<i>OsbHLHq11</i>, the Basic Helix-Loop-Helix Transcription Factor, Involved in Regulation of Chlorophyll Content in Rice
Photosynthesis is an important factor in determining the yield of rice. In particular, the size and efficiency of the photosynthetic system after the heading has a great impact on the yield. Research related to high-efficiency photosynthesis is essential to meet the growing demands of crops for the...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-07-01
|
| Series: | Biology |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-7737/11/7/1000 |
| _version_ | 1827597901206913024 |
|---|---|
| author | Yoon-Hee Jang Jae-Ryoung Park Eun-Gyeong Kim Kyung-Min Kim |
| author_facet | Yoon-Hee Jang Jae-Ryoung Park Eun-Gyeong Kim Kyung-Min Kim |
| author_sort | Yoon-Hee Jang |
| collection | DOAJ |
| description | Photosynthesis is an important factor in determining the yield of rice. In particular, the size and efficiency of the photosynthetic system after the heading has a great impact on the yield. Research related to high-efficiency photosynthesis is essential to meet the growing demands of crops for the growing population. Chlorophyll is a key molecule in photosynthesis, a pigment that acts as an antenna to absorb light energy. Improvement of chlorophyll content characteristics has been emphasized in rice breeding for several decades. It is expected that an increase in chlorophyll content may increase photosynthetic efficiency, and understanding the genetic basis involved is important. In this study, we measured leaf color (CIELAB), chlorophyll content (SPAD), and chlorophyll fluorescence, and quantitative trait loci (QTL) mapping was performed using 120 Cheongcheong/Nagdong double haploid (CNDH) line after the heading date. A major QTL related to chlorophyll content was detected in the RM26981-RM287 region of chromosome 11. <i>OsbHLHq11</i> was finally selected through screening of genes related to chlorophyll content in the RM26981-RM287 region. The relative expression level of the gene of <i>OsbHLHq11</i> was highly expressed in cultivars with low chlorophyll content, and is expected to have a similar function to BHLH62 of the <i>Gramineae</i> genus. <i>OsbHLHq11</i> is expected to increase photosynthetic efficiency by being involved in the chlorophyll content, and is expected to be utilized as a new genetic resource for breeding high-yield rice. |
| first_indexed | 2024-03-09T03:41:55Z |
| format | Article |
| id | doaj.art-e0a1847089ea4b66b6cf591211dcfabe |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-09T03:41:55Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-e0a1847089ea4b66b6cf591211dcfabe2023-12-03T14:40:30ZengMDPI AGBiology2079-77372022-07-01117100010.3390/biology11071000<i>OsbHLHq11</i>, the Basic Helix-Loop-Helix Transcription Factor, Involved in Regulation of Chlorophyll Content in RiceYoon-Hee Jang0Jae-Ryoung Park1Eun-Gyeong Kim2Kyung-Min Kim3Department of Applied Biosciences, Graduate School, Kyungpook National University, Daegu 41566, KoreaCrop Breeding Division, Rural Development Administration, National Institute of Crop Science, Wanju 55365, KoreaDepartment of Applied Biosciences, Graduate School, Kyungpook National University, Daegu 41566, KoreaDepartment of Applied Biosciences, Graduate School, Kyungpook National University, Daegu 41566, KoreaPhotosynthesis is an important factor in determining the yield of rice. In particular, the size and efficiency of the photosynthetic system after the heading has a great impact on the yield. Research related to high-efficiency photosynthesis is essential to meet the growing demands of crops for the growing population. Chlorophyll is a key molecule in photosynthesis, a pigment that acts as an antenna to absorb light energy. Improvement of chlorophyll content characteristics has been emphasized in rice breeding for several decades. It is expected that an increase in chlorophyll content may increase photosynthetic efficiency, and understanding the genetic basis involved is important. In this study, we measured leaf color (CIELAB), chlorophyll content (SPAD), and chlorophyll fluorescence, and quantitative trait loci (QTL) mapping was performed using 120 Cheongcheong/Nagdong double haploid (CNDH) line after the heading date. A major QTL related to chlorophyll content was detected in the RM26981-RM287 region of chromosome 11. <i>OsbHLHq11</i> was finally selected through screening of genes related to chlorophyll content in the RM26981-RM287 region. The relative expression level of the gene of <i>OsbHLHq11</i> was highly expressed in cultivars with low chlorophyll content, and is expected to have a similar function to BHLH62 of the <i>Gramineae</i> genus. <i>OsbHLHq11</i> is expected to increase photosynthetic efficiency by being involved in the chlorophyll content, and is expected to be utilized as a new genetic resource for breeding high-yield rice.https://www.mdpi.com/2079-7737/11/7/1000CIELABphotosynthesisQTLSPADyield |
| spellingShingle | Yoon-Hee Jang Jae-Ryoung Park Eun-Gyeong Kim Kyung-Min Kim <i>OsbHLHq11</i>, the Basic Helix-Loop-Helix Transcription Factor, Involved in Regulation of Chlorophyll Content in Rice Biology CIELAB photosynthesis QTL SPAD yield |
| title | <i>OsbHLHq11</i>, the Basic Helix-Loop-Helix Transcription Factor, Involved in Regulation of Chlorophyll Content in Rice |
| title_full | <i>OsbHLHq11</i>, the Basic Helix-Loop-Helix Transcription Factor, Involved in Regulation of Chlorophyll Content in Rice |
| title_fullStr | <i>OsbHLHq11</i>, the Basic Helix-Loop-Helix Transcription Factor, Involved in Regulation of Chlorophyll Content in Rice |
| title_full_unstemmed | <i>OsbHLHq11</i>, the Basic Helix-Loop-Helix Transcription Factor, Involved in Regulation of Chlorophyll Content in Rice |
| title_short | <i>OsbHLHq11</i>, the Basic Helix-Loop-Helix Transcription Factor, Involved in Regulation of Chlorophyll Content in Rice |
| title_sort | i osbhlhq11 i the basic helix loop helix transcription factor involved in regulation of chlorophyll content in rice |
| topic | CIELAB photosynthesis QTL SPAD yield |
| url | https://www.mdpi.com/2079-7737/11/7/1000 |
| work_keys_str_mv | AT yoonheejang iosbhlhq11ithebasichelixloophelixtranscriptionfactorinvolvedinregulationofchlorophyllcontentinrice AT jaeryoungpark iosbhlhq11ithebasichelixloophelixtranscriptionfactorinvolvedinregulationofchlorophyllcontentinrice AT eungyeongkim iosbhlhq11ithebasichelixloophelixtranscriptionfactorinvolvedinregulationofchlorophyllcontentinrice AT kyungminkim iosbhlhq11ithebasichelixloophelixtranscriptionfactorinvolvedinregulationofchlorophyllcontentinrice |