<i>Leptospira</i> Survey in Wild Boar (<i>Sus scrofa</i>) Hunted in Tuscany, Central Italy
Leptospirosis is a re-emerging, worldwide zoonosis, and wild boar (<i>Sus scrofa</i>) are involved in its epidemiology as the reservoir. The aim of this study was to investigate the prevalence of <i>Leptospira</i> with serological, bacteriological, and molecular assays in wil...
| Những tác giả chính: | , , , , |
|---|---|
| Định dạng: | Bài viết |
| Ngôn ngữ: | English |
| Được phát hành: |
MDPI AG
2020-05-01
|
| Loạt: | Pathogens |
| Những chủ đề: | |
| Truy cập trực tuyến: | https://www.mdpi.com/2076-0817/9/5/377 |
| _version_ | 1827716885500657664 |
|---|---|
| author | Giovanni Cilia Fabrizio Bertelloni Marta Angelini Domenico Cerri Filippo Fratini |
| author_facet | Giovanni Cilia Fabrizio Bertelloni Marta Angelini Domenico Cerri Filippo Fratini |
| author_sort | Giovanni Cilia |
| collection | DOAJ |
| description | Leptospirosis is a re-emerging, worldwide zoonosis, and wild boar (<i>Sus scrofa</i>) are involved in its epidemiology as the reservoir. The aim of this study was to investigate the prevalence of <i>Leptospira</i> with serological, bacteriological, and molecular assays in wild boar hunted in Tuscany (Italy) during two hunting seasons. In total, 287 specimens of sera, kidneys, and liver were collected to perform microscopic agglutination tests (MATs), isolation, and RealTime PCR to detect pathogenic (<i>lipL32</i> gene), intermediate (<i>16S rRNA</i> gene), and saprophytic (<i>23S rRNA</i> gene) <i>Leptospira</i>. Within sera, 39 (13.59%) were positive to the MAT, and Australis was the most represented serogroup (4.88%), followed by Pomona (4.18%), and Tarassovi (3.14%). Moreover, four <i>Leptospira</i> cultures were positive, and once isolates were identified, one was identified as <i>L. borgpetersenii</i> serovar Tarassovi, and three as <i>L. interrogans</i> serovar Bratislava. Pathogenic <i>Leptospira</i> DNA were detected in 32 wild boar kidneys (11.15%). The characterization through the amplification of the <i>rrs2</i> gene highlighted their belonging to <i>L. interrogans</i> (23 kidneys), <i>L. borgpetersenii</i> (four), and <i>L. kirschneri</i> (one), while nine kidneys (3.14%) were positive for intermediate <i>Leptospira</i>, all belonging to <i>L. fainei.</i> The results of this study confirmed the importance of wild boar in the epidemiology of leptospirosis among wildlife in Central Italy. |
| first_indexed | 2024-03-10T19:50:03Z |
| format | Article |
| id | doaj.art-e09b51d0a09b4768844d21c03cefde7d |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-10T19:50:03Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-e09b51d0a09b4768844d21c03cefde7d2023-11-20T00:27:47ZengMDPI AGPathogens2076-08172020-05-019537710.3390/pathogens9050377<i>Leptospira</i> Survey in Wild Boar (<i>Sus scrofa</i>) Hunted in Tuscany, Central ItalyGiovanni Cilia0Fabrizio Bertelloni1Marta Angelini2Domenico Cerri3Filippo Fratini4Department of Veterinary Sciences, University of Pisa, Viale delle Piagge 2, 56124 Pisa, ItalyDepartment of Veterinary Sciences, University of Pisa, Viale delle Piagge 2, 56124 Pisa, ItalyDepartment of Veterinary Sciences, University of Pisa, Viale delle Piagge 2, 56124 Pisa, ItalyDepartment of Veterinary Sciences, University of Pisa, Viale delle Piagge 2, 56124 Pisa, ItalyDepartment of Veterinary Sciences, University of Pisa, Viale delle Piagge 2, 56124 Pisa, ItalyLeptospirosis is a re-emerging, worldwide zoonosis, and wild boar (<i>Sus scrofa</i>) are involved in its epidemiology as the reservoir. The aim of this study was to investigate the prevalence of <i>Leptospira</i> with serological, bacteriological, and molecular assays in wild boar hunted in Tuscany (Italy) during two hunting seasons. In total, 287 specimens of sera, kidneys, and liver were collected to perform microscopic agglutination tests (MATs), isolation, and RealTime PCR to detect pathogenic (<i>lipL32</i> gene), intermediate (<i>16S rRNA</i> gene), and saprophytic (<i>23S rRNA</i> gene) <i>Leptospira</i>. Within sera, 39 (13.59%) were positive to the MAT, and Australis was the most represented serogroup (4.88%), followed by Pomona (4.18%), and Tarassovi (3.14%). Moreover, four <i>Leptospira</i> cultures were positive, and once isolates were identified, one was identified as <i>L. borgpetersenii</i> serovar Tarassovi, and three as <i>L. interrogans</i> serovar Bratislava. Pathogenic <i>Leptospira</i> DNA were detected in 32 wild boar kidneys (11.15%). The characterization through the amplification of the <i>rrs2</i> gene highlighted their belonging to <i>L. interrogans</i> (23 kidneys), <i>L. borgpetersenii</i> (four), and <i>L. kirschneri</i> (one), while nine kidneys (3.14%) were positive for intermediate <i>Leptospira</i>, all belonging to <i>L. fainei.</i> The results of this study confirmed the importance of wild boar in the epidemiology of leptospirosis among wildlife in Central Italy.https://www.mdpi.com/2076-0817/9/5/377leptospirosiszoonosisinfectious diseasemultilocus sequence typing (MLST)wildlife<i>Leptospira fainei</i> |
| spellingShingle | Giovanni Cilia Fabrizio Bertelloni Marta Angelini Domenico Cerri Filippo Fratini <i>Leptospira</i> Survey in Wild Boar (<i>Sus scrofa</i>) Hunted in Tuscany, Central Italy Pathogens leptospirosis zoonosis infectious disease multilocus sequence typing (MLST) wildlife <i>Leptospira fainei</i> |
| title | <i>Leptospira</i> Survey in Wild Boar (<i>Sus scrofa</i>) Hunted in Tuscany, Central Italy |
| title_full | <i>Leptospira</i> Survey in Wild Boar (<i>Sus scrofa</i>) Hunted in Tuscany, Central Italy |
| title_fullStr | <i>Leptospira</i> Survey in Wild Boar (<i>Sus scrofa</i>) Hunted in Tuscany, Central Italy |
| title_full_unstemmed | <i>Leptospira</i> Survey in Wild Boar (<i>Sus scrofa</i>) Hunted in Tuscany, Central Italy |
| title_short | <i>Leptospira</i> Survey in Wild Boar (<i>Sus scrofa</i>) Hunted in Tuscany, Central Italy |
| title_sort | i leptospira i survey in wild boar i sus scrofa i hunted in tuscany central italy |
| topic | leptospirosis zoonosis infectious disease multilocus sequence typing (MLST) wildlife <i>Leptospira fainei</i> |
| url | https://www.mdpi.com/2076-0817/9/5/377 |
| work_keys_str_mv | AT giovannicilia ileptospiraisurveyinwildboarisusscrofaihuntedintuscanycentralitaly AT fabriziobertelloni ileptospiraisurveyinwildboarisusscrofaihuntedintuscanycentralitaly AT martaangelini ileptospiraisurveyinwildboarisusscrofaihuntedintuscanycentralitaly AT domenicocerri ileptospiraisurveyinwildboarisusscrofaihuntedintuscanycentralitaly AT filippofratini ileptospiraisurveyinwildboarisusscrofaihuntedintuscanycentralitaly |