High Prevalence of Rotavirus A in Raw Sewage Samples from Northeast Spain
Rotavirus A (RVA) is the most common virus associated with infantile gastroenteritis worldwide, being a public health threat, as it is excreted in large amounts in stool and can persist in the environment for extended periods. In this study, we performed the detection of RVA and human adenovirus (HA...
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MDPI AG
2020-03-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/12/3/318 |
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| author | Marcelle Silva-Sales Sandra Martínez-Puchol Eloy Gonzales-Gustavson Ayalkibet Hundesa Rosina Gironès |
| author_facet | Marcelle Silva-Sales Sandra Martínez-Puchol Eloy Gonzales-Gustavson Ayalkibet Hundesa Rosina Gironès |
| author_sort | Marcelle Silva-Sales |
| collection | DOAJ |
| description | Rotavirus A (RVA) is the most common virus associated with infantile gastroenteritis worldwide, being a public health threat, as it is excreted in large amounts in stool and can persist in the environment for extended periods. In this study, we performed the detection of RVA and human adenovirus (HAdV) by TaqMan qPCR and assessed the circulation of RVA genotypes in three wastewater treatment plants (WWTPs) between 2015 and 2016 in Catalonia, Spain. RVA was detected in 90% and HAdV in 100% of the WWTP samples, with viral loads ranging between 3.96 × 10<sup>4</sup> and 3.30 × 10<sup>8</sup> RT-PCR Units/L and 9.51 × 10<sup>4</sup> and 1.16 × 10<sup>6</sup> genomic copies/L, respectively. RVA VP7 and VP4 gene analysis revealed the circulation of G2, G3, G9, G12, P[4], P[8], P[9] and P[10]. Nucleotide sequencing (VP6 fragment) showed the circulation of I1 and I2 genotypes, commonly associated with human, bovine and porcine strains. It is important to mention that the RVA strains isolated from the WWTPs were different from those recovered from piglets and calves living in the same area of single sampling in 2016. These data highlight the importance of monitoring water matrices for RVA epidemiology and may be a useful tool to evaluate and predict possible emergence/reemergence of uncommon strains in a region. |
| first_indexed | 2024-04-12T07:51:44Z |
| format | Article |
| id | doaj.art-e09b623cc1be4a4b9b1bd00dce8b50a5 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-04-12T07:51:44Z |
| publishDate | 2020-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-e09b623cc1be4a4b9b1bd00dce8b50a52022-12-22T03:41:35ZengMDPI AGViruses1999-49152020-03-0112331810.3390/v12030318v12030318High Prevalence of Rotavirus A in Raw Sewage Samples from Northeast SpainMarcelle Silva-Sales0Sandra Martínez-Puchol1Eloy Gonzales-Gustavson2Ayalkibet Hundesa3Rosina Gironès4Microbiology, Virology and Biotechnology Section, Department of Genetics, Microbiology and Statistics, Faculty of Biology, University of Barcelona, Av. Diagonal 643, Barcelona 08028, SpainMicrobiology, Virology and Biotechnology Section, Department of Genetics, Microbiology and Statistics, Faculty of Biology, University of Barcelona, Av. Diagonal 643, Barcelona 08028, SpainMicrobiology, Virology and Biotechnology Section, Department of Genetics, Microbiology and Statistics, Faculty of Biology, University of Barcelona, Av. Diagonal 643, Barcelona 08028, SpainMicrobiology, Virology and Biotechnology Section, Department of Genetics, Microbiology and Statistics, Faculty of Biology, University of Barcelona, Av. Diagonal 643, Barcelona 08028, SpainMicrobiology, Virology and Biotechnology Section, Department of Genetics, Microbiology and Statistics, Faculty of Biology, University of Barcelona, Av. Diagonal 643, Barcelona 08028, SpainRotavirus A (RVA) is the most common virus associated with infantile gastroenteritis worldwide, being a public health threat, as it is excreted in large amounts in stool and can persist in the environment for extended periods. In this study, we performed the detection of RVA and human adenovirus (HAdV) by TaqMan qPCR and assessed the circulation of RVA genotypes in three wastewater treatment plants (WWTPs) between 2015 and 2016 in Catalonia, Spain. RVA was detected in 90% and HAdV in 100% of the WWTP samples, with viral loads ranging between 3.96 × 10<sup>4</sup> and 3.30 × 10<sup>8</sup> RT-PCR Units/L and 9.51 × 10<sup>4</sup> and 1.16 × 10<sup>6</sup> genomic copies/L, respectively. RVA VP7 and VP4 gene analysis revealed the circulation of G2, G3, G9, G12, P[4], P[8], P[9] and P[10]. Nucleotide sequencing (VP6 fragment) showed the circulation of I1 and I2 genotypes, commonly associated with human, bovine and porcine strains. It is important to mention that the RVA strains isolated from the WWTPs were different from those recovered from piglets and calves living in the same area of single sampling in 2016. These data highlight the importance of monitoring water matrices for RVA epidemiology and may be a useful tool to evaluate and predict possible emergence/reemergence of uncommon strains in a region.https://www.mdpi.com/1999-4915/12/3/318rotavirus awastewaterviral quantificationmolecular characterization |
| spellingShingle | Marcelle Silva-Sales Sandra Martínez-Puchol Eloy Gonzales-Gustavson Ayalkibet Hundesa Rosina Gironès High Prevalence of Rotavirus A in Raw Sewage Samples from Northeast Spain Viruses rotavirus a wastewater viral quantification molecular characterization |
| title | High Prevalence of Rotavirus A in Raw Sewage Samples from Northeast Spain |
| title_full | High Prevalence of Rotavirus A in Raw Sewage Samples from Northeast Spain |
| title_fullStr | High Prevalence of Rotavirus A in Raw Sewage Samples from Northeast Spain |
| title_full_unstemmed | High Prevalence of Rotavirus A in Raw Sewage Samples from Northeast Spain |
| title_short | High Prevalence of Rotavirus A in Raw Sewage Samples from Northeast Spain |
| title_sort | high prevalence of rotavirus a in raw sewage samples from northeast spain |
| topic | rotavirus a wastewater viral quantification molecular characterization |
| url | https://www.mdpi.com/1999-4915/12/3/318 |
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