Sensing ultrasound localization microscopy for the visualization of glomeruli in living rats and humansResearch in context
Summary: Background: Estimation of glomerular function is necessary to diagnose kidney diseases. However, the study of glomeruli in the clinic is currently done indirectly through urine and blood tests. A recent imaging technique called Ultrasound Localization Microscopy (ULM) has appeared. It is b...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-05-01
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| Series: | EBioMedicine |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352396423001433 |
| _version_ | 1797842473283747840 |
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| author | Louise Denis Sylvain Bodard Vincent Hingot Arthur Chavignon Jacques Battaglia Gilles Renault Franck Lager Abderrahmane Aissani Olivier Hélénon Jean-Michel Correas Olivier Couture |
| author_facet | Louise Denis Sylvain Bodard Vincent Hingot Arthur Chavignon Jacques Battaglia Gilles Renault Franck Lager Abderrahmane Aissani Olivier Hélénon Jean-Michel Correas Olivier Couture |
| author_sort | Louise Denis |
| collection | DOAJ |
| description | Summary: Background: Estimation of glomerular function is necessary to diagnose kidney diseases. However, the study of glomeruli in the clinic is currently done indirectly through urine and blood tests. A recent imaging technique called Ultrasound Localization Microscopy (ULM) has appeared. It is based on the ability to record continuous movements of individual microbubbles in the bloodstream. Although ULM improved the resolution of vascular imaging up to tenfold, the imaging of the smallest vessels had yet to be reported. Methods: We acquired ultrasound sequences from living humans and rats and then applied filters to divide the data set into slow-moving and fast-moving microbubbles. We performed a double tracking to highlight and characterize populations of microbubbles with singular behaviors. We decided to call this technique “sensing ULM” (sULM). We used post-mortem micro-CT for side-by-side confirmation in rats. Findings: In this study, we report the observation of microbubbles flowing in the glomeruli in living humans and rats. We present a set of analysis tools to extract quantitative information from individual microbubbles, such as remanence time or normalized distance. Interpretation: As glomeruli play a key role in kidney function, it would be possible that their observation yields a deeper understanding of the kidney. It could also be a tool to diagnose kidney diseases in patients. More generally, it will bring imaging capabilities closer to the functional units of organs, which is a key to understand most diseases, such as cancer, diabetes, or kidney failures. Funding: This study was funded by the European Research Council under the European Union Horizon H2020 program (ERC Consolidator grant agreement No 772786-ResolveStroke). |
| first_indexed | 2024-04-09T16:48:28Z |
| format | Article |
| id | doaj.art-e0ad1a8f57404aa08e530115bce150f2 |
| institution | Directory Open Access Journal |
| issn | 2352-3964 |
| language | English |
| last_indexed | 2024-04-09T16:48:28Z |
| publishDate | 2023-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | EBioMedicine |
| spelling | doaj.art-e0ad1a8f57404aa08e530115bce150f22023-04-22T06:21:06ZengElsevierEBioMedicine2352-39642023-05-0191104578Sensing ultrasound localization microscopy for the visualization of glomeruli in living rats and humansResearch in contextLouise Denis0Sylvain Bodard1Vincent Hingot2Arthur Chavignon3Jacques Battaglia4Gilles Renault5Franck Lager6Abderrahmane Aissani7Olivier Hélénon8Jean-Michel Correas9Olivier Couture10Sorbonne Université, CNRS, INSERM Laboratoire d’Imagerie Biomédicale, F-75006, Paris, France; Corresponding author.Sorbonne Université, CNRS, INSERM Laboratoire d’Imagerie Biomédicale, F-75006, Paris, France; AP-HP, Hôpital Necker Enfants Malades, Service d’Imagerie Adulte, F-75015, Paris, France; Université de Paris Cité, F-75006, Paris, FranceSorbonne Université, CNRS, INSERM Laboratoire d’Imagerie Biomédicale, F-75006, Paris, FranceSorbonne Université, CNRS, INSERM Laboratoire d’Imagerie Biomédicale, F-75006, Paris, FranceSorbonne Université, CNRS, INSERM Laboratoire d’Imagerie Biomédicale, F-75006, Paris, FranceUniversité de Paris, Institut Cochin, INSERM, CNRS, F-75014, Paris, FranceUniversité de Paris, Institut Cochin, INSERM, CNRS, F-75014, Paris, FranceSorbonne Université, CNRS, INSERM Laboratoire d’Imagerie Biomédicale, F-75006, Paris, FranceAP-HP, Hôpital Necker Enfants Malades, Service d’Imagerie Adulte, F-75015, Paris, France; Université de Paris Cité, F-75006, Paris, FranceSorbonne Université, CNRS, INSERM Laboratoire d’Imagerie Biomédicale, F-75006, Paris, France; AP-HP, Hôpital Necker Enfants Malades, Service d’Imagerie Adulte, F-75015, Paris, France; Université de Paris Cité, F-75006, Paris, FranceSorbonne Université, CNRS, INSERM Laboratoire d’Imagerie Biomédicale, F-75006, Paris, FranceSummary: Background: Estimation of glomerular function is necessary to diagnose kidney diseases. However, the study of glomeruli in the clinic is currently done indirectly through urine and blood tests. A recent imaging technique called Ultrasound Localization Microscopy (ULM) has appeared. It is based on the ability to record continuous movements of individual microbubbles in the bloodstream. Although ULM improved the resolution of vascular imaging up to tenfold, the imaging of the smallest vessels had yet to be reported. Methods: We acquired ultrasound sequences from living humans and rats and then applied filters to divide the data set into slow-moving and fast-moving microbubbles. We performed a double tracking to highlight and characterize populations of microbubbles with singular behaviors. We decided to call this technique “sensing ULM” (sULM). We used post-mortem micro-CT for side-by-side confirmation in rats. Findings: In this study, we report the observation of microbubbles flowing in the glomeruli in living humans and rats. We present a set of analysis tools to extract quantitative information from individual microbubbles, such as remanence time or normalized distance. Interpretation: As glomeruli play a key role in kidney function, it would be possible that their observation yields a deeper understanding of the kidney. It could also be a tool to diagnose kidney diseases in patients. More generally, it will bring imaging capabilities closer to the functional units of organs, which is a key to understand most diseases, such as cancer, diabetes, or kidney failures. Funding: This study was funded by the European Research Council under the European Union Horizon H2020 program (ERC Consolidator grant agreement No 772786-ResolveStroke).http://www.sciencedirect.com/science/article/pii/S2352396423001433Sensing Ultrasound Localization MicroscopySuper resolutionGlomeruliHuman kidneyRat kidneyIn vivo |
| spellingShingle | Louise Denis Sylvain Bodard Vincent Hingot Arthur Chavignon Jacques Battaglia Gilles Renault Franck Lager Abderrahmane Aissani Olivier Hélénon Jean-Michel Correas Olivier Couture Sensing ultrasound localization microscopy for the visualization of glomeruli in living rats and humansResearch in context EBioMedicine Sensing Ultrasound Localization Microscopy Super resolution Glomeruli Human kidney Rat kidney In vivo |
| title | Sensing ultrasound localization microscopy for the visualization of glomeruli in living rats and humansResearch in context |
| title_full | Sensing ultrasound localization microscopy for the visualization of glomeruli in living rats and humansResearch in context |
| title_fullStr | Sensing ultrasound localization microscopy for the visualization of glomeruli in living rats and humansResearch in context |
| title_full_unstemmed | Sensing ultrasound localization microscopy for the visualization of glomeruli in living rats and humansResearch in context |
| title_short | Sensing ultrasound localization microscopy for the visualization of glomeruli in living rats and humansResearch in context |
| title_sort | sensing ultrasound localization microscopy for the visualization of glomeruli in living rats and humansresearch in context |
| topic | Sensing Ultrasound Localization Microscopy Super resolution Glomeruli Human kidney Rat kidney In vivo |
| url | http://www.sciencedirect.com/science/article/pii/S2352396423001433 |
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