Molecular Characterization and Antifungal Susceptibility of <i>Aspergillus</i> spp. among Patients with Underlying Lung Diseases
Background. Pulmonary aspergillosis is a lung infection caused by <i>Aspergillus</i> spp., which can cause severe illnesses in immunocompromised patients with underlying lung disease or who have asthma and inhale their spores. This study aimed to screen the antifungal susceptibility of &...
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MDPI AG
2022-09-01
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| Series: | Tropical Medicine and Infectious Disease |
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| Online Access: | https://www.mdpi.com/2414-6366/7/10/274 |
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| author | Ehssan Moglad Samar Saeed Humodi Saeed Hind Ahmed Kwathar Salih Hisham Altayb Wafa Elhag |
| author_facet | Ehssan Moglad Samar Saeed Humodi Saeed Hind Ahmed Kwathar Salih Hisham Altayb Wafa Elhag |
| author_sort | Ehssan Moglad |
| collection | DOAJ |
| description | Background. Pulmonary aspergillosis is a lung infection caused by <i>Aspergillus</i> spp., which can cause severe illnesses in immunocompromised patients with underlying lung disease or who have asthma and inhale their spores. This study aimed to screen the antifungal susceptibility of <i>Aspergillus</i> spp. isolated from patients with underlying pulmonary infections and characterize the isolates using PCR and sequencing. Method. Three hundred and eighty-four sputum or bronchoalveolar lavage samples were collected and processed for the isolation and identification, and characterization of <i>Aspergillus</i> species and molecular amplification of the ITS1-5.8S-ITS2 region by the PCR and Sanger sequencing method. Antifungal susceptibility tests for itraconazole and voriconazole were performed using the E-test. Result. The overall results revealed that out of 384 patients, 32 (8.3%) were positive for fungal growth, including 28 (87.5%) <i>Aspergillus</i> spp. The highest resistance rate (100 and 44.4%) was obtained from itraconazole against <i>A. niger</i> and <i>A. fumigatus</i>. In contrast, voriconazole revealed the best activities against all tested fungi compared to itraconazole. All <i>A. flavus</i> were sensitive to voriconazole, while only 54.5% were sensitive to itraconazole. The MICs of E-test for <i>Aspergillus</i> spp were 1.6 ± 1.8 and 0.6 ± 0.93 for itraconazole and voriconazole, respectively. Conclusions. The prevalence of aspergillosis was high, with a significant association with underlying lung diseases. Voriconazole was the drug of choice for isolated fungi. |
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| series | Tropical Medicine and Infectious Disease |
| spelling | doaj.art-e0971801ddaf450f8040d8c058d56e2c2023-11-24T03:00:10ZengMDPI AGTropical Medicine and Infectious Disease2414-63662022-09-0171027410.3390/tropicalmed7100274Molecular Characterization and Antifungal Susceptibility of <i>Aspergillus</i> spp. among Patients with Underlying Lung DiseasesEhssan Moglad0Samar Saeed1Humodi Saeed2Hind Ahmed3Kwathar Salih4Hisham Altayb5Wafa Elhag6Department of Pharmaceutics, College of Pharmacy, Prince Sattam Bin Abdulaziz University, Al-Kharj P.O. Box 173, Saudi ArabiaDepartment of Microbiology, Faculty of Medical Laboratory Sciences, Sudan University of Science and Technology, Khartoum P.O. Box 407, SudanDepartment of Microbiology, Faculty of Medical Laboratory Sciences, Sudan University of Science and Technology, Khartoum P.O. Box 407, SudanDepartment of Microbiology, Faculty of Medical Laboratory Sciences, Sudan University of Science and Technology, Khartoum P.O. Box 407, SudanDepartment of Microbiology, Faculty of Medical Laboratory Sciences, Sudan University of Science and Technology, Khartoum P.O. Box 407, SudanDepartment of Biochemistry, Faculty of Science, King Abdulaziz University, Building A 90, Jeddah P.O. Box. 80200, Saudi ArabiaDepartment of Basic Medical Sciences, College of Medicine, University of Bisha, Bisha P.O. Box 1290, Saudi ArabiaBackground. Pulmonary aspergillosis is a lung infection caused by <i>Aspergillus</i> spp., which can cause severe illnesses in immunocompromised patients with underlying lung disease or who have asthma and inhale their spores. This study aimed to screen the antifungal susceptibility of <i>Aspergillus</i> spp. isolated from patients with underlying pulmonary infections and characterize the isolates using PCR and sequencing. Method. Three hundred and eighty-four sputum or bronchoalveolar lavage samples were collected and processed for the isolation and identification, and characterization of <i>Aspergillus</i> species and molecular amplification of the ITS1-5.8S-ITS2 region by the PCR and Sanger sequencing method. Antifungal susceptibility tests for itraconazole and voriconazole were performed using the E-test. Result. The overall results revealed that out of 384 patients, 32 (8.3%) were positive for fungal growth, including 28 (87.5%) <i>Aspergillus</i> spp. The highest resistance rate (100 and 44.4%) was obtained from itraconazole against <i>A. niger</i> and <i>A. fumigatus</i>. In contrast, voriconazole revealed the best activities against all tested fungi compared to itraconazole. All <i>A. flavus</i> were sensitive to voriconazole, while only 54.5% were sensitive to itraconazole. The MICs of E-test for <i>Aspergillus</i> spp were 1.6 ± 1.8 and 0.6 ± 0.93 for itraconazole and voriconazole, respectively. Conclusions. The prevalence of aspergillosis was high, with a significant association with underlying lung diseases. Voriconazole was the drug of choice for isolated fungi.https://www.mdpi.com/2414-6366/7/10/274pulmonary aspergillosisantifungalchronic pulmonary infectionPCRKhartoum-Sudan |
| spellingShingle | Ehssan Moglad Samar Saeed Humodi Saeed Hind Ahmed Kwathar Salih Hisham Altayb Wafa Elhag Molecular Characterization and Antifungal Susceptibility of <i>Aspergillus</i> spp. among Patients with Underlying Lung Diseases Tropical Medicine and Infectious Disease pulmonary aspergillosis antifungal chronic pulmonary infection PCR Khartoum-Sudan |
| title | Molecular Characterization and Antifungal Susceptibility of <i>Aspergillus</i> spp. among Patients with Underlying Lung Diseases |
| title_full | Molecular Characterization and Antifungal Susceptibility of <i>Aspergillus</i> spp. among Patients with Underlying Lung Diseases |
| title_fullStr | Molecular Characterization and Antifungal Susceptibility of <i>Aspergillus</i> spp. among Patients with Underlying Lung Diseases |
| title_full_unstemmed | Molecular Characterization and Antifungal Susceptibility of <i>Aspergillus</i> spp. among Patients with Underlying Lung Diseases |
| title_short | Molecular Characterization and Antifungal Susceptibility of <i>Aspergillus</i> spp. among Patients with Underlying Lung Diseases |
| title_sort | molecular characterization and antifungal susceptibility of i aspergillus i spp among patients with underlying lung diseases |
| topic | pulmonary aspergillosis antifungal chronic pulmonary infection PCR Khartoum-Sudan |
| url | https://www.mdpi.com/2414-6366/7/10/274 |
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