfhb.4AS was significant in the mean PSS, and Qfhb.2DL and Qfhb.7AS were significant in only one experiment. Replacement of each allele individually at the three repeatable QTL significantly changed PSSs. Qfhb.3AL, Qfhb.2DS, and Qfhb.7D explained 8.35% to 9.89%, 5.13% to 7.43%, and 6.15% to 9.32% of the phenotypic variations, respectively. The three repeatable QTL contributed by the two parents were additive and stacking the resistance alleles from all the three repeatable QTL showed the highest level of resistance in the current RIL population. Ten SNPs in the QTL regions of Qfhb.3AL, Qfhb.2DS, and Qfhb.7D were converted into KBioscience competitive allele-specific PCR (KASP) assays. One KASP marker for Qfhb.3AL was validated in a panel of wheat cultivars from China. Some of these KASP markers could be useful for marker-assisted selection to stack these QTL. |
| first_indexed | 2024-12-22T21:44:23Z |
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| id | doaj.art-e07ad32b0aa94d0d80fef68179927a36 |
| institution | Directory Open Access Journal |
| issn | 2214-5141 |
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| last_indexed | 2024-12-22T21:44:23Z |
| publishDate | 2021-02-01 |
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| series | Crop Journal |
| spelling | doaj.art-e07ad32b0aa94d0d80fef68179927a362022-12-21T18:11:31ZengKeAi Communications Co., Ltd.Crop Journal2214-51412021-02-0191143153Quantitative trait loci for Fusarium head blight resistance in wheat cultivars Yangmai 158 and Zhengmai 9023Pingping Zhang0Chenjin Guo1Zhao Liu2Amy Bernardo3Hongxiang Ma4Peng Jiang5Guicheng Song6Guihua Bai7Jiangsu Academy of Agricultural Sciences, Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing 210014, Jiangsu, China; Department of Agronomy, Kansas State University, Manhattan, KS 66506, USADepartment of Agronomy, Kansas State University, Manhattan, KS 66506, USA; Hebei Agricultural University, Baoding 071001, Hebei, ChinaDepartment of Agronomy, Kansas State University, Manhattan, KS 66506, USAUSDA-ARS, Hard Winter Wheat Genetics Research Unit, Manhattan, KS 66506, USAJiangsu Academy of Agricultural Sciences, Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing 210014, Jiangsu, ChinaJiangsu Academy of Agricultural Sciences, Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing 210014, Jiangsu, ChinaJiangsu Academy of Agricultural Sciences, Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing 210014, Jiangsu, ChinaDepartment of Agronomy, Kansas State University, Manhattan, KS 66506, USA; USDA-ARS, Hard Winter Wheat Genetics Research Unit, Manhattan, KS 66506, USA; Corresponding author at: Department of Agronomy, Kansas State University, Manhattan, KS 66506, USA.Fusarium head blight (FHB) is one of the prevalent fungal diseases of wheat worldwide. Exploring new FHB resistance quantitative trait loci (QTL) in adapted wheat cultivars is a critical step for breeding new FHB-resistant cultivars. In this study, we developed a population of 236 F5:7 recombinant inbred lines (RILs) using two popular Chinese wheat cultivars, Yangmai 158 and Zhengmai 9023, with moderate FHB resistance to identify the QTL for FHB type II resistance. This population was evaluated for percentage of symptomatic spikelets per spike (PSS) using single floret injection in repeated greenhouse experiments. Mean PSSs were 33.2% for Yangmai 158 and 30.3% for Zhengmai 9023. A genetic linkage map of 1002 single nucleotide polymorphisms (SNPs) generated by genotyping-by-sequencing (GBS) was constructed for the RIL population. Six QTL were identified for FHB resistance, and three of them were repeatable in the both experiments. Zhengmai 9023 contributed the resistance allele at one repeatable QTL, designated as Qfhb.7D, whereas Yangmai 158 contributed the resistance alleles at the other two repeatable QTL, Qfhb.3AL and Qfhb.2DS. The additional QTL, Qfhb.4AS was significant in the mean PSS, and Qfhb.2DL and Qfhb.7AS were significant in only one experiment. Replacement of each allele individually at the three repeatable QTL significantly changed PSSs. Qfhb.3AL, Qfhb.2DS, and Qfhb.7D explained 8.35% to 9.89%, 5.13% to 7.43%, and 6.15% to 9.32% of the phenotypic variations, respectively. The three repeatable QTL contributed by the two parents were additive and stacking the resistance alleles from all the three repeatable QTL showed the highest level of resistance in the current RIL population. Ten SNPs in the QTL regions of Qfhb.3AL, Qfhb.2DS, and Qfhb.7D were converted into KBioscience competitive allele-specific PCR (KASP) assays. One KASP marker for Qfhb.3AL was validated in a panel of wheat cultivars from China. Some of these KASP markers could be useful for marker-assisted selection to stack these QTL.http://www.sciencedirect.com/science/article/pii/S2214514120300854QTL mappingFHBYangmai 158Zhengmai 9023KASP |
| spellingShingle | Pingping Zhang Chenjin Guo Zhao Liu Amy Bernardo Hongxiang Ma Peng Jiang Guicheng Song Guihua Bai Quantitative trait loci for Fusarium head blight resistance in wheat cultivars Yangmai 158 and Zhengmai 9023 Crop Journal QTL mapping FHB Yangmai 158 Zhengmai 9023 KASP |
| title | Quantitative trait loci for Fusarium head blight resistance in wheat cultivars Yangmai 158 and Zhengmai 9023 |
| title_full | Quantitative trait loci for Fusarium head blight resistance in wheat cultivars Yangmai 158 and Zhengmai 9023 |
| title_fullStr | Quantitative trait loci for Fusarium head blight resistance in wheat cultivars Yangmai 158 and Zhengmai 9023 |
| title_full_unstemmed | Quantitative trait loci for Fusarium head blight resistance in wheat cultivars Yangmai 158 and Zhengmai 9023 |
| title_short | Quantitative trait loci for Fusarium head blight resistance in wheat cultivars Yangmai 158 and Zhengmai 9023 |
| title_sort | quantitative trait loci for fusarium head blight resistance in wheat cultivars yangmai 158 and zhengmai 9023 |
| topic | QTL mapping FHB Yangmai 158 Zhengmai 9023 KASP |
| url | http://www.sciencedirect.com/science/article/pii/S2214514120300854 |
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