Estimating the protein burden limit of yeast cells by measuring the expression limits of glycolytic proteins
The ultimate overexpression of a protein could cause growth defects, which are known as the protein burden. However, the expression limit at which the protein-burden effect is triggered is still unclear. To estimate this limit, we systematically measured the overexpression limits of glycolytic prote...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2018-08-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/34595 |
| _version_ | 1811201159704084480 |
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| author | Yuichi Eguchi Koji Makanae Tomohisa Hasunuma Yuko Ishibashi Keiji Kito Hisao Moriya |
| author_facet | Yuichi Eguchi Koji Makanae Tomohisa Hasunuma Yuko Ishibashi Keiji Kito Hisao Moriya |
| author_sort | Yuichi Eguchi |
| collection | DOAJ |
| description | The ultimate overexpression of a protein could cause growth defects, which are known as the protein burden. However, the expression limit at which the protein-burden effect is triggered is still unclear. To estimate this limit, we systematically measured the overexpression limits of glycolytic proteins in Saccharomyces cerevisiae. The limits of some glycolytic proteins were up to 15% of the total cellular protein. These limits were independent of the proteins’ catalytic activities, a finding that was supported by an in silico analysis. Some proteins had low expression limits that were explained by their localization and metabolic perturbations. The codon usage should be highly optimized to trigger the protein-burden effect, even under strong transcriptional induction. The S–S-bond-connected aggregation mediated by the cysteine residues of a protein might affect its expression limit. Theoretically, only non-harmful proteins could be expressed up to the protein-burden limit. Therefore, we established a framework to distinguish proteins that are harmful and non-harmful upon overexpression. |
| first_indexed | 2024-04-12T02:16:07Z |
| format | Article |
| id | doaj.art-e0a64d5f20db440ca7ad074a50a78832 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T02:16:07Z |
| publishDate | 2018-08-01 |
| publisher | eLife Sciences Publications Ltd |
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| series | eLife |
| spelling | doaj.art-e0a64d5f20db440ca7ad074a50a788322022-12-22T03:52:15ZengeLife Sciences Publications LtdeLife2050-084X2018-08-01710.7554/eLife.34595Estimating the protein burden limit of yeast cells by measuring the expression limits of glycolytic proteinsYuichi Eguchi0https://orcid.org/0000-0002-4809-1402Koji Makanae1Tomohisa Hasunuma2Yuko Ishibashi3Keiji Kito4Hisao Moriya5https://orcid.org/0000-0001-7638-3640Graduate School of Environmental and Life Science, Okayama University, Okayama, JapanResearch Core for Interdisciplinary Sciences, Okayama University, Okayama, JapanGraduate School of Science, Technology and Innovation, Kobe University, Kobe, JapanDepartment of Life Sciences, School of Agriculture, Meiji University, Kawasaki, JapanDepartment of Life Sciences, School of Agriculture, Meiji University, Kawasaki, JapanGraduate School of Environmental and Life Science, Okayama University, Okayama, Japan; Research Core for Interdisciplinary Sciences, Okayama University, Okayama, JapanThe ultimate overexpression of a protein could cause growth defects, which are known as the protein burden. However, the expression limit at which the protein-burden effect is triggered is still unclear. To estimate this limit, we systematically measured the overexpression limits of glycolytic proteins in Saccharomyces cerevisiae. The limits of some glycolytic proteins were up to 15% of the total cellular protein. These limits were independent of the proteins’ catalytic activities, a finding that was supported by an in silico analysis. Some proteins had low expression limits that were explained by their localization and metabolic perturbations. The codon usage should be highly optimized to trigger the protein-burden effect, even under strong transcriptional induction. The S–S-bond-connected aggregation mediated by the cysteine residues of a protein might affect its expression limit. Theoretically, only non-harmful proteins could be expressed up to the protein-burden limit. Therefore, we established a framework to distinguish proteins that are harmful and non-harmful upon overexpression.https://elifesciences.org/articles/34595protein burdenoverexpressionglycolysis |
| spellingShingle | Yuichi Eguchi Koji Makanae Tomohisa Hasunuma Yuko Ishibashi Keiji Kito Hisao Moriya Estimating the protein burden limit of yeast cells by measuring the expression limits of glycolytic proteins eLife protein burden overexpression glycolysis |
| title | Estimating the protein burden limit of yeast cells by measuring the expression limits of glycolytic proteins |
| title_full | Estimating the protein burden limit of yeast cells by measuring the expression limits of glycolytic proteins |
| title_fullStr | Estimating the protein burden limit of yeast cells by measuring the expression limits of glycolytic proteins |
| title_full_unstemmed | Estimating the protein burden limit of yeast cells by measuring the expression limits of glycolytic proteins |
| title_short | Estimating the protein burden limit of yeast cells by measuring the expression limits of glycolytic proteins |
| title_sort | estimating the protein burden limit of yeast cells by measuring the expression limits of glycolytic proteins |
| topic | protein burden overexpression glycolysis |
| url | https://elifesciences.org/articles/34595 |
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