Tissue expression distribution characterization of the host defense peptide dCATH in Anas platyrhynchos
Cathelicidin host defense peptides (cHDPs) widely exist in animals with the functions of innate immunity and acquired immunity. To investigate the tissue and sequential expression patterns of the dCATH gene, which encodes the only cHDPs found in waterfowls, we analyzed the internal organs of healthy...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-01-01
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| Series: | Biotechnology & Biotechnological Equipment |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/13102818.2020.1814163 |
| _version_ | 1818844609365671936 |
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| author | Sanjun Jin Qian Pang Tingting Zhang Min Wang Hao Yang Fangju Liu Yingjie Wang Anshan Shan Xingjun Feng |
| author_facet | Sanjun Jin Qian Pang Tingting Zhang Min Wang Hao Yang Fangju Liu Yingjie Wang Anshan Shan Xingjun Feng |
| author_sort | Sanjun Jin |
| collection | DOAJ |
| description | Cathelicidin host defense peptides (cHDPs) widely exist in animals with the functions of innate immunity and acquired immunity. To investigate the tissue and sequential expression patterns of the dCATH gene, which encodes the only cHDPs found in waterfowls, we analyzed the internal organs of healthy ducks (Shaxoxing Sheldrake) from 1-day-old to 28-day-old raised in the natural environment. The dCATH mRNA expression level across tissues was determined via quantitative real-time polymerase chain reaction (qRT-PCR). Monoclonal antibody against the mature dCATH peptide used in enzyme-linked immunosorbent assay (ELISA) was prepared by the lymphocyte hybridoma technique for the first time. The concentration of the dCATH peptide in tissues was determined by ELISA. It showed that the dCATH mRNA had a clear tissue-specificity and age-dependent expression pattern, and it was highly expressed in the bone marrow and moderately expressed in the liver, kidney, spleen, pancreas and bursa of healthy duck. The peptide levels of dCATH in liver, kidney, spleen, pancreas and bursa changed with the age of duck, and the peptide concentration in these organs was between 88 and 128 ng/g tissue. For the first time, we characterized the concentration of the cHDP dCATH in vivo. Our study may represent new insights into the crosstalk of innate immunity, tissue development and age. |
| first_indexed | 2024-12-19T05:16:29Z |
| format | Article |
| id | doaj.art-e09266e6eca44a95a90a067b23ff6496 |
| institution | Directory Open Access Journal |
| issn | 1310-2818 1314-3530 |
| language | English |
| last_indexed | 2024-12-19T05:16:29Z |
| publishDate | 2020-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Biotechnology & Biotechnological Equipment |
| spelling | doaj.art-e09266e6eca44a95a90a067b23ff64962022-12-21T20:34:38ZengTaylor & Francis GroupBiotechnology & Biotechnological Equipment1310-28181314-35302020-01-0134195596510.1080/13102818.2020.18141631814163Tissue expression distribution characterization of the host defense peptide dCATH in Anas platyrhynchosSanjun Jin0Qian Pang1Tingting Zhang2Min Wang3Hao Yang4Fangju Liu5Yingjie Wang6Anshan Shan7Xingjun Feng8Laboratory of Molecular Nutrition, Institute of Animal Nutrition, Northeast Agricultural UniversityLaboratory of Molecular Nutrition, Institute of Animal Nutrition, Northeast Agricultural UniversityLaboratory of Molecular Nutrition, Institute of Animal Nutrition, Northeast Agricultural UniversityLaboratory of Molecular Nutrition, Institute of Animal Nutrition, Northeast Agricultural UniversityLaboratory of Molecular Nutrition, Institute of Animal Nutrition, Northeast Agricultural UniversityLaboratory of Molecular Nutrition, Institute of Animal Nutrition, Northeast Agricultural UniversityLaboratory of Molecular Nutrition, Institute of Animal Nutrition, Northeast Agricultural UniversityLaboratory of Molecular Nutrition, Institute of Animal Nutrition, Northeast Agricultural UniversityLaboratory of Molecular Nutrition, Institute of Animal Nutrition, Northeast Agricultural UniversityCathelicidin host defense peptides (cHDPs) widely exist in animals with the functions of innate immunity and acquired immunity. To investigate the tissue and sequential expression patterns of the dCATH gene, which encodes the only cHDPs found in waterfowls, we analyzed the internal organs of healthy ducks (Shaxoxing Sheldrake) from 1-day-old to 28-day-old raised in the natural environment. The dCATH mRNA expression level across tissues was determined via quantitative real-time polymerase chain reaction (qRT-PCR). Monoclonal antibody against the mature dCATH peptide used in enzyme-linked immunosorbent assay (ELISA) was prepared by the lymphocyte hybridoma technique for the first time. The concentration of the dCATH peptide in tissues was determined by ELISA. It showed that the dCATH mRNA had a clear tissue-specificity and age-dependent expression pattern, and it was highly expressed in the bone marrow and moderately expressed in the liver, kidney, spleen, pancreas and bursa of healthy duck. The peptide levels of dCATH in liver, kidney, spleen, pancreas and bursa changed with the age of duck, and the peptide concentration in these organs was between 88 and 128 ng/g tissue. For the first time, we characterized the concentration of the cHDP dCATH in vivo. Our study may represent new insights into the crosstalk of innate immunity, tissue development and age.http://dx.doi.org/10.1080/13102818.2020.1814163host defense peptideduckgene expressionmonoclonal antibodypeptide concentration |
| spellingShingle | Sanjun Jin Qian Pang Tingting Zhang Min Wang Hao Yang Fangju Liu Yingjie Wang Anshan Shan Xingjun Feng Tissue expression distribution characterization of the host defense peptide dCATH in Anas platyrhynchos Biotechnology & Biotechnological Equipment host defense peptide duck gene expression monoclonal antibody peptide concentration |
| title | Tissue expression distribution characterization of the host defense peptide dCATH in Anas platyrhynchos |
| title_full | Tissue expression distribution characterization of the host defense peptide dCATH in Anas platyrhynchos |
| title_fullStr | Tissue expression distribution characterization of the host defense peptide dCATH in Anas platyrhynchos |
| title_full_unstemmed | Tissue expression distribution characterization of the host defense peptide dCATH in Anas platyrhynchos |
| title_short | Tissue expression distribution characterization of the host defense peptide dCATH in Anas platyrhynchos |
| title_sort | tissue expression distribution characterization of the host defense peptide dcath in anas platyrhynchos |
| topic | host defense peptide duck gene expression monoclonal antibody peptide concentration |
| url | http://dx.doi.org/10.1080/13102818.2020.1814163 |
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