Separation and Quantification of Selected Sapogenins Extracted from Nettle, White Dead-Nettle, Common Soapwort and Washnut
Saponins are an important group of secondary metabolites naturally occurring in plants with important properties like: antibacterial, antiviral and antifungal. Moreover, they are widely used in the cosmetic industry and household chemistry. The sapogenins are saponin hydrolyses products, frequently...
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MDPI AG
2021-12-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/24/7705 |
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| author | Magdalena Ligor Anna Kiełbasa Ileana-Andreea Ratiu Bogusław Buszewski |
| author_facet | Magdalena Ligor Anna Kiełbasa Ileana-Andreea Ratiu Bogusław Buszewski |
| author_sort | Magdalena Ligor |
| collection | DOAJ |
| description | Saponins are an important group of secondary metabolites naturally occurring in plants with important properties like: antibacterial, antiviral and antifungal. Moreover, they are widely used in the cosmetic industry and household chemistry. The sapogenins are saponin hydrolyses products, frequently used to facilitate saponin detection. In the present study, an improved methodology for isolation and separation of five sapogenins extracted from nettle (<i>Urtica dioica</i> L.), white dead-nettle (<i>Lamium album</i> L.), common soapwort (<i>Saponaria officinalis</i> L.) and washnut (<i>Sapindus mukorossi</i> Gaertn.) was developed using ultra-high-performance liquid chromatography with an evaporative light-scattering detector (UHPLC-ELSD). Based on quantitative analysis, the highest content of hederagenin (999.1 ± 6.3 µg/g) and oleanolic acid (386.5 ± 27.7 µg/g) was found in washnut extracts. Good recoveries (71% ± 6 up to 99% ± 8) were achieved for four investigated targets, while just 22.2% ± 0.5 was obtained for the fifth one. Moreover, hederagenin and oleanolic acid of whose highest amount was detected in washnut (999.1 ± 6.3 µg/g and 386.5 ± 27.7 µg/g, respectively) were subject to another approach. Consequently, liquid chromatography coupled mass spectrometry (LC/MS) with multiple reaction monitoring mode (MRM) was used as an additional technique for fast and simultaneous identification of the mentioned targets. |
| first_indexed | 2024-03-10T03:28:46Z |
| format | Article |
| id | doaj.art-e088d4131ca0450591fc6f1beb338b5a |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T03:28:46Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-e088d4131ca0450591fc6f1beb338b5a2023-11-23T09:48:03ZengMDPI AGMolecules1420-30492021-12-012624770510.3390/molecules26247705Separation and Quantification of Selected Sapogenins Extracted from Nettle, White Dead-Nettle, Common Soapwort and WashnutMagdalena Ligor0Anna Kiełbasa1Ileana-Andreea Ratiu2Bogusław Buszewski3Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, 7 Gagarina Str., 87-100 Torun, PolandDepartment of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, 7 Gagarina Str., 87-100 Torun, Poland“Raluca Ripan” Institute for Research in Chemistry, Babes-Bolyai University, 30 Fantanele Str., RO-400239 Cluj-Napoca, RomaniaDepartment of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, 7 Gagarina Str., 87-100 Torun, PolandSaponins are an important group of secondary metabolites naturally occurring in plants with important properties like: antibacterial, antiviral and antifungal. Moreover, they are widely used in the cosmetic industry and household chemistry. The sapogenins are saponin hydrolyses products, frequently used to facilitate saponin detection. In the present study, an improved methodology for isolation and separation of five sapogenins extracted from nettle (<i>Urtica dioica</i> L.), white dead-nettle (<i>Lamium album</i> L.), common soapwort (<i>Saponaria officinalis</i> L.) and washnut (<i>Sapindus mukorossi</i> Gaertn.) was developed using ultra-high-performance liquid chromatography with an evaporative light-scattering detector (UHPLC-ELSD). Based on quantitative analysis, the highest content of hederagenin (999.1 ± 6.3 µg/g) and oleanolic acid (386.5 ± 27.7 µg/g) was found in washnut extracts. Good recoveries (71% ± 6 up to 99% ± 8) were achieved for four investigated targets, while just 22.2% ± 0.5 was obtained for the fifth one. Moreover, hederagenin and oleanolic acid of whose highest amount was detected in washnut (999.1 ± 6.3 µg/g and 386.5 ± 27.7 µg/g, respectively) were subject to another approach. Consequently, liquid chromatography coupled mass spectrometry (LC/MS) with multiple reaction monitoring mode (MRM) was used as an additional technique for fast and simultaneous identification of the mentioned targets.https://www.mdpi.com/1420-3049/26/24/7705plant materialsapogeninsextraction liquid chromatographyantioxidant activity |
| spellingShingle | Magdalena Ligor Anna Kiełbasa Ileana-Andreea Ratiu Bogusław Buszewski Separation and Quantification of Selected Sapogenins Extracted from Nettle, White Dead-Nettle, Common Soapwort and Washnut Molecules plant material sapogenins extraction liquid chromatography antioxidant activity |
| title | Separation and Quantification of Selected Sapogenins Extracted from Nettle, White Dead-Nettle, Common Soapwort and Washnut |
| title_full | Separation and Quantification of Selected Sapogenins Extracted from Nettle, White Dead-Nettle, Common Soapwort and Washnut |
| title_fullStr | Separation and Quantification of Selected Sapogenins Extracted from Nettle, White Dead-Nettle, Common Soapwort and Washnut |
| title_full_unstemmed | Separation and Quantification of Selected Sapogenins Extracted from Nettle, White Dead-Nettle, Common Soapwort and Washnut |
| title_short | Separation and Quantification of Selected Sapogenins Extracted from Nettle, White Dead-Nettle, Common Soapwort and Washnut |
| title_sort | separation and quantification of selected sapogenins extracted from nettle white dead nettle common soapwort and washnut |
| topic | plant material sapogenins extraction liquid chromatography antioxidant activity |
| url | https://www.mdpi.com/1420-3049/26/24/7705 |
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