Querying Recombination Junctions of Replication-Competent Adeno-Associated Viruses in Gene Therapy Vector Preparations with Single Molecule, Real-Time Sequencing

Querying Recombination Junctions of Replication-Competent Adeno-Associated Viruses in Gene Therapy Vector Preparations with Single Molecule, Real-Time Sequencing

Clinical-grade preparations of adeno-associated virus (AAV) vectors used for gene therapy typically undergo a series of diagnostics to determine titer, purity, homogeneity, and the presence of DNA contaminants. One type of contaminant that remains poorly investigated is replication-competent (rc)AAV...

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Bibliographic Details
Main Authors: Mitchell Yip, Jing Chen, Yan Zhi, Ngoc Tam Tran, Suk Namkung, Eric Pastor, Guangping Gao, Phillip W. L. Tai
Format: Article
Language:English
Published: MDPI AG 2023-05-01
Series:Viruses
Subjects:
adeno-associated virus
replication-competent AAV
single molecule
real-time sequencing
AAV-genome population sequencing
Online Access:https://www.mdpi.com/1999-4915/15/6/1228

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