Disinfection effect of hexadecyl pyridinium chloride on SARS-CoV-2 in vitro
The novel coronavirus (COVID-19 or 2019-nCoV) is a respiratory virus that can exist in the mouth and saliva of patients and spreads through aerosol dispersion. Therefore, stomatological hospitals and departments have become high-infection-risk environments. Accordingly, oral disinfectants that can e...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Karger Publishers
2022-09-01
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| Series: | Intervirology |
| Online Access: | https://www.karger.com/Article/FullText/526241 |
| _version_ | 1797995964400664576 |
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| author | Ke-da Chen Fei-ke Ma Qing-jing Wang Ying Wang Xin-yi Zhuang Xu-ning Zhang Hai-yan Mao Yan-jun Zhang |
| author_facet | Ke-da Chen Fei-ke Ma Qing-jing Wang Ying Wang Xin-yi Zhuang Xu-ning Zhang Hai-yan Mao Yan-jun Zhang |
| author_sort | Ke-da Chen |
| collection | DOAJ |
| description | The novel coronavirus (COVID-19 or 2019-nCoV) is a respiratory virus that can exist in the mouth and saliva of patients and spreads through aerosol dispersion. Therefore, stomatological hospitals and departments have become high-infection-risk environments. Accordingly, oral disinfectants that can effectively inactivate the virus have become a highly active area of research. Hexadecyl pyridinium chloride, povidone-iodine, and other common oral disinfectants are the natural primary choices for stomatological hospitals. Therefore, this study investigated the inhibitory effect of hexadecyl pyridinium chloride on SARS-CoV-2 in vitro. Vero cells infected with SARS-CoV-2 were used to determine the disinfection effect; the CCK-8 method was used to determine cytotoxicity, and viral load was determined by real-time PCR. The results showed that hexadecyl pyridinium chloride has no obvious cytotoxic effect on Vero cells in the concentration range 0.0125–0.05 mg/mL. The in vitro experiments showed that hexadecyl pyridinium chloride significantly inhibits the virus at concentrations of 0.1 mg/mL or above at 2 min of action. Thus, the results provide experimental support for the use of hexadecyl pyridinium chloride in stomatological hospitals. |
| first_indexed | 2024-04-11T10:08:53Z |
| format | Article |
| id | doaj.art-e09f868b63c349e596e825a73a1b3745 |
| institution | Directory Open Access Journal |
| issn | 0300-5526 1423-0100 |
| language | English |
| last_indexed | 2024-04-11T10:08:53Z |
| publishDate | 2022-09-01 |
| publisher | Karger Publishers |
| record_format | Article |
| series | Intervirology |
| spelling | doaj.art-e09f868b63c349e596e825a73a1b37452022-12-22T04:30:09ZengKarger PublishersIntervirology0300-55261423-01002022-09-0110.1159/000526241526241Disinfection effect of hexadecyl pyridinium chloride on SARS-CoV-2 in vitroKe-da ChenFei-ke MaQing-jing WangYing WangXin-yi Zhuanghttps://orcid.org/0000-0001-9960-9243Xu-ning Zhanghttps://orcid.org/0000-0003-0605-9882Hai-yan MaoYan-jun ZhangThe novel coronavirus (COVID-19 or 2019-nCoV) is a respiratory virus that can exist in the mouth and saliva of patients and spreads through aerosol dispersion. Therefore, stomatological hospitals and departments have become high-infection-risk environments. Accordingly, oral disinfectants that can effectively inactivate the virus have become a highly active area of research. Hexadecyl pyridinium chloride, povidone-iodine, and other common oral disinfectants are the natural primary choices for stomatological hospitals. Therefore, this study investigated the inhibitory effect of hexadecyl pyridinium chloride on SARS-CoV-2 in vitro. Vero cells infected with SARS-CoV-2 were used to determine the disinfection effect; the CCK-8 method was used to determine cytotoxicity, and viral load was determined by real-time PCR. The results showed that hexadecyl pyridinium chloride has no obvious cytotoxic effect on Vero cells in the concentration range 0.0125–0.05 mg/mL. The in vitro experiments showed that hexadecyl pyridinium chloride significantly inhibits the virus at concentrations of 0.1 mg/mL or above at 2 min of action. Thus, the results provide experimental support for the use of hexadecyl pyridinium chloride in stomatological hospitals.https://www.karger.com/Article/FullText/526241 |
| spellingShingle | Ke-da Chen Fei-ke Ma Qing-jing Wang Ying Wang Xin-yi Zhuang Xu-ning Zhang Hai-yan Mao Yan-jun Zhang Disinfection effect of hexadecyl pyridinium chloride on SARS-CoV-2 in vitro Intervirology |
| title | Disinfection effect of hexadecyl pyridinium chloride on SARS-CoV-2 in vitro |
| title_full | Disinfection effect of hexadecyl pyridinium chloride on SARS-CoV-2 in vitro |
| title_fullStr | Disinfection effect of hexadecyl pyridinium chloride on SARS-CoV-2 in vitro |
| title_full_unstemmed | Disinfection effect of hexadecyl pyridinium chloride on SARS-CoV-2 in vitro |
| title_short | Disinfection effect of hexadecyl pyridinium chloride on SARS-CoV-2 in vitro |
| title_sort | disinfection effect of hexadecyl pyridinium chloride on sars cov 2 in vitro |
| url | https://www.karger.com/Article/FullText/526241 |
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