The first Conus genome assembly reveals a primary genetic central dogma of conopeptides in C. betulinus
Abstract Although there are various Conus species with publicly available transcriptome and proteome data, no genome assembly has been reported yet. Here, using Chinese tubular cone snail (C. betulinus) as a representative, we sequenced and assembled the first Conus genome with original identificati...
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| Format: | Article |
| Language: | English |
| Published: |
Nature Publishing Group
2021-02-01
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| Series: | Cell Discovery |
| Online Access: | https://doi.org/10.1038/s41421-021-00244-7 |
| _version_ | 1818849900095340544 |
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| author | Chao Peng Yu Huang Chao Bian Jia Li Jie Liu Kai Zhang Xinxin You Zhilong Lin Yanbin He Jieming Chen Yunyun Lv Zhiqiang Ruan Xinhui Zhang Yunhai Yi Yanping Li Xueqiang Lin Ruobo Gu Junmin Xu Jia’an Yang Chongxu Fan Ge Yao Ji-Sheng Chen Hui Jiang Bingmiao Gao Qiong Shi |
| author_facet | Chao Peng Yu Huang Chao Bian Jia Li Jie Liu Kai Zhang Xinxin You Zhilong Lin Yanbin He Jieming Chen Yunyun Lv Zhiqiang Ruan Xinhui Zhang Yunhai Yi Yanping Li Xueqiang Lin Ruobo Gu Junmin Xu Jia’an Yang Chongxu Fan Ge Yao Ji-Sheng Chen Hui Jiang Bingmiao Gao Qiong Shi |
| author_sort | Chao Peng |
| collection | DOAJ |
| description | Abstract Although there are various Conus species with publicly available transcriptome and proteome data, no genome assembly has been reported yet. Here, using Chinese tubular cone snail (C. betulinus) as a representative, we sequenced and assembled the first Conus genome with original identification of 133 genome-widely distributed conopeptide genes. After integration of our genomics, transcriptomics, and peptidomics data in the same species, we established a primary genetic central dogma of diverse conopeptides, assuming a rough number ratio of ~1:1:1:10s for the total genes: transcripts: proteins: post-translationally modified peptides. This ratio may be special for this worm-hunting Conus species, due to the high diversity of various Conus genomes and the big number ranges of conopeptide genes, transcripts, and peptides in previous reports of diverse Conus species. Only a fraction (45.9%) of the identified conotopeptide genes from our achieved genome assembly are transcribed with transcriptomic evidence, and few genes individually correspond to multiple transcripts possibly due to intraspecies or mutation-based variances. Variable peptide processing at the proteomic level, generating a big diversity of venom conopeptides with alternative cleavage sites, post-translational modifications, and N-/C-terminal truncations, may explain how the 133 genes and ~123 transcripts can generate thousands of conopeptides in the venom of individual C. betulinus. We also predicted many conopeptides with high stereostructural similarities to the putative analgesic ω-MVIIA, addiction therapy AuIB and insecticide ImI, suggesting that our current genome assembly for C. betulinus is a valuable genetic resource for high-throughput prediction and development of potential pharmaceuticals. |
| first_indexed | 2024-12-19T06:40:35Z |
| format | Article |
| id | doaj.art-e0a00a926885480ebe015ba142f251c0 |
| institution | Directory Open Access Journal |
| issn | 2056-5968 |
| language | English |
| last_indexed | 2024-12-19T06:40:35Z |
| publishDate | 2021-02-01 |
| publisher | Nature Publishing Group |
| record_format | Article |
| series | Cell Discovery |
| spelling | doaj.art-e0a00a926885480ebe015ba142f251c02022-12-21T20:32:06ZengNature Publishing GroupCell Discovery2056-59682021-02-017111410.1038/s41421-021-00244-7The first Conus genome assembly reveals a primary genetic central dogma of conopeptides in C. betulinusChao Peng0Yu Huang1Chao Bian2Jia Li3Jie Liu4Kai Zhang5Xinxin You6Zhilong Lin7Yanbin He8Jieming Chen9Yunyun Lv10Zhiqiang Ruan11Xinhui Zhang12Yunhai Yi13Yanping Li14Xueqiang Lin15Ruobo Gu16Junmin Xu17Jia’an Yang18Chongxu Fan19Ge Yao20Ji-Sheng Chen21Hui Jiang22Bingmiao Gao23Qiong Shi24Shenzhen Key Lab of Marine Genomics, Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic Animals, BGI Academy of Marine Sciences, BGI Marine, BGIShenzhen Key Lab of Marine Genomics, Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic Animals, BGI Academy of Marine Sciences, BGI Marine, BGIShenzhen Key Lab of Marine Genomics, Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic Animals, BGI Academy of Marine Sciences, BGI Marine, BGIShenzhen Key Lab of Marine Genomics, Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic Animals, BGI Academy of Marine Sciences, BGI Marine, BGIBGI-Shenzhen, BGIShenzhen Key Lab of Marine Genomics, Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic Animals, BGI Academy of Marine Sciences, BGI Marine, BGIShenzhen Key Lab of Marine Genomics, Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic Animals, BGI Academy of Marine Sciences, BGI Marine, BGIBGI-Shenzhen, BGIBGI-Shenzhen, BGIShenzhen Key Lab of Marine Genomics, Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic Animals, BGI Academy of Marine Sciences, BGI Marine, BGIShenzhen Key Lab of Marine Genomics, Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic Animals, BGI Academy of Marine Sciences, BGI Marine, BGIShenzhen Key Lab of Marine Genomics, Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic Animals, BGI Academy of Marine Sciences, BGI Marine, BGIShenzhen Key Lab of Marine Genomics, Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic Animals, BGI Academy of Marine Sciences, BGI Marine, BGIShenzhen Key Lab of Marine Genomics, Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic Animals, BGI Academy of Marine Sciences, BGI Marine, BGIShenzhen Key Lab of Marine Genomics, Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic Animals, BGI Academy of Marine Sciences, BGI Marine, BGIShenzhen Key Lab of Marine Genomics, Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic Animals, BGI Academy of Marine Sciences, BGI Marine, BGIShenzhen Key Lab of Marine Genomics, Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic Animals, BGI Academy of Marine Sciences, BGI Marine, BGIShenzhen Key Lab of Marine Genomics, Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic Animals, BGI Academy of Marine Sciences, BGI Marine, BGIMicro Pharmtech Ltd.Research Institute of Pharmaceutical ChemistryResearch Institute of Pharmaceutical ChemistryResearch Institute of Pharmaceutical ChemistryResearch Institute of Pharmaceutical ChemistryKey Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Provincial Key Laboratory of Research and Development of Herbs, School of Pharmacy, Hainan Medical UniversityShenzhen Key Lab of Marine Genomics, Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic Animals, BGI Academy of Marine Sciences, BGI Marine, BGIAbstract Although there are various Conus species with publicly available transcriptome and proteome data, no genome assembly has been reported yet. Here, using Chinese tubular cone snail (C. betulinus) as a representative, we sequenced and assembled the first Conus genome with original identification of 133 genome-widely distributed conopeptide genes. After integration of our genomics, transcriptomics, and peptidomics data in the same species, we established a primary genetic central dogma of diverse conopeptides, assuming a rough number ratio of ~1:1:1:10s for the total genes: transcripts: proteins: post-translationally modified peptides. This ratio may be special for this worm-hunting Conus species, due to the high diversity of various Conus genomes and the big number ranges of conopeptide genes, transcripts, and peptides in previous reports of diverse Conus species. Only a fraction (45.9%) of the identified conotopeptide genes from our achieved genome assembly are transcribed with transcriptomic evidence, and few genes individually correspond to multiple transcripts possibly due to intraspecies or mutation-based variances. Variable peptide processing at the proteomic level, generating a big diversity of venom conopeptides with alternative cleavage sites, post-translational modifications, and N-/C-terminal truncations, may explain how the 133 genes and ~123 transcripts can generate thousands of conopeptides in the venom of individual C. betulinus. We also predicted many conopeptides with high stereostructural similarities to the putative analgesic ω-MVIIA, addiction therapy AuIB and insecticide ImI, suggesting that our current genome assembly for C. betulinus is a valuable genetic resource for high-throughput prediction and development of potential pharmaceuticals.https://doi.org/10.1038/s41421-021-00244-7 |
| spellingShingle | Chao Peng Yu Huang Chao Bian Jia Li Jie Liu Kai Zhang Xinxin You Zhilong Lin Yanbin He Jieming Chen Yunyun Lv Zhiqiang Ruan Xinhui Zhang Yunhai Yi Yanping Li Xueqiang Lin Ruobo Gu Junmin Xu Jia’an Yang Chongxu Fan Ge Yao Ji-Sheng Chen Hui Jiang Bingmiao Gao Qiong Shi The first Conus genome assembly reveals a primary genetic central dogma of conopeptides in C. betulinus Cell Discovery |
| title | The first Conus genome assembly reveals a primary genetic central dogma of conopeptides in C. betulinus |
| title_full | The first Conus genome assembly reveals a primary genetic central dogma of conopeptides in C. betulinus |
| title_fullStr | The first Conus genome assembly reveals a primary genetic central dogma of conopeptides in C. betulinus |
| title_full_unstemmed | The first Conus genome assembly reveals a primary genetic central dogma of conopeptides in C. betulinus |
| title_short | The first Conus genome assembly reveals a primary genetic central dogma of conopeptides in C. betulinus |
| title_sort | first conus genome assembly reveals a primary genetic central dogma of conopeptides in c betulinus |
| url | https://doi.org/10.1038/s41421-021-00244-7 |
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