Impacts of Different Prenatal Supplementation Strategies on the Plasma Metabolome of Bulls in the Rearing and Finishing Phase
This study investigated the effects of maternal nutrition on the plasma metabolome of Nellore bulls in the rearing and finishing phases, and metabolic differences between these phases. For this study, three nutritional approaches were used in 126 cows during pregnancy: NP—(control) mineral supplemen...
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MDPI AG
2023-02-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/13/2/259 |
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| author | Guilherme Henrique Gebim Polizel Arícia Christofaro Fernandes Édison Furlan Barbara Carolina Teixeira Prati José Bento Sterman Ferraz Miguel Henrique de Almeida Santana |
| author_facet | Guilherme Henrique Gebim Polizel Arícia Christofaro Fernandes Édison Furlan Barbara Carolina Teixeira Prati José Bento Sterman Ferraz Miguel Henrique de Almeida Santana |
| author_sort | Guilherme Henrique Gebim Polizel |
| collection | DOAJ |
| description | This study investigated the effects of maternal nutrition on the plasma metabolome of Nellore bulls in the rearing and finishing phases, and metabolic differences between these phases. For this study, three nutritional approaches were used in 126 cows during pregnancy: NP—(control) mineral supplementation; PP—protein-energy supplementation in the final third; and FP—protein-energy supplementation during the entire pregnancy. We collected blood samples from male offspring in the rearing (450 ± 28 days old) and finishing phases (660 ± 28 days old). The blood was processed, and from plasma samples, we performed the targeted metabolome analysis (AbsoluteIDQ<sup>®</sup> p180 Kit). Multiple linear regression, principal component analysis (PCA), repeated measures analysis over time, and an enrichment analysis were performed. PCA showed an overlap of treatments and time clusters in the analyses. We identified significant metabolites among the treatments (rearing phase = six metabolites; finishing phase = three metabolites) and over time (21 metabolites). No significant metabolic pathways were found in the finishing phase, however, we found significant pathways in the rearing phase (Arginine biosynthesis and Histidine metabolism). Thus, prenatal nutrition impacted on plasma metabolome of bulls during the rearing and finishing phase and the different production stages showed an effect on the metabolic levels of bulls. |
| first_indexed | 2024-03-11T08:26:43Z |
| format | Article |
| id | doaj.art-e09bbd8bc6f443478e18e45fd9edc1b7 |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-11T08:26:43Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-e09bbd8bc6f443478e18e45fd9edc1b72023-11-16T22:05:06ZengMDPI AGMetabolites2218-19892023-02-0113225910.3390/metabo13020259Impacts of Different Prenatal Supplementation Strategies on the Plasma Metabolome of Bulls in the Rearing and Finishing PhaseGuilherme Henrique Gebim Polizel0Arícia Christofaro Fernandes1Édison Furlan2Barbara Carolina Teixeira Prati3José Bento Sterman Ferraz4Miguel Henrique de Almeida Santana5Department of Animal Science, Faculty of Animal Science and Food Engineering—USP, Av. Duque de Caxias Norte, 225, Pirassununga 13635-900, SP, BrazilDepartment of Animal Science, Faculty of Animal Science and Food Engineering—USP, Av. Duque de Caxias Norte, 225, Pirassununga 13635-900, SP, BrazilDepartment of Animal Science, Faculty of Animal Science and Food Engineering—USP, Av. Duque de Caxias Norte, 225, Pirassununga 13635-900, SP, BrazilDepartment of Animal Science, Faculty of Animal Science and Food Engineering—USP, Av. Duque de Caxias Norte, 225, Pirassununga 13635-900, SP, BrazilDepartment of Basic Sciences, Faculty of Animal Science and Food Engineering—USP, Av. Duque de Caxias Norte, 225, Pirassununga 13635-900, SP, BrazilDepartment of Animal Science, Faculty of Animal Science and Food Engineering—USP, Av. Duque de Caxias Norte, 225, Pirassununga 13635-900, SP, BrazilThis study investigated the effects of maternal nutrition on the plasma metabolome of Nellore bulls in the rearing and finishing phases, and metabolic differences between these phases. For this study, three nutritional approaches were used in 126 cows during pregnancy: NP—(control) mineral supplementation; PP—protein-energy supplementation in the final third; and FP—protein-energy supplementation during the entire pregnancy. We collected blood samples from male offspring in the rearing (450 ± 28 days old) and finishing phases (660 ± 28 days old). The blood was processed, and from plasma samples, we performed the targeted metabolome analysis (AbsoluteIDQ<sup>®</sup> p180 Kit). Multiple linear regression, principal component analysis (PCA), repeated measures analysis over time, and an enrichment analysis were performed. PCA showed an overlap of treatments and time clusters in the analyses. We identified significant metabolites among the treatments (rearing phase = six metabolites; finishing phase = three metabolites) and over time (21 metabolites). No significant metabolic pathways were found in the finishing phase, however, we found significant pathways in the rearing phase (Arginine biosynthesis and Histidine metabolism). Thus, prenatal nutrition impacted on plasma metabolome of bulls during the rearing and finishing phase and the different production stages showed an effect on the metabolic levels of bulls.https://www.mdpi.com/2218-1989/13/2/259beef cattlefetal programmingmetabolismmetabolomicsnutrigenomics |
| spellingShingle | Guilherme Henrique Gebim Polizel Arícia Christofaro Fernandes Édison Furlan Barbara Carolina Teixeira Prati José Bento Sterman Ferraz Miguel Henrique de Almeida Santana Impacts of Different Prenatal Supplementation Strategies on the Plasma Metabolome of Bulls in the Rearing and Finishing Phase Metabolites beef cattle fetal programming metabolism metabolomics nutrigenomics |
| title | Impacts of Different Prenatal Supplementation Strategies on the Plasma Metabolome of Bulls in the Rearing and Finishing Phase |
| title_full | Impacts of Different Prenatal Supplementation Strategies on the Plasma Metabolome of Bulls in the Rearing and Finishing Phase |
| title_fullStr | Impacts of Different Prenatal Supplementation Strategies on the Plasma Metabolome of Bulls in the Rearing and Finishing Phase |
| title_full_unstemmed | Impacts of Different Prenatal Supplementation Strategies on the Plasma Metabolome of Bulls in the Rearing and Finishing Phase |
| title_short | Impacts of Different Prenatal Supplementation Strategies on the Plasma Metabolome of Bulls in the Rearing and Finishing Phase |
| title_sort | impacts of different prenatal supplementation strategies on the plasma metabolome of bulls in the rearing and finishing phase |
| topic | beef cattle fetal programming metabolism metabolomics nutrigenomics |
| url | https://www.mdpi.com/2218-1989/13/2/259 |
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