A Non-thermal Biocompatible Plasma-Modified Chitosan Scaffold Enhances Osteogenic Differentiation in Bone Marrow Stem Cells
Non-thermal biocompatible plasma (NBP) was considered as an efficient tool in tissue engineering to modify the surface of biomaterials. Three-dimensional chitosan scaffolds have been extensively used in different ways because it holds some remarkable properties, including biodegradability and biocom...
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MDPI AG
2022-02-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/14/2/465 |
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| author | Ihn Han Juie Nahushkumar Rana Ji-Hye Kim Eun Ha Choi Youngsun Kim |
| author_facet | Ihn Han Juie Nahushkumar Rana Ji-Hye Kim Eun Ha Choi Youngsun Kim |
| author_sort | Ihn Han |
| collection | DOAJ |
| description | Non-thermal biocompatible plasma (NBP) was considered as an efficient tool in tissue engineering to modify the surface of biomaterials. Three-dimensional chitosan scaffolds have been extensively used in different ways because it holds some remarkable properties, including biodegradability and biocompatibility. In this study, we evaluated the osteogenic potential of NBP-treated chitosan scaffolds using two different plasma sources: a dielectric barrier discharge (NBP-DBD) and a soft jet (NBP-J). The surface modification of the scaffold was evaluated using scanning electron microscopy. For osteogenic differentiation of cells, proliferation and differentiation were tested by using bone marrow-derived stem cells (BMSCs). We observed that cell viability using NBP-DBD and NBP-J treated chitosan scaffolds yielded significant improvements in cell viability and differentiation. The results obtained with MTT and live/dead assays showed that NBP-modified scaffold increases cell metabolic by MTT assay and live/dead assay. It also observed that the NBP treatment is more effective at 5 min with DBD and was selected for further investigations. Enhanced osteogenic differentiation was observed using NBP-treated scaffolds, as reflected by increased alkaline phosphatase activity. Our findings showed that NBP is an innovative and beneficial tool for modifying chitosan scaffolds to increase their activity, making them suitable as biocompatible materials and for bone tissue engineering. |
| first_indexed | 2024-03-09T21:14:26Z |
| format | Article |
| id | doaj.art-e07ecabe343a480e8a9285f7cd36a3c5 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-09T21:14:26Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-e07ecabe343a480e8a9285f7cd36a3c52023-11-23T21:39:48ZengMDPI AGPharmaceutics1999-49232022-02-0114246510.3390/pharmaceutics14020465A Non-thermal Biocompatible Plasma-Modified Chitosan Scaffold Enhances Osteogenic Differentiation in Bone Marrow Stem CellsIhn Han0Juie Nahushkumar Rana1Ji-Hye Kim2Eun Ha Choi3Youngsun Kim4Department of Plasma Bio Display, Kwangwoon University, Seoul 01897, KoreaDepartment of Plasma Bio Display, Kwangwoon University, Seoul 01897, KoreaEllitech Medical Incorporation, Seoul 02584, KoreaPlasma Bioscience Research Center, Kwangwoon University, Seoul 01897, KoreaDepartment of Obstetrics and Gynecology, Kyung Hee University Medical Center, Seoul 02447, KoreaNon-thermal biocompatible plasma (NBP) was considered as an efficient tool in tissue engineering to modify the surface of biomaterials. Three-dimensional chitosan scaffolds have been extensively used in different ways because it holds some remarkable properties, including biodegradability and biocompatibility. In this study, we evaluated the osteogenic potential of NBP-treated chitosan scaffolds using two different plasma sources: a dielectric barrier discharge (NBP-DBD) and a soft jet (NBP-J). The surface modification of the scaffold was evaluated using scanning electron microscopy. For osteogenic differentiation of cells, proliferation and differentiation were tested by using bone marrow-derived stem cells (BMSCs). We observed that cell viability using NBP-DBD and NBP-J treated chitosan scaffolds yielded significant improvements in cell viability and differentiation. The results obtained with MTT and live/dead assays showed that NBP-modified scaffold increases cell metabolic by MTT assay and live/dead assay. It also observed that the NBP treatment is more effective at 5 min with DBD and was selected for further investigations. Enhanced osteogenic differentiation was observed using NBP-treated scaffolds, as reflected by increased alkaline phosphatase activity. Our findings showed that NBP is an innovative and beneficial tool for modifying chitosan scaffolds to increase their activity, making them suitable as biocompatible materials and for bone tissue engineering.https://www.mdpi.com/1999-4923/14/2/465non-thermal plasmabiomaterialchitosan scaffoldsosteogenic differentiationtissue engineering |
| spellingShingle | Ihn Han Juie Nahushkumar Rana Ji-Hye Kim Eun Ha Choi Youngsun Kim A Non-thermal Biocompatible Plasma-Modified Chitosan Scaffold Enhances Osteogenic Differentiation in Bone Marrow Stem Cells Pharmaceutics non-thermal plasma biomaterial chitosan scaffolds osteogenic differentiation tissue engineering |
| title | A Non-thermal Biocompatible Plasma-Modified Chitosan Scaffold Enhances Osteogenic Differentiation in Bone Marrow Stem Cells |
| title_full | A Non-thermal Biocompatible Plasma-Modified Chitosan Scaffold Enhances Osteogenic Differentiation in Bone Marrow Stem Cells |
| title_fullStr | A Non-thermal Biocompatible Plasma-Modified Chitosan Scaffold Enhances Osteogenic Differentiation in Bone Marrow Stem Cells |
| title_full_unstemmed | A Non-thermal Biocompatible Plasma-Modified Chitosan Scaffold Enhances Osteogenic Differentiation in Bone Marrow Stem Cells |
| title_short | A Non-thermal Biocompatible Plasma-Modified Chitosan Scaffold Enhances Osteogenic Differentiation in Bone Marrow Stem Cells |
| title_sort | non thermal biocompatible plasma modified chitosan scaffold enhances osteogenic differentiation in bone marrow stem cells |
| topic | non-thermal plasma biomaterial chitosan scaffolds osteogenic differentiation tissue engineering |
| url | https://www.mdpi.com/1999-4923/14/2/465 |
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