COMPARAÇÃO DE MÉTODOS PARA EXTRAÇÃO DE DNA NA REAÇÃO EM CADEIA DA POLIMERASE PARA DETECÇÃO DE Salmonella enterica SOROVAR Enteritidis EM PRODUTOS AVÍCOLAS
The identification of Salmonella spp. in food samples by microbiological diagnosis is time consuming, with approximately five different stages, requiring about 120 hours until the final result. The utilization of the polymerase chain reaction technique (PCR) can reduce this time, but substances pres...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Universidade Federal de Goiás
2011-03-01
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| Series: | Ciência Animal Brasileira |
| Subjects: | |
| Online Access: | http://www.revistas.ufg.br/index.php/vet/article/view/3774 |
| _version_ | 1819118026925015040 |
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| author | Raphael Lucio Andreatti Filho Guilherme Augusto Marietto Gonçalves Adriano Sakai Okamoto Edna Teresa de Lima |
| author_facet | Raphael Lucio Andreatti Filho Guilherme Augusto Marietto Gonçalves Adriano Sakai Okamoto Edna Teresa de Lima |
| author_sort | Raphael Lucio Andreatti Filho |
| collection | DOAJ |
| description | The identification of Salmonella spp. in food samples by microbiological diagnosis is time consuming, with approximately five different stages, requiring about 120 hours until the final result. The utilization of the polymerase chain reaction technique (PCR) can reduce this time, but substances present in samples may affect the reaction. The present work aimed to compare DNA extraction by thermic treatment and by the use of cetyltrimethil ammonium bromide (CTAB), in products originated from poultry houses corresponding to raw material (meat meal) and experimentally contaminated drag swabs. Materials obtained from the extractions were submitted to PCR, utilizing a pair of initiator oligonucleotides for amplification of Sdf 1 gene fragments. Comparing the methods of extraction, it was observed that when CTAB was employed, SE was detected in 70% of meat meal and in 80% of drag swabs, while the thermic treatment method yielded positive results in 20% of meat meal and in 40% of drag swabs. SE was detected under both methods utilized for DNA extraction, but the use of CTAB detected a greater number of positive samples, compared with thermal treatment. |
| first_indexed | 2024-12-22T05:42:20Z |
| format | Article |
| id | doaj.art-e08483305995490983a833b9aaaec7fb |
| institution | Directory Open Access Journal |
| issn | 1518-2797 1089-6891 |
| language | English |
| last_indexed | 2024-12-22T05:42:20Z |
| publishDate | 2011-03-01 |
| publisher | Universidade Federal de Goiás |
| record_format | Article |
| series | Ciência Animal Brasileira |
| spelling | doaj.art-e08483305995490983a833b9aaaec7fb2022-12-21T18:37:09ZengUniversidade Federal de GoiásCiência Animal Brasileira1518-27971089-68912011-03-01121115119COMPARAÇÃO DE MÉTODOS PARA EXTRAÇÃO DE DNA NA REAÇÃO EM CADEIA DA POLIMERASE PARA DETECÇÃO DE Salmonella enterica SOROVAR Enteritidis EM PRODUTOS AVÍCOLASRaphael Lucio Andreatti FilhoGuilherme Augusto Marietto GonçalvesAdriano Sakai OkamotoEdna Teresa de LimaThe identification of Salmonella spp. in food samples by microbiological diagnosis is time consuming, with approximately five different stages, requiring about 120 hours until the final result. The utilization of the polymerase chain reaction technique (PCR) can reduce this time, but substances present in samples may affect the reaction. The present work aimed to compare DNA extraction by thermic treatment and by the use of cetyltrimethil ammonium bromide (CTAB), in products originated from poultry houses corresponding to raw material (meat meal) and experimentally contaminated drag swabs. Materials obtained from the extractions were submitted to PCR, utilizing a pair of initiator oligonucleotides for amplification of Sdf 1 gene fragments. Comparing the methods of extraction, it was observed that when CTAB was employed, SE was detected in 70% of meat meal and in 80% of drag swabs, while the thermic treatment method yielded positive results in 20% of meat meal and in 40% of drag swabs. SE was detected under both methods utilized for DNA extraction, but the use of CTAB detected a greater number of positive samples, compared with thermal treatment.http://www.revistas.ufg.br/index.php/vet/article/view/3774PCRSalmonella EnteritidischickenDNA extractionCTAB. |
| spellingShingle | Raphael Lucio Andreatti Filho Guilherme Augusto Marietto Gonçalves Adriano Sakai Okamoto Edna Teresa de Lima COMPARAÇÃO DE MÉTODOS PARA EXTRAÇÃO DE DNA NA REAÇÃO EM CADEIA DA POLIMERASE PARA DETECÇÃO DE Salmonella enterica SOROVAR Enteritidis EM PRODUTOS AVÍCOLAS Ciência Animal Brasileira PCR Salmonella Enteritidis chicken DNA extraction CTAB. |
| title | COMPARAÇÃO DE MÉTODOS PARA EXTRAÇÃO DE DNA NA REAÇÃO EM CADEIA DA POLIMERASE PARA DETECÇÃO DE Salmonella enterica SOROVAR Enteritidis EM PRODUTOS AVÍCOLAS |
| title_full | COMPARAÇÃO DE MÉTODOS PARA EXTRAÇÃO DE DNA NA REAÇÃO EM CADEIA DA POLIMERASE PARA DETECÇÃO DE Salmonella enterica SOROVAR Enteritidis EM PRODUTOS AVÍCOLAS |
| title_fullStr | COMPARAÇÃO DE MÉTODOS PARA EXTRAÇÃO DE DNA NA REAÇÃO EM CADEIA DA POLIMERASE PARA DETECÇÃO DE Salmonella enterica SOROVAR Enteritidis EM PRODUTOS AVÍCOLAS |
| title_full_unstemmed | COMPARAÇÃO DE MÉTODOS PARA EXTRAÇÃO DE DNA NA REAÇÃO EM CADEIA DA POLIMERASE PARA DETECÇÃO DE Salmonella enterica SOROVAR Enteritidis EM PRODUTOS AVÍCOLAS |
| title_short | COMPARAÇÃO DE MÉTODOS PARA EXTRAÇÃO DE DNA NA REAÇÃO EM CADEIA DA POLIMERASE PARA DETECÇÃO DE Salmonella enterica SOROVAR Enteritidis EM PRODUTOS AVÍCOLAS |
| title_sort | comparacao de metodos para extracao de dna na reacao em cadeia da polimerase para deteccao de salmonella enterica sorovar enteritidis em produtos avicolas |
| topic | PCR Salmonella Enteritidis chicken DNA extraction CTAB. |
| url | http://www.revistas.ufg.br/index.php/vet/article/view/3774 |
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