Biological activities of Umbilicaria crustulosa (Ach.) frey acetone extract
This paper reports for the first time the effect of an acetone extract of Umbilicaria crustulosa on the micronucleus distribution of human lymphocytes, and on the cholinesterase activity and antioxidant activity by the cupric ion reducing antioxidant capacity (CUPRAC) method. Additionally, the total...
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| Format: | Article |
| Language: | English |
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Serbian Chemical Society
2017-01-01
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| Series: | Journal of the Serbian Chemical Society |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0352-5139/2017/0352-51391700014Z.pdf |
| _version_ | 1828434692187095040 |
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| author | Zlatanović Ivana Stanković Miroslava Stankov-Jovanović Vesna Mitić Violeta Zrnzević Ivana Đorđević Aleksandra Stojanović Gordana |
| author_facet | Zlatanović Ivana Stanković Miroslava Stankov-Jovanović Vesna Mitić Violeta Zrnzević Ivana Đorđević Aleksandra Stojanović Gordana |
| author_sort | Zlatanović Ivana |
| collection | DOAJ |
| description | This paper reports for the first time the effect of an acetone extract of Umbilicaria crustulosa on the micronucleus distribution of human lymphocytes, and on the cholinesterase activity and antioxidant activity by the cupric ion reducing antioxidant capacity (CUPRAC) method. Additionally, the total phenolic compounds (TPC) and the antioxidant properties were estimated via DPPH, ABTS and TRP assays. Moreover, the antibacterial activity against two Gram-positive and three Gram-negative bacteria were determined. Acetone extract of U. crustulosa at concentration of 1 and 2 μg mL-1 decreased a frequency of micronuclei (MN) by 10.8 and 16.8 %, respectively, acting more or slightly less than the synthetic protector amifostine (AMF, WR-2721, 11.4 %, at concentration of 1 μg mL-1). The tested extract did not inhibit cholinesterase activity nor did it exhibit activity toward the examined bacteria. The extract reduced the concentration of DPPH and ABTS radicals by 88.7 and 96.2 %, respectively. Values for total reducing power (TRP) and cupric reducing capacity (CUPRAC) were 0.6197±0.0166 μg ascorbic acid equivalents (AAE) per mg of dry extract, and 19.7641±1.6546 μg trolox equivalents (TE) per mg of dry extract, respectively. The total phenol content was 350.4188 ±14.587 μg gallic acid equivalents (GAE) per mg of dry extract. The results of the present study showed that U. crustulosa acetone extract is a promising candidate for in vivo experiments considering its antioxidant activity and protective effect on human lymphocytes. [Projekat Ministarstva nauke Republike Srbije, br. 172047] |
| first_indexed | 2024-12-10T18:53:54Z |
| format | Article |
| id | doaj.art-e0960764599143b9a275fed79b9dc6cd |
| institution | Directory Open Access Journal |
| issn | 0352-5139 1820-7421 |
| language | English |
| last_indexed | 2024-12-10T18:53:54Z |
| publishDate | 2017-01-01 |
| publisher | Serbian Chemical Society |
| record_format | Article |
| series | Journal of the Serbian Chemical Society |
| spelling | doaj.art-e0960764599143b9a275fed79b9dc6cd2022-12-22T01:37:13ZengSerbian Chemical SocietyJournal of the Serbian Chemical Society0352-51391820-74212017-01-0182214115010.2298/JSC161019014Z0352-51391700014ZBiological activities of Umbilicaria crustulosa (Ach.) frey acetone extractZlatanović Ivana0Stanković Miroslava1Stankov-Jovanović Vesna2Mitić Violeta3Zrnzević Ivana4Đorđević Aleksandra5Stojanović Gordana6Faculty of Science and Mathematics, Department of Chemistry, NišNuclear Facilities of Serbia Vinča, BelgradeFaculty of Science and Mathematics, Department of Chemistry, NišFaculty of Science and Mathematics, Department of Chemistry, NišFaculty of Science and Mathematics, Department of Chemistry, NišFaculty of Science and Mathematics, Department of Chemistry, NišFaculty of Science and Mathematics, Department of Chemistry, NišThis paper reports for the first time the effect of an acetone extract of Umbilicaria crustulosa on the micronucleus distribution of human lymphocytes, and on the cholinesterase activity and antioxidant activity by the cupric ion reducing antioxidant capacity (CUPRAC) method. Additionally, the total phenolic compounds (TPC) and the antioxidant properties were estimated via DPPH, ABTS and TRP assays. Moreover, the antibacterial activity against two Gram-positive and three Gram-negative bacteria were determined. Acetone extract of U. crustulosa at concentration of 1 and 2 μg mL-1 decreased a frequency of micronuclei (MN) by 10.8 and 16.8 %, respectively, acting more or slightly less than the synthetic protector amifostine (AMF, WR-2721, 11.4 %, at concentration of 1 μg mL-1). The tested extract did not inhibit cholinesterase activity nor did it exhibit activity toward the examined bacteria. The extract reduced the concentration of DPPH and ABTS radicals by 88.7 and 96.2 %, respectively. Values for total reducing power (TRP) and cupric reducing capacity (CUPRAC) were 0.6197±0.0166 μg ascorbic acid equivalents (AAE) per mg of dry extract, and 19.7641±1.6546 μg trolox equivalents (TE) per mg of dry extract, respectively. The total phenol content was 350.4188 ±14.587 μg gallic acid equivalents (GAE) per mg of dry extract. The results of the present study showed that U. crustulosa acetone extract is a promising candidate for in vivo experiments considering its antioxidant activity and protective effect on human lymphocytes. [Projekat Ministarstva nauke Republike Srbije, br. 172047]http://www.doiserbia.nb.rs/img/doi/0352-5139/2017/0352-51391700014Z.pdfmicronucleus testantioxidant activitycholinesterase inhibitionantimicrobial activitychemical composition |
| spellingShingle | Zlatanović Ivana Stanković Miroslava Stankov-Jovanović Vesna Mitić Violeta Zrnzević Ivana Đorđević Aleksandra Stojanović Gordana Biological activities of Umbilicaria crustulosa (Ach.) frey acetone extract Journal of the Serbian Chemical Society micronucleus test antioxidant activity cholinesterase inhibition antimicrobial activity chemical composition |
| title | Biological activities of Umbilicaria crustulosa (Ach.) frey acetone extract |
| title_full | Biological activities of Umbilicaria crustulosa (Ach.) frey acetone extract |
| title_fullStr | Biological activities of Umbilicaria crustulosa (Ach.) frey acetone extract |
| title_full_unstemmed | Biological activities of Umbilicaria crustulosa (Ach.) frey acetone extract |
| title_short | Biological activities of Umbilicaria crustulosa (Ach.) frey acetone extract |
| title_sort | biological activities of umbilicaria crustulosa ach frey acetone extract |
| topic | micronucleus test antioxidant activity cholinesterase inhibition antimicrobial activity chemical composition |
| url | http://www.doiserbia.nb.rs/img/doi/0352-5139/2017/0352-51391700014Z.pdf |
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