Gastric cancer‐associated long non‐coding RNA profiling and noninvasive biomarker screening based on a high‐risk population cohort
Abstract Background Effective noninvasive biomarkers of gastric cancer (GC) are critical for early detection and improvement of prognosis. We performed genome‐wide long non‐coding RNA (lncRNA) microarray analysis to identify and validate novel GC biomarkers depending on a high‐risk population cohort...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-06-01
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| Series: | Cancer Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/cam4.5905 |
| _version_ | 1797800259561193472 |
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| author | Xiaoying Guo Yang Zhang Zhiyi Zhang Linzhi Lu Yuqin Liu Zhexuan Li Tong Zhou Jingying Zhang Wenqing Li Weicheng You Guoquan Tao Wanqing Chen Hongmei Zeng Kaifeng Pan |
| author_facet | Xiaoying Guo Yang Zhang Zhiyi Zhang Linzhi Lu Yuqin Liu Zhexuan Li Tong Zhou Jingying Zhang Wenqing Li Weicheng You Guoquan Tao Wanqing Chen Hongmei Zeng Kaifeng Pan |
| author_sort | Xiaoying Guo |
| collection | DOAJ |
| description | Abstract Background Effective noninvasive biomarkers of gastric cancer (GC) are critical for early detection and improvement of prognosis. We performed genome‐wide long non‐coding RNA (lncRNA) microarray analysis to identify and validate novel GC biomarkers depending on a high‐risk population cohort. Methods LncRNA profiles were described using the Human LncRNA Microarray between GC and control plasma samples. The differential candidate lncRNAs were validated in two stages by quantitative reverse transcription polymerase chain reaction (qRT‐PCR). We further evaluated the joint effect between the GC‐associated lncRNA and Helicobacter pylori (H. pylori) infection on the risk of cardia and non‐cardia GC, respectively. Results Different lncRNA expression profiles were identified between GC and control plasma with a total of 1206 differential lncRNAs including 470 upregulated and 736 downregulated in GC compared with the control group. The eight significantly upregulated lncRNAs (RP11‐521D12.1, AC011995.3, RP11‐5P4.3, RP11‐244 K5.6, RP11‐422 J15.1, CTD‐2306 M5.1, CTC‐428G20.2, and AC009133.20) in GC cases both in the present study and a similar microarray screening study by our collaborative team were selected for a two‐stage validation. After the large sample size validation, the subjects with higher expression of RP11‐244 K5.6 showed a significantly increased risk of GC with an adjusted odds ratio (OR) as 2.68 and 95% confidence interval (CI) as 1.15–6.24. Joint effects between RP11‐244 K5.6 expression and H. pylori infection on the risk of GC were evaluated with no statistical significance. Conclusions Our study found different lncRNA expression profiles between GC and control plasma and preliminarily identified RP11‐244 K5.6 as a potential noninvasive biomarker for GC screening. |
| first_indexed | 2024-03-13T04:32:28Z |
| format | Article |
| id | doaj.art-e09b8a768ee34d03a2dd3425fa2330d6 |
| institution | Directory Open Access Journal |
| issn | 2045-7634 |
| language | English |
| last_indexed | 2024-03-13T04:32:28Z |
| publishDate | 2023-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | Cancer Medicine |
| spelling | doaj.art-e09b8a768ee34d03a2dd3425fa2330d62023-06-19T12:03:52ZengWileyCancer Medicine2045-76342023-06-011211127281273810.1002/cam4.5905Gastric cancer‐associated long non‐coding RNA profiling and noninvasive biomarker screening based on a high‐risk population cohortXiaoying Guo0Yang Zhang1Zhiyi Zhang2Linzhi Lu3Yuqin Liu4Zhexuan Li5Tong Zhou6Jingying Zhang7Wenqing Li8Weicheng You9Guoquan Tao10Wanqing Chen11Hongmei Zeng12Kaifeng Pan13Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Department of Cancer Epidemiology, Peking University School of Oncology Peking University Cancer Hospital & Institute Beijing ChinaKey Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Department of Cancer Epidemiology, Peking University School of Oncology Peking University Cancer Hospital & Institute Beijing ChinaDepartment of Gastroenterology Gansu Wuwei Tumor Hospital Wuwei ChinaDepartment of Gastroenterology Gansu Wuwei Tumor Hospital Wuwei ChinaCancer Epidemiology Research Center Gansu Provincial Cancer Hospital Lanzhou ChinaKey Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Department of Cancer Epidemiology, Peking University School of Oncology Peking University Cancer Hospital & Institute Beijing ChinaKey Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Department of Cancer Epidemiology, Peking University School of Oncology Peking University Cancer Hospital & Institute Beijing ChinaKey Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Department of Cancer Epidemiology, Peking University School of Oncology Peking University Cancer Hospital & Institute Beijing ChinaKey Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Department of Cancer Epidemiology, Peking University School of Oncology Peking University Cancer Hospital & Institute Beijing ChinaKey Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Department of Cancer Epidemiology, Peking University School of Oncology Peking University Cancer Hospital & Institute Beijing ChinaDepartment of General Surgery, The Affiliated Huai'an No.1 People's Hospital Nanjing Medical University Huai'an 223300 ChinaNational Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital Chinese Academy of Medical Sciences and Peking Union Medical College Beijing ChinaNational Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital Chinese Academy of Medical Sciences and Peking Union Medical College Beijing ChinaKey Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Department of Cancer Epidemiology, Peking University School of Oncology Peking University Cancer Hospital & Institute Beijing ChinaAbstract Background Effective noninvasive biomarkers of gastric cancer (GC) are critical for early detection and improvement of prognosis. We performed genome‐wide long non‐coding RNA (lncRNA) microarray analysis to identify and validate novel GC biomarkers depending on a high‐risk population cohort. Methods LncRNA profiles were described using the Human LncRNA Microarray between GC and control plasma samples. The differential candidate lncRNAs were validated in two stages by quantitative reverse transcription polymerase chain reaction (qRT‐PCR). We further evaluated the joint effect between the GC‐associated lncRNA and Helicobacter pylori (H. pylori) infection on the risk of cardia and non‐cardia GC, respectively. Results Different lncRNA expression profiles were identified between GC and control plasma with a total of 1206 differential lncRNAs including 470 upregulated and 736 downregulated in GC compared with the control group. The eight significantly upregulated lncRNAs (RP11‐521D12.1, AC011995.3, RP11‐5P4.3, RP11‐244 K5.6, RP11‐422 J15.1, CTD‐2306 M5.1, CTC‐428G20.2, and AC009133.20) in GC cases both in the present study and a similar microarray screening study by our collaborative team were selected for a two‐stage validation. After the large sample size validation, the subjects with higher expression of RP11‐244 K5.6 showed a significantly increased risk of GC with an adjusted odds ratio (OR) as 2.68 and 95% confidence interval (CI) as 1.15–6.24. Joint effects between RP11‐244 K5.6 expression and H. pylori infection on the risk of GC were evaluated with no statistical significance. Conclusions Our study found different lncRNA expression profiles between GC and control plasma and preliminarily identified RP11‐244 K5.6 as a potential noninvasive biomarker for GC screening.https://doi.org/10.1002/cam4.5905biomarkersgastric cancerLncRNAsmicroarrayplasma |
| spellingShingle | Xiaoying Guo Yang Zhang Zhiyi Zhang Linzhi Lu Yuqin Liu Zhexuan Li Tong Zhou Jingying Zhang Wenqing Li Weicheng You Guoquan Tao Wanqing Chen Hongmei Zeng Kaifeng Pan Gastric cancer‐associated long non‐coding RNA profiling and noninvasive biomarker screening based on a high‐risk population cohort Cancer Medicine biomarkers gastric cancer LncRNAs microarray plasma |
| title | Gastric cancer‐associated long non‐coding RNA profiling and noninvasive biomarker screening based on a high‐risk population cohort |
| title_full | Gastric cancer‐associated long non‐coding RNA profiling and noninvasive biomarker screening based on a high‐risk population cohort |
| title_fullStr | Gastric cancer‐associated long non‐coding RNA profiling and noninvasive biomarker screening based on a high‐risk population cohort |
| title_full_unstemmed | Gastric cancer‐associated long non‐coding RNA profiling and noninvasive biomarker screening based on a high‐risk population cohort |
| title_short | Gastric cancer‐associated long non‐coding RNA profiling and noninvasive biomarker screening based on a high‐risk population cohort |
| title_sort | gastric cancer associated long non coding rna profiling and noninvasive biomarker screening based on a high risk population cohort |
| topic | biomarkers gastric cancer LncRNAs microarray plasma |
| url | https://doi.org/10.1002/cam4.5905 |
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