Isolation and Identification of Bioactive Compounds from <i>Bidens</i> spp. Using HPLC-DAD and GC-MS Analysis and Their Biological Activity as Anticancer Molecules
The genus <i>Bidens</i> a member of family Compositae, is widely documented as an ethno-medicinally important genus of plants. In the present study, anticancer potential of three ethno-medicinally important species i.e., <i>B. bipinnata</i>, <i>B. biternata</i> an...
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MDPI AG
2022-03-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/6/1927 |
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| author | Kulsoom Zahara Yamin Bibi Saadia Masood Sobia Nisa Ahmad Sher Naushad Ali Sunjeet Kumar Abdul Qayyum Waseem Ahmed Rokayya Sami Amina A. M. Al-Mushhin Amani H. Aljahani |
| author_facet | Kulsoom Zahara Yamin Bibi Saadia Masood Sobia Nisa Ahmad Sher Naushad Ali Sunjeet Kumar Abdul Qayyum Waseem Ahmed Rokayya Sami Amina A. M. Al-Mushhin Amani H. Aljahani |
| author_sort | Kulsoom Zahara |
| collection | DOAJ |
| description | The genus <i>Bidens</i> a member of family Compositae, is widely documented as an ethno-medicinally important genus of plants. In the present study, anticancer potential of three ethno-medicinally important species i.e., <i>B. bipinnata</i>, <i>B. biternata</i> and <i>B. pilosa</i> were tested. For in-vitro evaluation, an MTT (Thiazolyl blue tetrazolium bromide) assay was performed against cervical cancer cells (HeLa), hepatocellular carcinoma (HepG), and adenocarcinoma human alveolar basal epithelial cells (A549). For in vivo evaluation, <i>Artemia salina</i>, <i>Danio rerio,</i> and <i>Caenorhabditis elegans</i> were used. Among all the tested extracts, the ethanol extract of <i>B. biternata</i> appeared to have highest anticancer activity, and the compounds responsible for this activity were identified to be Tris (2,4-di-<i>tert</i>-butylphenyl), 4-hydroxy-2,4′-dimethoxychalcone, and 2,4-di-<i>tert</i>-butylphenol. This is the first report of the isolation of Tris (2,4-di-<i>tert</i>-butylphenyl) phosphate from the genus <i>Bidens</i> and the first report of 4-hydroxy-2,4′-dimethoxychalcone and 2,4-di-<i>tert</i>-butylphenol from <i>B. biternata</i>. Among the isolated compounds, 4-hydroxy-2,4′-dimethoxychalcone showed the highest anticancer activity with an LD50 value of 236.7 µg/mL. Therefore, this compound carries promising potential for being established as a pharmaceutical for chemoprevention and chemotherapy. |
| first_indexed | 2024-03-09T13:10:05Z |
| format | Article |
| id | doaj.art-e089eb3fe9994c65ba5fc7d0a12a3767 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T13:10:05Z |
| publishDate | 2022-03-01 |
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| record_format | Article |
| series | Molecules |
| spelling | doaj.art-e089eb3fe9994c65ba5fc7d0a12a37672023-11-30T21:43:22ZengMDPI AGMolecules1420-30492022-03-01276192710.3390/molecules27061927Isolation and Identification of Bioactive Compounds from <i>Bidens</i> spp. Using HPLC-DAD and GC-MS Analysis and Their Biological Activity as Anticancer MoleculesKulsoom Zahara0Yamin Bibi1Saadia Masood2Sobia Nisa3Ahmad Sher4Naushad Ali5Sunjeet Kumar6Abdul Qayyum7Waseem Ahmed8Rokayya Sami9Amina A. M. Al-Mushhin10Amani H. Aljahani11Department of Botany, PMAS-Arid Agriculture University Rawalpindi, Rawalpindi 46300, PakistanDepartment of Botany, PMAS-Arid Agriculture University Rawalpindi, Rawalpindi 46300, PakistanDepartment of Statistics & Mathematics, PMAS-Arid Agriculture University Rawalpindi, Rawalpindi 46300, PakistanDepartment of Microbiology, The University of Haripur, Haripur 22620, PakistanCollege of Agriculture, Bahauddin Zakariya University, Bahadur Sub Campus, Layyah 31200, PakistanDepartment of Plant Breeding & Genetics, The University of Haripur, Haripur 22620, PakistanKey Laboratory for Quality Regulation of Tropical Horticultural Crops of Hainan Province, School of Horticulture, Hainan University, Haikou 570228, ChinaDepartment of Agronomy, The University of Haripur, Haripur 22620, PakistanDepartment of Horticulture, The University of Haripur, Haripur 22620, PakistanDepartment of Food Science and Nutrition, College of Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaDepartment of Biology, College of Science and Humanities in Al-Kharj, Prince Sattam Bin Abdulaziz University, Al-Kharj 11942, Saudi ArabiaDepartment of Physical Sport Science, College of Education, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi ArabiaThe genus <i>Bidens</i> a member of family Compositae, is widely documented as an ethno-medicinally important genus of plants. In the present study, anticancer potential of three ethno-medicinally important species i.e., <i>B. bipinnata</i>, <i>B. biternata</i> and <i>B. pilosa</i> were tested. For in-vitro evaluation, an MTT (Thiazolyl blue tetrazolium bromide) assay was performed against cervical cancer cells (HeLa), hepatocellular carcinoma (HepG), and adenocarcinoma human alveolar basal epithelial cells (A549). For in vivo evaluation, <i>Artemia salina</i>, <i>Danio rerio,</i> and <i>Caenorhabditis elegans</i> were used. Among all the tested extracts, the ethanol extract of <i>B. biternata</i> appeared to have highest anticancer activity, and the compounds responsible for this activity were identified to be Tris (2,4-di-<i>tert</i>-butylphenyl), 4-hydroxy-2,4′-dimethoxychalcone, and 2,4-di-<i>tert</i>-butylphenol. This is the first report of the isolation of Tris (2,4-di-<i>tert</i>-butylphenyl) phosphate from the genus <i>Bidens</i> and the first report of 4-hydroxy-2,4′-dimethoxychalcone and 2,4-di-<i>tert</i>-butylphenol from <i>B. biternata</i>. Among the isolated compounds, 4-hydroxy-2,4′-dimethoxychalcone showed the highest anticancer activity with an LD50 value of 236.7 µg/mL. Therefore, this compound carries promising potential for being established as a pharmaceutical for chemoprevention and chemotherapy.https://www.mdpi.com/1420-3049/27/6/1927anticancer<i>Bidens</i>zebrafishbrine shrimp |
| spellingShingle | Kulsoom Zahara Yamin Bibi Saadia Masood Sobia Nisa Ahmad Sher Naushad Ali Sunjeet Kumar Abdul Qayyum Waseem Ahmed Rokayya Sami Amina A. M. Al-Mushhin Amani H. Aljahani Isolation and Identification of Bioactive Compounds from <i>Bidens</i> spp. Using HPLC-DAD and GC-MS Analysis and Their Biological Activity as Anticancer Molecules Molecules anticancer <i>Bidens</i> zebrafish brine shrimp |
| title | Isolation and Identification of Bioactive Compounds from <i>Bidens</i> spp. Using HPLC-DAD and GC-MS Analysis and Their Biological Activity as Anticancer Molecules |
| title_full | Isolation and Identification of Bioactive Compounds from <i>Bidens</i> spp. Using HPLC-DAD and GC-MS Analysis and Their Biological Activity as Anticancer Molecules |
| title_fullStr | Isolation and Identification of Bioactive Compounds from <i>Bidens</i> spp. Using HPLC-DAD and GC-MS Analysis and Their Biological Activity as Anticancer Molecules |
| title_full_unstemmed | Isolation and Identification of Bioactive Compounds from <i>Bidens</i> spp. Using HPLC-DAD and GC-MS Analysis and Their Biological Activity as Anticancer Molecules |
| title_short | Isolation and Identification of Bioactive Compounds from <i>Bidens</i> spp. Using HPLC-DAD and GC-MS Analysis and Their Biological Activity as Anticancer Molecules |
| title_sort | isolation and identification of bioactive compounds from i bidens i spp using hplc dad and gc ms analysis and their biological activity as anticancer molecules |
| topic | anticancer <i>Bidens</i> zebrafish brine shrimp |
| url | https://www.mdpi.com/1420-3049/27/6/1927 |
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