Screening and Expression Analysis of <i>POD</i> Gene in POD-H<sub>2</sub>O<sub>2</sub> Pathway on Bud Dormancy of Pear (<i>Pyrus pyrifolia</i>)
Bud endodormancy represents a pivotal and intricate biological process influenced by both genetic and epigenetic factors, the exact mechanism of which remains elusive. Hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) functions as a signalling molecule in the regulation of dorma...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-02-01
|
| Series: | Forests |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4907/15/3/434 |
| _version_ | 1797241068107857920 |
|---|---|
| author | Xuwen Xiang Keke Song Yinyin Li Chenyu Zhang Ruiqi Zhou Yu Feng Jingnan You Jingdong Wu Yanhui Zhang Cuicui Jiang Yongyu Li |
| author_facet | Xuwen Xiang Keke Song Yinyin Li Chenyu Zhang Ruiqi Zhou Yu Feng Jingnan You Jingdong Wu Yanhui Zhang Cuicui Jiang Yongyu Li |
| author_sort | Xuwen Xiang |
| collection | DOAJ |
| description | Bud endodormancy represents a pivotal and intricate biological process influenced by both genetic and epigenetic factors, the exact mechanism of which remains elusive. Hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) functions as a signalling molecule in the regulation of dormancy, with peroxidase (POD) playing a crucial role in governing H<sub>2</sub>O<sub>2</sub> levels. Our prior transcriptomic and metabolomic investigations into diverse pear dormancy phases posited that <i>POD</i> predominantly oversees pear bud dormancy. In this study, we utilised qRT-PCR to screen the most significantly expressed gene, Pyrus pyrifolia <i>POD4-like</i> (<i>PpPOD4-like</i>), from seven <i>POD</i> genes. Subsequently, H<sub>2</sub>O<sub>2</sub> test kits, overexpression methods, and subcellular localisation techniques were employed to assess changes in H<sub>2</sub>O<sub>2</sub> content, POD activity, <i>PpPOD4-like</i> expression, and its cellular positioning during pear bud dormancy. Subcellular localisation experiments revealed that PpPOD4-like is situated on the cell membranes. Notably, H<sub>2</sub>O<sub>2</sub> content exhibited a rapid increase during endodormancy and decreased swiftly after ecodormancy. The fluctuation pattern of POD activity aligned with that of H<sub>2</sub>O<sub>2</sub> content. Additionally, <i>PpPOD4-like</i> expression was markedly upregulated, displaying an overall upward trajectory. Our findings indicate that <i>PpPOD4-like</i> modulates H<sub>2</sub>O<sub>2</sub> levels by regulating POD activity, thereby actively participating in the intricate regulation of pear dormancy processes. |
| first_indexed | 2024-04-24T18:17:27Z |
| format | Article |
| id | doaj.art-e0abcfa6cc1c43d8bbcc3e07e1ee24c1 |
| institution | Directory Open Access Journal |
| issn | 1999-4907 |
| language | English |
| last_indexed | 2024-04-24T18:17:27Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Forests |
| spelling | doaj.art-e0abcfa6cc1c43d8bbcc3e07e1ee24c12024-03-27T13:41:34ZengMDPI AGForests1999-49072024-02-0115343410.3390/f15030434Screening and Expression Analysis of <i>POD</i> Gene in POD-H<sub>2</sub>O<sub>2</sub> Pathway on Bud Dormancy of Pear (<i>Pyrus pyrifolia</i>)Xuwen Xiang0Keke Song1Yinyin Li2Chenyu Zhang3Ruiqi Zhou4Yu Feng5Jingnan You6Jingdong Wu7Yanhui Zhang8Cuicui Jiang9Yongyu Li10Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaKey Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaKey Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaKey Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaKey Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaKey Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaKey Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaEconomic Crop Station, Agricultural and Rural Bureau of Jianning County, Sanming 354500, ChinaEconomic Crop Station, Agricultural and Rural Bureau of Yongtai County, Fuzhou 350700, ChinaFruit Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350002, ChinaKey Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaBud endodormancy represents a pivotal and intricate biological process influenced by both genetic and epigenetic factors, the exact mechanism of which remains elusive. Hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) functions as a signalling molecule in the regulation of dormancy, with peroxidase (POD) playing a crucial role in governing H<sub>2</sub>O<sub>2</sub> levels. Our prior transcriptomic and metabolomic investigations into diverse pear dormancy phases posited that <i>POD</i> predominantly oversees pear bud dormancy. In this study, we utilised qRT-PCR to screen the most significantly expressed gene, Pyrus pyrifolia <i>POD4-like</i> (<i>PpPOD4-like</i>), from seven <i>POD</i> genes. Subsequently, H<sub>2</sub>O<sub>2</sub> test kits, overexpression methods, and subcellular localisation techniques were employed to assess changes in H<sub>2</sub>O<sub>2</sub> content, POD activity, <i>PpPOD4-like</i> expression, and its cellular positioning during pear bud dormancy. Subcellular localisation experiments revealed that PpPOD4-like is situated on the cell membranes. Notably, H<sub>2</sub>O<sub>2</sub> content exhibited a rapid increase during endodormancy and decreased swiftly after ecodormancy. The fluctuation pattern of POD activity aligned with that of H<sub>2</sub>O<sub>2</sub> content. Additionally, <i>PpPOD4-like</i> expression was markedly upregulated, displaying an overall upward trajectory. Our findings indicate that <i>PpPOD4-like</i> modulates H<sub>2</sub>O<sub>2</sub> levels by regulating POD activity, thereby actively participating in the intricate regulation of pear dormancy processes.https://www.mdpi.com/1999-4907/15/3/434endodormancyoverexpressionH<sub>2</sub>O<sub>2</sub><i>POD</i> genes |
| spellingShingle | Xuwen Xiang Keke Song Yinyin Li Chenyu Zhang Ruiqi Zhou Yu Feng Jingnan You Jingdong Wu Yanhui Zhang Cuicui Jiang Yongyu Li Screening and Expression Analysis of <i>POD</i> Gene in POD-H<sub>2</sub>O<sub>2</sub> Pathway on Bud Dormancy of Pear (<i>Pyrus pyrifolia</i>) Forests endodormancy overexpression H<sub>2</sub>O<sub>2</sub> <i>POD</i> genes |
| title | Screening and Expression Analysis of <i>POD</i> Gene in POD-H<sub>2</sub>O<sub>2</sub> Pathway on Bud Dormancy of Pear (<i>Pyrus pyrifolia</i>) |
| title_full | Screening and Expression Analysis of <i>POD</i> Gene in POD-H<sub>2</sub>O<sub>2</sub> Pathway on Bud Dormancy of Pear (<i>Pyrus pyrifolia</i>) |
| title_fullStr | Screening and Expression Analysis of <i>POD</i> Gene in POD-H<sub>2</sub>O<sub>2</sub> Pathway on Bud Dormancy of Pear (<i>Pyrus pyrifolia</i>) |
| title_full_unstemmed | Screening and Expression Analysis of <i>POD</i> Gene in POD-H<sub>2</sub>O<sub>2</sub> Pathway on Bud Dormancy of Pear (<i>Pyrus pyrifolia</i>) |
| title_short | Screening and Expression Analysis of <i>POD</i> Gene in POD-H<sub>2</sub>O<sub>2</sub> Pathway on Bud Dormancy of Pear (<i>Pyrus pyrifolia</i>) |
| title_sort | screening and expression analysis of i pod i gene in pod h sub 2 sub o sub 2 sub pathway on bud dormancy of pear i pyrus pyrifolia i |
| topic | endodormancy overexpression H<sub>2</sub>O<sub>2</sub> <i>POD</i> genes |
| url | https://www.mdpi.com/1999-4907/15/3/434 |
| work_keys_str_mv | AT xuwenxiang screeningandexpressionanalysisofipodigeneinpodhsub2subosub2subpathwayonbuddormancyofpearipyruspyrifoliai AT kekesong screeningandexpressionanalysisofipodigeneinpodhsub2subosub2subpathwayonbuddormancyofpearipyruspyrifoliai AT yinyinli screeningandexpressionanalysisofipodigeneinpodhsub2subosub2subpathwayonbuddormancyofpearipyruspyrifoliai AT chenyuzhang screeningandexpressionanalysisofipodigeneinpodhsub2subosub2subpathwayonbuddormancyofpearipyruspyrifoliai AT ruiqizhou screeningandexpressionanalysisofipodigeneinpodhsub2subosub2subpathwayonbuddormancyofpearipyruspyrifoliai AT yufeng screeningandexpressionanalysisofipodigeneinpodhsub2subosub2subpathwayonbuddormancyofpearipyruspyrifoliai AT jingnanyou screeningandexpressionanalysisofipodigeneinpodhsub2subosub2subpathwayonbuddormancyofpearipyruspyrifoliai AT jingdongwu screeningandexpressionanalysisofipodigeneinpodhsub2subosub2subpathwayonbuddormancyofpearipyruspyrifoliai AT yanhuizhang screeningandexpressionanalysisofipodigeneinpodhsub2subosub2subpathwayonbuddormancyofpearipyruspyrifoliai AT cuicuijiang screeningandexpressionanalysisofipodigeneinpodhsub2subosub2subpathwayonbuddormancyofpearipyruspyrifoliai AT yongyuli screeningandexpressionanalysisofipodigeneinpodhsub2subosub2subpathwayonbuddormancyofpearipyruspyrifoliai |