Proteomic insights into changes in grapevine wood in response to esca proper and apoplexy
Among fungal grapevine trunk diseases, esca proper poses a significant threat for viticulture. Apoplexy, mainly occurring on grapevines affected by esca proper, is also a threat. To verify if different responses are activated in the woody tissues of apoplectic (A) and esca proper-affected (E) vines,...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Firenze University Press
2014-04-01
|
| Series: | Phytopathologia Mediterranea |
| Subjects: | |
| Online Access: | https://oajournals.fupress.net/index.php/pm/article/view/5561 |
| _version_ | 1818921446443843584 |
|---|---|
| author | Maryline MAGNIN-ROBERT Alessandro SPAGNOLO Tchilabalo DILEZITOKO ALAYI Clara CILINDRE Laurence MERCIER Christine SCHAEFFER-REISS Alain VAN DORSSELAER Christophe CLÉMENT Florence FONTAINE |
| author_facet | Maryline MAGNIN-ROBERT Alessandro SPAGNOLO Tchilabalo DILEZITOKO ALAYI Clara CILINDRE Laurence MERCIER Christine SCHAEFFER-REISS Alain VAN DORSSELAER Christophe CLÉMENT Florence FONTAINE |
| author_sort | Maryline MAGNIN-ROBERT |
| collection | DOAJ |
| description | Among fungal grapevine trunk diseases, esca proper poses a significant threat for viticulture. Apoplexy, mainly occurring on grapevines affected by esca proper, is also a threat. To verify if different responses are activated in the woody tissues of apoplectic (A) and esca proper-affected (E) vines, two-dimensional gel electrophoresis coupled to mass spectrometry analysis was used to examine changes in the trunk wood of E and A field-grown plants. Asymptomatic and black streaked trunk (BST) wood from A and E plants were compared to asymptomatic and BST wood of control plants. Twenty-seven differentially expressed protein spots were identified. For eleven targeted proteins, expression of the relative transcripts was also monitored by qRT-PCR. Hierarchical tree clustering revealed differences in the distribution of spots containing carbohydrate metabolism proteins and heat shock proteins between asymptomatic- and BST-wood of grapevine, irrespective of the type of plant examined (control or diseased grapevines). Asymptomatic wood was mainly characterized by down-expression of proteins involved in cell growth and defense responses. The proteome of BST wood, characterized by extensive presence of grapevine trunk disease agents, revealed over-expression of proteins involved in defense. There was no evidence of strong different response in the trunk wood of apoplectic and esca proper affected plants. This could mean that, despite the different feature of these external crown symptoms, grapevine responses at trunk wood level is very similar in both cases. This plant response might therefore either simply be due to the fact that plants can react in the same way to different stresses, or that apoplexy represents a different effect provoked by the same cause or causal agent(s). |
| first_indexed | 2024-12-20T01:37:47Z |
| format | Article |
| id | doaj.art-e08867dd0ca947e2a35165b595d519e1 |
| institution | Directory Open Access Journal |
| issn | 0031-9465 1593-2095 |
| language | English |
| last_indexed | 2024-12-20T01:37:47Z |
| publishDate | 2014-04-01 |
| publisher | Firenze University Press |
| record_format | Article |
| series | Phytopathologia Mediterranea |
| spelling | doaj.art-e08867dd0ca947e2a35165b595d519e12022-12-21T19:57:58ZengFirenze University PressPhytopathologia Mediterranea0031-94651593-20952014-04-0153110.14601/Phytopathol_Mediterr-1291312235Proteomic insights into changes in grapevine wood in response to esca proper and apoplexyMaryline MAGNIN-ROBERT0Alessandro SPAGNOLO1Tchilabalo DILEZITOKO ALAYI2Clara CILINDRE3Laurence MERCIER4Christine SCHAEFFER-REISS5Alain VAN DORSSELAER6Christophe CLÉMENT7Florence FONTAINE8Université de Reims Champagne Ardenne, Laboratoire Stress, Défenses et Reproduction des Plantes, ReimsUniversité de Reims Champagne-Ardenne, Laboratoire Stress, Défenses et Reproduction des Plantes, ReimsUniversité de Strasbourg, IPHC, UMR 7178, Laboratoire de Spectrométrie de Masse BioorganiqueUniversité de Reims Champagne Ardenne, Laboratoire d’Œnologie et Chimie AppliquéeMoët & Chandon companyUniversité de Strasbourg, IPHC, UMR 7178, Laboratoire de Spectrométrie de Masse BioorganiqueUniversité de Strasbourg, IPHC, UMR 7178, Laboratoire de Spectrométrie de Masse BioorganiqueUniversité de Reims Champagne-Ardenne, Laboratoire Stress, Défenses et Reproduction des Plantes, ReimsUniversité de Reims Champagne-Ardenne, Laboratoire Stress, Défenses et Reproduction des Plantes, ReimsAmong fungal grapevine trunk diseases, esca proper poses a significant threat for viticulture. Apoplexy, mainly occurring on grapevines affected by esca proper, is also a threat. To verify if different responses are activated in the woody tissues of apoplectic (A) and esca proper-affected (E) vines, two-dimensional gel electrophoresis coupled to mass spectrometry analysis was used to examine changes in the trunk wood of E and A field-grown plants. Asymptomatic and black streaked trunk (BST) wood from A and E plants were compared to asymptomatic and BST wood of control plants. Twenty-seven differentially expressed protein spots were identified. For eleven targeted proteins, expression of the relative transcripts was also monitored by qRT-PCR. Hierarchical tree clustering revealed differences in the distribution of spots containing carbohydrate metabolism proteins and heat shock proteins between asymptomatic- and BST-wood of grapevine, irrespective of the type of plant examined (control or diseased grapevines). Asymptomatic wood was mainly characterized by down-expression of proteins involved in cell growth and defense responses. The proteome of BST wood, characterized by extensive presence of grapevine trunk disease agents, revealed over-expression of proteins involved in defense. There was no evidence of strong different response in the trunk wood of apoplectic and esca proper affected plants. This could mean that, despite the different feature of these external crown symptoms, grapevine responses at trunk wood level is very similar in both cases. This plant response might therefore either simply be due to the fact that plants can react in the same way to different stresses, or that apoplexy represents a different effect provoked by the same cause or causal agent(s).https://oajournals.fupress.net/index.php/pm/article/view/5561grapevineproteomicsqRT-PCRtrunk diseases |
| spellingShingle | Maryline MAGNIN-ROBERT Alessandro SPAGNOLO Tchilabalo DILEZITOKO ALAYI Clara CILINDRE Laurence MERCIER Christine SCHAEFFER-REISS Alain VAN DORSSELAER Christophe CLÉMENT Florence FONTAINE Proteomic insights into changes in grapevine wood in response to esca proper and apoplexy Phytopathologia Mediterranea grapevine proteomics qRT-PCR trunk diseases |
| title | Proteomic insights into changes in grapevine wood in response to esca proper and apoplexy |
| title_full | Proteomic insights into changes in grapevine wood in response to esca proper and apoplexy |
| title_fullStr | Proteomic insights into changes in grapevine wood in response to esca proper and apoplexy |
| title_full_unstemmed | Proteomic insights into changes in grapevine wood in response to esca proper and apoplexy |
| title_short | Proteomic insights into changes in grapevine wood in response to esca proper and apoplexy |
| title_sort | proteomic insights into changes in grapevine wood in response to esca proper and apoplexy |
| topic | grapevine proteomics qRT-PCR trunk diseases |
| url | https://oajournals.fupress.net/index.php/pm/article/view/5561 |
| work_keys_str_mv | AT marylinemagninrobert proteomicinsightsintochangesingrapevinewoodinresponsetoescaproperandapoplexy AT alessandrospagnolo proteomicinsightsintochangesingrapevinewoodinresponsetoescaproperandapoplexy AT tchilabalodilezitokoalayi proteomicinsightsintochangesingrapevinewoodinresponsetoescaproperandapoplexy AT claracilindre proteomicinsightsintochangesingrapevinewoodinresponsetoescaproperandapoplexy AT laurencemercier proteomicinsightsintochangesingrapevinewoodinresponsetoescaproperandapoplexy AT christineschaefferreiss proteomicinsightsintochangesingrapevinewoodinresponsetoescaproperandapoplexy AT alainvandorsselaer proteomicinsightsintochangesingrapevinewoodinresponsetoescaproperandapoplexy AT christopheclement proteomicinsightsintochangesingrapevinewoodinresponsetoescaproperandapoplexy AT florencefontaine proteomicinsightsintochangesingrapevinewoodinresponsetoescaproperandapoplexy |