Anatomy and activity patterns in a multifunctional motor neuron and its surrounding circuits
Dorsal Excitor motor neuron DE-3 in the medicinal leech plays three very different dynamical roles in three different behaviors. Without rewiring its anatomical connectivity, how can a motor neuron dynamically switch roles to play appropriate roles in various behaviors? We previously used voltage-se...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2021-02-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/61881 |
| _version_ | 1811202668235849728 |
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| author | Mária Ashaber Yusuke Tomina Pegah Kassraian Eric A Bushong William B Kristan Mark H Ellisman Daniel A Wagenaar |
| author_facet | Mária Ashaber Yusuke Tomina Pegah Kassraian Eric A Bushong William B Kristan Mark H Ellisman Daniel A Wagenaar |
| author_sort | Mária Ashaber |
| collection | DOAJ |
| description | Dorsal Excitor motor neuron DE-3 in the medicinal leech plays three very different dynamical roles in three different behaviors. Without rewiring its anatomical connectivity, how can a motor neuron dynamically switch roles to play appropriate roles in various behaviors? We previously used voltage-sensitive dye imaging to record from DE-3 and most other neurons in the leech segmental ganglion during (fictive) swimming, crawling, and local-bend escape (Tomina and Wagenaar, 2017). Here, we repeated that experiment, then re-imaged the same ganglion using serial blockface electron microscopy and traced DE-3’s processes. Further, we traced back the processes of DE-3’s presynaptic partners to their respective somata. This allowed us to analyze the relationship between circuit anatomy and the activity patterns it sustains. We found that input synapses important for all the behaviors were widely distributed over DE-3’s branches, yet that functional clusters were different during (fictive) swimming vs. crawling. |
| first_indexed | 2024-04-12T02:42:30Z |
| format | Article |
| id | doaj.art-e07efdba9afa4d5c9a1b4010634b5517 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T02:42:30Z |
| publishDate | 2021-02-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-e07efdba9afa4d5c9a1b4010634b55172022-12-22T03:51:17ZengeLife Sciences Publications LtdeLife2050-084X2021-02-011010.7554/eLife.61881Anatomy and activity patterns in a multifunctional motor neuron and its surrounding circuitsMária Ashaber0https://orcid.org/0000-0002-5586-9585Yusuke Tomina1Pegah Kassraian2Eric A Bushong3https://orcid.org/0000-0001-6195-2433William B Kristan4Mark H Ellisman5Daniel A Wagenaar6https://orcid.org/0000-0002-6222-761XDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, United StatesDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, United StatesDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, United StatesDivision of Biological Sciences, University of California, San Diego, San Diego, United StatesDivision of Biological Sciences, University of California, San Diego, San Diego, United StatesNational Center for Microscopy and Imaging Research, University of California, San Diego, San Diego, United States; Department of Neurosciences, UCSD School of Medicine, San Diego, United StatesDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, United StatesDorsal Excitor motor neuron DE-3 in the medicinal leech plays three very different dynamical roles in three different behaviors. Without rewiring its anatomical connectivity, how can a motor neuron dynamically switch roles to play appropriate roles in various behaviors? We previously used voltage-sensitive dye imaging to record from DE-3 and most other neurons in the leech segmental ganglion during (fictive) swimming, crawling, and local-bend escape (Tomina and Wagenaar, 2017). Here, we repeated that experiment, then re-imaged the same ganglion using serial blockface electron microscopy and traced DE-3’s processes. Further, we traced back the processes of DE-3’s presynaptic partners to their respective somata. This allowed us to analyze the relationship between circuit anatomy and the activity patterns it sustains. We found that input synapses important for all the behaviors were widely distributed over DE-3’s branches, yet that functional clusters were different during (fictive) swimming vs. crawling.https://elifesciences.org/articles/61881Hirudo verbanavoltage-sensitive dye imagingserial blockface electron microscopymotor behaviorsconnectomeneural circuit |
| spellingShingle | Mária Ashaber Yusuke Tomina Pegah Kassraian Eric A Bushong William B Kristan Mark H Ellisman Daniel A Wagenaar Anatomy and activity patterns in a multifunctional motor neuron and its surrounding circuits eLife Hirudo verbana voltage-sensitive dye imaging serial blockface electron microscopy motor behaviors connectome neural circuit |
| title | Anatomy and activity patterns in a multifunctional motor neuron and its surrounding circuits |
| title_full | Anatomy and activity patterns in a multifunctional motor neuron and its surrounding circuits |
| title_fullStr | Anatomy and activity patterns in a multifunctional motor neuron and its surrounding circuits |
| title_full_unstemmed | Anatomy and activity patterns in a multifunctional motor neuron and its surrounding circuits |
| title_short | Anatomy and activity patterns in a multifunctional motor neuron and its surrounding circuits |
| title_sort | anatomy and activity patterns in a multifunctional motor neuron and its surrounding circuits |
| topic | Hirudo verbana voltage-sensitive dye imaging serial blockface electron microscopy motor behaviors connectome neural circuit |
| url | https://elifesciences.org/articles/61881 |
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