Genetic and Ecological Diversity of Escherichia coli and Cryptic Escherichia Clades in Subtropical Aquatic Environments
Escherichia coli not only inhabit the large intestines of human and warm-blooded animals but could also persist in the external environment. However, current knowledge was largely based on host-associated strains. Moreover, cryptic Escherichia clades that were often misidentified as E. coli by conve...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-02-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.811755/full |
| _version_ | 1819274264703926272 |
|---|---|
| author | Xiu Pei Koh Zhiyong Shen Chun Fai Woo Yanping Yu Hau In Lun Sze Wan Cheung Joseph Kai Cho Kwan Stanley Chun Kwan Lau |
| author_facet | Xiu Pei Koh Zhiyong Shen Chun Fai Woo Yanping Yu Hau In Lun Sze Wan Cheung Joseph Kai Cho Kwan Stanley Chun Kwan Lau |
| author_sort | Xiu Pei Koh |
| collection | DOAJ |
| description | Escherichia coli not only inhabit the large intestines of human and warm-blooded animals but could also persist in the external environment. However, current knowledge was largely based on host-associated strains. Moreover, cryptic Escherichia clades that were often misidentified as E. coli by conventional diagnostic methods were discovered. Failure to distinguish them from E. coli sensu stricto could lead to inaccurate conclusions about the population genetics of E. coli. Based on seven housekeeping genes, we determine the genetic and ecological diversity of E. coli and cryptic clades as they occupy aquatic habitats with different characteristics and human impact levels in subtropical Hong Kong. Contrary to previous reports, clade II was the most abundant cryptic lineage co-isolated with E. coli, being especially abundant in relatively pristine subtropical aquatic environments. The phylogenetically distinct cryptic clades and E. coli showed limited recombination and significant genetic divergence. Analyses indicated that these clade II strains were ecologically differentiated from typical E. coli; some may even represent novel environmental Escherichia clades that were closely related to the original clade II strains of fecal origins. E. coli of diverse origins exhibited clonality amidst divergent genotypes STs, echoing other studies in that recombination in housekeeping genes was insufficient to disrupt phylogenetic signals of the largely clonal E. coli. Notably, environmental E. coli were less diverse than fecal isolates despite contributing many new alleles and STs. Finally, we demonstrated that human activities influenced the distribution of E. coli and clade II in a small aquatic continuum. Moving from relatively pristine sites toward areas with higher human disturbance, the abundance of clade II isolates and new E. coli genotypes reduces, while E. coli bearing class I integrons and belonging to CCs of public health concern accumulates. Altogether, this work revealed the new genetic diversity of E. coli and cryptic clades embedded in selected subtropical aquatic habitats, especially relatively pristine sites, which will aid a more thorough understanding of the extent of their genetic and functional variations in relation to diverse habitats with varied conditions. |
| first_indexed | 2024-12-23T23:05:40Z |
| format | Article |
| id | doaj.art-e0982728604648e38ea6ef16946c311a |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-23T23:05:40Z |
| publishDate | 2022-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-e0982728604648e38ea6ef16946c311a2022-12-21T17:26:49ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-02-011310.3389/fmicb.2022.811755811755Genetic and Ecological Diversity of Escherichia coli and Cryptic Escherichia Clades in Subtropical Aquatic EnvironmentsXiu Pei Koh0Zhiyong Shen1Chun Fai Woo2Yanping Yu3Hau In Lun4Sze Wan Cheung5Joseph Kai Cho Kwan6Stanley Chun Kwan Lau7Division of Environment and Sustainability, The Hong Kong University of Science and Technology, Kowloon, Hong Kong SAR, ChinaDepartment of Ocean Science, The Hong Kong University of Science and Technology, Kowloon, Hong Kong SAR, ChinaDivision of Environment and Sustainability, The Hong Kong University of Science and Technology, Kowloon, Hong Kong SAR, ChinaDepartment of Ocean Science, The Hong Kong University of Science and Technology, Kowloon, Hong Kong SAR, ChinaDepartment of Ocean Science, The Hong Kong University of Science and Technology, Kowloon, Hong Kong SAR, ChinaDivision of Life Science, The Hong Kong University of Science and Technology, Hong Kong, Hong Kong SAR, ChinaDivision of Environment and Sustainability, The Hong Kong University of Science and Technology, Kowloon, Hong Kong SAR, ChinaDepartment of Ocean Science, The Hong Kong University of Science and Technology, Kowloon, Hong Kong SAR, ChinaEscherichia coli not only inhabit the large intestines of human and warm-blooded animals but could also persist in the external environment. However, current knowledge was largely based on host-associated strains. Moreover, cryptic Escherichia clades that were often misidentified as E. coli by conventional diagnostic methods were discovered. Failure to distinguish them from E. coli sensu stricto could lead to inaccurate conclusions about the population genetics of E. coli. Based on seven housekeeping genes, we determine the genetic and ecological diversity of E. coli and cryptic clades as they occupy aquatic habitats with different characteristics and human impact levels in subtropical Hong Kong. Contrary to previous reports, clade II was the most abundant cryptic lineage co-isolated with E. coli, being especially abundant in relatively pristine subtropical aquatic environments. The phylogenetically distinct cryptic clades and E. coli showed limited recombination and significant genetic divergence. Analyses indicated that these clade II strains were ecologically differentiated from typical E. coli; some may even represent novel environmental Escherichia clades that were closely related to the original clade II strains of fecal origins. E. coli of diverse origins exhibited clonality amidst divergent genotypes STs, echoing other studies in that recombination in housekeeping genes was insufficient to disrupt phylogenetic signals of the largely clonal E. coli. Notably, environmental E. coli were less diverse than fecal isolates despite contributing many new alleles and STs. Finally, we demonstrated that human activities influenced the distribution of E. coli and clade II in a small aquatic continuum. Moving from relatively pristine sites toward areas with higher human disturbance, the abundance of clade II isolates and new E. coli genotypes reduces, while E. coli bearing class I integrons and belonging to CCs of public health concern accumulates. Altogether, this work revealed the new genetic diversity of E. coli and cryptic clades embedded in selected subtropical aquatic habitats, especially relatively pristine sites, which will aid a more thorough understanding of the extent of their genetic and functional variations in relation to diverse habitats with varied conditions.https://www.frontiersin.org/articles/10.3389/fmicb.2022.811755/fullEscherichiagenetic diversitysubtropicalaquatic environmentcryptic cladesenvironmental E. coli |
| spellingShingle | Xiu Pei Koh Zhiyong Shen Chun Fai Woo Yanping Yu Hau In Lun Sze Wan Cheung Joseph Kai Cho Kwan Stanley Chun Kwan Lau Genetic and Ecological Diversity of Escherichia coli and Cryptic Escherichia Clades in Subtropical Aquatic Environments Frontiers in Microbiology Escherichia genetic diversity subtropical aquatic environment cryptic clades environmental E. coli |
| title | Genetic and Ecological Diversity of Escherichia coli and Cryptic Escherichia Clades in Subtropical Aquatic Environments |
| title_full | Genetic and Ecological Diversity of Escherichia coli and Cryptic Escherichia Clades in Subtropical Aquatic Environments |
| title_fullStr | Genetic and Ecological Diversity of Escherichia coli and Cryptic Escherichia Clades in Subtropical Aquatic Environments |
| title_full_unstemmed | Genetic and Ecological Diversity of Escherichia coli and Cryptic Escherichia Clades in Subtropical Aquatic Environments |
| title_short | Genetic and Ecological Diversity of Escherichia coli and Cryptic Escherichia Clades in Subtropical Aquatic Environments |
| title_sort | genetic and ecological diversity of escherichia coli and cryptic escherichia clades in subtropical aquatic environments |
| topic | Escherichia genetic diversity subtropical aquatic environment cryptic clades environmental E. coli |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.811755/full |
| work_keys_str_mv | AT xiupeikoh geneticandecologicaldiversityofescherichiacoliandcrypticescherichiacladesinsubtropicalaquaticenvironments AT zhiyongshen geneticandecologicaldiversityofescherichiacoliandcrypticescherichiacladesinsubtropicalaquaticenvironments AT chunfaiwoo geneticandecologicaldiversityofescherichiacoliandcrypticescherichiacladesinsubtropicalaquaticenvironments AT yanpingyu geneticandecologicaldiversityofescherichiacoliandcrypticescherichiacladesinsubtropicalaquaticenvironments AT hauinlun geneticandecologicaldiversityofescherichiacoliandcrypticescherichiacladesinsubtropicalaquaticenvironments AT szewancheung geneticandecologicaldiversityofescherichiacoliandcrypticescherichiacladesinsubtropicalaquaticenvironments AT josephkaichokwan geneticandecologicaldiversityofescherichiacoliandcrypticescherichiacladesinsubtropicalaquaticenvironments AT stanleychunkwanlau geneticandecologicaldiversityofescherichiacoliandcrypticescherichiacladesinsubtropicalaquaticenvironments |