Molecular Regulation of Porcine Skeletal Muscle Development: Insights from Research on CDC23 Expression and Function
Cell division cycle 23 (CDC23) is a component of the tetratricopeptide repeat (TPR) subunit in the anaphase-promoting complex or cyclosome (APC/C) complex, which participates in the regulation of mitosis in eukaryotes. However, the regulatory model and mechanism by which the CDC23 gene regulates mus...
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| Format: | Article |
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MDPI AG
2024-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/25/7/3664 |
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| author | Su Xie Quan Liu Chong Fu Yansen Chen Mengxun Li Cheng Tian Jiaxuan Li Min Han Changchun Li |
| author_facet | Su Xie Quan Liu Chong Fu Yansen Chen Mengxun Li Cheng Tian Jiaxuan Li Min Han Changchun Li |
| author_sort | Su Xie |
| collection | DOAJ |
| description | Cell division cycle 23 (CDC23) is a component of the tetratricopeptide repeat (TPR) subunit in the anaphase-promoting complex or cyclosome (APC/C) complex, which participates in the regulation of mitosis in eukaryotes. However, the regulatory model and mechanism by which the CDC23 gene regulates muscle production in pigs are largely unknown. In this study, we investigated the expression of CDC23 in pigs, and the results indicated that CDC23 is widely expressed in various tissues and organs. In vitro cell experiments have demonstrated that CDC23 promotes the proliferation of myoblasts, as well as significantly positively regulating the differentiation of skeletal muscle satellite cells. In addition, Gene Set Enrichment Analysis (GSEA) revealed a significant downregulation of the cell cycle pathway during the differentiation process of skeletal muscle satellite cells. The protein–protein interaction (PPI) network showed a high degree of interaction between genes related to the cell cycle pathway and CDC23. Subsequently, in differentiated myocytes induced after overexpression of CDC23, the level of CDC23 exhibited a significant negative correlation with the expression of key factors in the cell cycle pathway, suggesting that CDC23 may be involved in the inhibition of the cell cycle signaling pathway in order to promote the differentiation process. In summary, we preliminarily determined the function of CDC23 with the aim of providing new insights into molecular regulation during porcine skeletal muscle development. |
| first_indexed | 2024-04-24T10:43:51Z |
| format | Article |
| id | doaj.art-e082281905cb457caa585fd08b380d08 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-04-24T10:43:51Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-e082281905cb457caa585fd08b380d082024-04-12T13:19:24ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672024-03-01257366410.3390/ijms25073664Molecular Regulation of Porcine Skeletal Muscle Development: Insights from Research on CDC23 Expression and FunctionSu Xie0Quan Liu1Chong Fu2Yansen Chen3Mengxun Li4Cheng Tian5Jiaxuan Li6Min Han7Changchun Li8Key Laboratory of Swine Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Swine Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Swine Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaTERRA Teaching and Research Center, University of Liège, Gembloux Agro-Bio Tech (ULiège-GxABT), 5030 Gembloux, BelgiumKey Laboratory of Swine Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Swine Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Swine Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Swine Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Swine Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaCell division cycle 23 (CDC23) is a component of the tetratricopeptide repeat (TPR) subunit in the anaphase-promoting complex or cyclosome (APC/C) complex, which participates in the regulation of mitosis in eukaryotes. However, the regulatory model and mechanism by which the CDC23 gene regulates muscle production in pigs are largely unknown. In this study, we investigated the expression of CDC23 in pigs, and the results indicated that CDC23 is widely expressed in various tissues and organs. In vitro cell experiments have demonstrated that CDC23 promotes the proliferation of myoblasts, as well as significantly positively regulating the differentiation of skeletal muscle satellite cells. In addition, Gene Set Enrichment Analysis (GSEA) revealed a significant downregulation of the cell cycle pathway during the differentiation process of skeletal muscle satellite cells. The protein–protein interaction (PPI) network showed a high degree of interaction between genes related to the cell cycle pathway and CDC23. Subsequently, in differentiated myocytes induced after overexpression of CDC23, the level of CDC23 exhibited a significant negative correlation with the expression of key factors in the cell cycle pathway, suggesting that CDC23 may be involved in the inhibition of the cell cycle signaling pathway in order to promote the differentiation process. In summary, we preliminarily determined the function of CDC23 with the aim of providing new insights into molecular regulation during porcine skeletal muscle development.https://www.mdpi.com/1422-0067/25/7/3664CDC23porcine satellite cells (PSCs)myoblast differentiationGSEAmyoblast proliferationcell cycle pathway |
| spellingShingle | Su Xie Quan Liu Chong Fu Yansen Chen Mengxun Li Cheng Tian Jiaxuan Li Min Han Changchun Li Molecular Regulation of Porcine Skeletal Muscle Development: Insights from Research on CDC23 Expression and Function International Journal of Molecular Sciences CDC23 porcine satellite cells (PSCs) myoblast differentiation GSEA myoblast proliferation cell cycle pathway |
| title | Molecular Regulation of Porcine Skeletal Muscle Development: Insights from Research on CDC23 Expression and Function |
| title_full | Molecular Regulation of Porcine Skeletal Muscle Development: Insights from Research on CDC23 Expression and Function |
| title_fullStr | Molecular Regulation of Porcine Skeletal Muscle Development: Insights from Research on CDC23 Expression and Function |
| title_full_unstemmed | Molecular Regulation of Porcine Skeletal Muscle Development: Insights from Research on CDC23 Expression and Function |
| title_short | Molecular Regulation of Porcine Skeletal Muscle Development: Insights from Research on CDC23 Expression and Function |
| title_sort | molecular regulation of porcine skeletal muscle development insights from research on cdc23 expression and function |
| topic | CDC23 porcine satellite cells (PSCs) myoblast differentiation GSEA myoblast proliferation cell cycle pathway |
| url | https://www.mdpi.com/1422-0067/25/7/3664 |
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