Efficiency of SSR markers for determining the origin of melon plantlets derived through unfertilized ovary culture
The effects of temperature pre-treatment, thidiazuron, naphthaleneacetic acid, and 6-benzylaminopurine on in vitro gynogenic plant production from un-pollinated melon (Cucumis melo L.) ovaries were investigated. Simple sequence repeat (SSR) marker analysis was conducted to identify the homozygous di...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2011-03-01
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| Series: | Horticultural Science |
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| Online Access: | https://hortsci.agriculturejournals.cz/artkey/hor-201101-0005_efficiency-of-ssr-markers-for-determining-the-origin-of-melon-plantlets-derived-through-unfertilized-ovary-cult.php |
| _version_ | 1797899088526573568 |
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| author | A.A. Malik Li Cui Shuxia Zhang Jin-Feng Chen |
| author_facet | A.A. Malik Li Cui Shuxia Zhang Jin-Feng Chen |
| author_sort | A.A. Malik |
| collection | DOAJ |
| description | The effects of temperature pre-treatment, thidiazuron, naphthaleneacetic acid, and 6-benzylaminopurine on in vitro gynogenic plant production from un-pollinated melon (Cucumis melo L.) ovaries were investigated. Simple sequence repeat (SSR) marker analysis was conducted to identify the homozygous diploid individuals. The temperature pre-treatment (4°C) for 4 days increased embryo formation frequency (63.3%) significantly. Addition of thidiazuron (0.04 and 0.02 mg/l) in the induction medium significantly increased the number of responding ovaries (46.6%, 65.83%), respectively. The maximum number of plantlet regeneration (22.5%) was achieved by culturing the ovary derived embryos on Murashigue and Skoog medium (MS medium) supplement with 0.6 mg/l 6-benzylaminopurine. Spontaneous doubled haploids originated directly through embryogenesis were subjected to genetic analysis using SSR molecular marker with 23 primers pair for homozygosity. SSR markers with microsatellite CMGA172, confirmed that the alleles in the parental material were also present in the gynogenic plantlets, but amplified only two alleles as compared to four alleles of the heterozygous parent material at same locus. Therefore these regenerated plantlets were consider homozygous and produced through a process of gametophytic embryogenesis. |
| first_indexed | 2024-04-10T08:23:15Z |
| format | Article |
| id | doaj.art-e0920412e63f401bb92d3aa77765ae5a |
| institution | Directory Open Access Journal |
| issn | 0862-867X 1805-9333 |
| language | English |
| last_indexed | 2024-04-10T08:23:15Z |
| publishDate | 2011-03-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Horticultural Science |
| spelling | doaj.art-e0920412e63f401bb92d3aa77765ae5a2023-02-23T03:34:40ZengCzech Academy of Agricultural SciencesHorticultural Science0862-867X1805-93332011-03-01381273410.17221/47/2010-HORTSCIhor-201101-0005Efficiency of SSR markers for determining the origin of melon plantlets derived through unfertilized ovary cultureA.A. Malik0Li Cui1Shuxia Zhang2Jin-Feng Chen3State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, ChinaState Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, ChinaState Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, ChinaState Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, ChinaThe effects of temperature pre-treatment, thidiazuron, naphthaleneacetic acid, and 6-benzylaminopurine on in vitro gynogenic plant production from un-pollinated melon (Cucumis melo L.) ovaries were investigated. Simple sequence repeat (SSR) marker analysis was conducted to identify the homozygous diploid individuals. The temperature pre-treatment (4°C) for 4 days increased embryo formation frequency (63.3%) significantly. Addition of thidiazuron (0.04 and 0.02 mg/l) in the induction medium significantly increased the number of responding ovaries (46.6%, 65.83%), respectively. The maximum number of plantlet regeneration (22.5%) was achieved by culturing the ovary derived embryos on Murashigue and Skoog medium (MS medium) supplement with 0.6 mg/l 6-benzylaminopurine. Spontaneous doubled haploids originated directly through embryogenesis were subjected to genetic analysis using SSR molecular marker with 23 primers pair for homozygosity. SSR markers with microsatellite CMGA172, confirmed that the alleles in the parental material were also present in the gynogenic plantlets, but amplified only two alleles as compared to four alleles of the heterozygous parent material at same locus. Therefore these regenerated plantlets were consider homozygous and produced through a process of gametophytic embryogenesis.https://hortsci.agriculturejournals.cz/artkey/hor-201101-0005_efficiency-of-ssr-markers-for-determining-the-origin-of-melon-plantlets-derived-through-unfertilized-ovary-cult.phpcucumis melo l.in vitro gynogenesisembryo formationmicrosatellites markers |
| spellingShingle | A.A. Malik Li Cui Shuxia Zhang Jin-Feng Chen Efficiency of SSR markers for determining the origin of melon plantlets derived through unfertilized ovary culture Horticultural Science cucumis melo l. in vitro gynogenesis embryo formation microsatellites markers |
| title | Efficiency of SSR markers for determining the origin of melon plantlets derived through unfertilized ovary culture |
| title_full | Efficiency of SSR markers for determining the origin of melon plantlets derived through unfertilized ovary culture |
| title_fullStr | Efficiency of SSR markers for determining the origin of melon plantlets derived through unfertilized ovary culture |
| title_full_unstemmed | Efficiency of SSR markers for determining the origin of melon plantlets derived through unfertilized ovary culture |
| title_short | Efficiency of SSR markers for determining the origin of melon plantlets derived through unfertilized ovary culture |
| title_sort | efficiency of ssr markers for determining the origin of melon plantlets derived through unfertilized ovary culture |
| topic | cucumis melo l. in vitro gynogenesis embryo formation microsatellites markers |
| url | https://hortsci.agriculturejournals.cz/artkey/hor-201101-0005_efficiency-of-ssr-markers-for-determining-the-origin-of-melon-plantlets-derived-through-unfertilized-ovary-cult.php |
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