Effect of garlic oil nanoemulsion against multidrug resistant Pseudomonas aeruginosa isolated from broiler
This work aimed to focus on the antibacterial properties of garlic nanoemulsion on some multidrug resistance (MDR) strains of<em> Pseudomona</em>s <em>a</em><em>eruginos</em>aisolated from broiler farms and hatcheries in Sharkia and Ismailia governorates, Egypt. &...
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| Format: | Article |
| Language: | Arabic |
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University of Mosul, College of Veterinary Medicine
2022-10-01
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| Series: | Iraqi Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | https://www.vetmedmosul.com/article_175416_e3d277c35a135887c219358336468878.pdf |
| _version_ | 1797996313693913088 |
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| author | Amal S. El-Oksh Dalia M. Elmasry Ghada A. Ibrahim |
| author_facet | Amal S. El-Oksh Dalia M. Elmasry Ghada A. Ibrahim |
| author_sort | Amal S. El-Oksh |
| collection | DOAJ |
| description | This work aimed to focus on the antibacterial properties of garlic nanoemulsion on some multidrug resistance (MDR) strains of<em> Pseudomona</em>s <em>a</em><em>eruginos</em>aisolated from broiler farms and hatcheries in Sharkia and Ismailia governorates, Egypt. <em>Pseudomonas</em> spp. was isolated in 21.3% of collected samples. It was isolated from younger broilers 1-10 days with an incidence rate of 22% (11/50), older broilers 16% (8/50), dead embryo in shell 31.4% (11/35), and from hatcheries was 13.3% (2/15). There was a variable range of antibiotic resistance ranging from 66.7-100% against the isolated strains of <em>P. aeruginosa</em>. Tetracycline and sulfamethoxazole-trimethoprim achieved the highest resistance rates, while penicillin and gentamycin were of a lower rate. However, norfloxacin, ciprofloxacin, and colistin were the most sensitive antibiotics against examined MDR <em>P. aeruginosa.</em>16SrDNA gene was found in ten <em>P. aeruginosa</em> isolates. Theseisolates were found to be virulent as <em>oprL</em> gene was detected in all isolates 100%. In addition, <em>tetA(A),bla</em>TEM<em>,arr, </em>and<em> mex</em>Rantibiotic resistance genes were shown positive 100% in all MDR <em>P. aeruginosa</em> isolates. Minimum Inhibitory Concentration (MIC) values showed that garlic nanoemulsion (GN) was effective against examined <em>P. aeruginosa</em> at different concentrations. GN had 29.61% sulfur compounds of active components with 0.52 ug/ml of IC<sub>50</sub> and 40.94 nm size with polydispersity index: 0.165 using dynamic light scattering had a 19.6± 5.11mV. In conclusion, the application of garlic nanoemulsion is an excellent alternative candidate to antibiotics for treatment because it significantly reduced the gene expression levels of MDR <em>P. aeruginosa </em>in broiler farms. |
| first_indexed | 2024-04-11T10:15:34Z |
| format | Article |
| id | doaj.art-e09db4faccd3455b9a70634fa263a46a |
| institution | Directory Open Access Journal |
| issn | 1607-3894 2071-1255 |
| language | Arabic |
| last_indexed | 2024-04-11T10:15:34Z |
| publishDate | 2022-10-01 |
| publisher | University of Mosul, College of Veterinary Medicine |
| record_format | Article |
| series | Iraqi Journal of Veterinary Sciences |
| spelling | doaj.art-e09db4faccd3455b9a70634fa263a46a2022-12-22T04:29:58ZaraUniversity of Mosul, College of Veterinary MedicineIraqi Journal of Veterinary Sciences1607-38942071-12552022-10-0136487788810.33899/ijvs.2022.132430.2094175416Effect of garlic oil nanoemulsion against multidrug resistant Pseudomonas aeruginosa isolated from broilerAmal S. El-Oksh0Dalia M. Elmasry1Ghada A. Ibrahim2Biotechnology Department, RLQP, AHRI, Sharkia Branch, ARC, EgyptNanomaterials Research and Synthesis Department, AHRI, Dokki Branch, ARC, EgyptBacteriology Department, AHRI, Ismailia branch, ARC, EgyptThis work aimed to focus on the antibacterial properties of garlic nanoemulsion on some multidrug resistance (MDR) strains of<em> Pseudomona</em>s <em>a</em><em>eruginos</em>aisolated from broiler farms and hatcheries in Sharkia and Ismailia governorates, Egypt. <em>Pseudomonas</em> spp. was isolated in 21.3% of collected samples. It was isolated from younger broilers 1-10 days with an incidence rate of 22% (11/50), older broilers 16% (8/50), dead embryo in shell 31.4% (11/35), and from hatcheries was 13.3% (2/15). There was a variable range of antibiotic resistance ranging from 66.7-100% against the isolated strains of <em>P. aeruginosa</em>. Tetracycline and sulfamethoxazole-trimethoprim achieved the highest resistance rates, while penicillin and gentamycin were of a lower rate. However, norfloxacin, ciprofloxacin, and colistin were the most sensitive antibiotics against examined MDR <em>P. aeruginosa.</em>16SrDNA gene was found in ten <em>P. aeruginosa</em> isolates. Theseisolates were found to be virulent as <em>oprL</em> gene was detected in all isolates 100%. In addition, <em>tetA(A),bla</em>TEM<em>,arr, </em>and<em> mex</em>Rantibiotic resistance genes were shown positive 100% in all MDR <em>P. aeruginosa</em> isolates. Minimum Inhibitory Concentration (MIC) values showed that garlic nanoemulsion (GN) was effective against examined <em>P. aeruginosa</em> at different concentrations. GN had 29.61% sulfur compounds of active components with 0.52 ug/ml of IC<sub>50</sub> and 40.94 nm size with polydispersity index: 0.165 using dynamic light scattering had a 19.6± 5.11mV. In conclusion, the application of garlic nanoemulsion is an excellent alternative candidate to antibiotics for treatment because it significantly reduced the gene expression levels of MDR <em>P. aeruginosa </em>in broiler farms.https://www.vetmedmosul.com/article_175416_e3d277c35a135887c219358336468878.pdfresistancepseudomonas sppgarlic oilvirulence gene |
| spellingShingle | Amal S. El-Oksh Dalia M. Elmasry Ghada A. Ibrahim Effect of garlic oil nanoemulsion against multidrug resistant Pseudomonas aeruginosa isolated from broiler Iraqi Journal of Veterinary Sciences resistance pseudomonas spp garlic oil virulence gene |
| title | Effect of garlic oil nanoemulsion against multidrug resistant Pseudomonas aeruginosa isolated from broiler |
| title_full | Effect of garlic oil nanoemulsion against multidrug resistant Pseudomonas aeruginosa isolated from broiler |
| title_fullStr | Effect of garlic oil nanoemulsion against multidrug resistant Pseudomonas aeruginosa isolated from broiler |
| title_full_unstemmed | Effect of garlic oil nanoemulsion against multidrug resistant Pseudomonas aeruginosa isolated from broiler |
| title_short | Effect of garlic oil nanoemulsion against multidrug resistant Pseudomonas aeruginosa isolated from broiler |
| title_sort | effect of garlic oil nanoemulsion against multidrug resistant pseudomonas aeruginosa isolated from broiler |
| topic | resistance pseudomonas spp garlic oil virulence gene |
| url | https://www.vetmedmosul.com/article_175416_e3d277c35a135887c219358336468878.pdf |
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