Molecular epidemiology of cattle tuberculosis in Mexico through whole-genome sequencing and spoligotyping.
Mycobacterium bovis infection in cattle persists in Mexico, posing a threat to human health. Control of bovine tuberculosis, through the National Program Against Bovine Tuberculosis, has led to the decrease of disease prevalence in most of the country, except for high dairy production regions. Genot...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2018-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC6107157?pdf=render |
| _version_ | 1819072131227451392 |
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| author | Claudia Angélica Perea Razo Elba Rodríguez Hernández Sergio Iván Román Ponce Feliciano Milián Suazo Suelee Robbe-Austerman Tod Stuber Germinal Jorge Cantó Alarcón |
| author_facet | Claudia Angélica Perea Razo Elba Rodríguez Hernández Sergio Iván Román Ponce Feliciano Milián Suazo Suelee Robbe-Austerman Tod Stuber Germinal Jorge Cantó Alarcón |
| author_sort | Claudia Angélica Perea Razo |
| collection | DOAJ |
| description | Mycobacterium bovis infection in cattle persists in Mexico, posing a threat to human health. Control of bovine tuberculosis, through the National Program Against Bovine Tuberculosis, has led to the decrease of disease prevalence in most of the country, except for high dairy production regions. Genotyping of M. bovis has been performed mainly by spoligotyping and variable number tandem repeats (VNTR), but higher resolution power can be useful for a finer definition of the spread of the disease. Whole genome sequencing and spoligotyping was performed for a set of 322 M. bovis isolates from different sources in Mexico: Baja California, Coahuila, Estado de Mexico, Guanajuato, Hidalgo, Jalisco, Queretaro and Veracruz, from dairy and beef cattle, as well as humans. Twelve main genetic clades were obtained through WGS and genetic diversity analysis. A clear differentiation of the Baja California isolates was seen as they clustered together exclusively. However, isolates from the central states showed no specific clustering whatsoever. Although WGS proves to have higher resolving power than spoligotyping, and since there was concordance between WGS and spoligotyping results, we consider that the latter is still an efficient and practical method for monitoring bovine tuberculosis in developing countries, where resources for higher technology are scarce. |
| first_indexed | 2024-12-21T17:32:51Z |
| format | Article |
| id | doaj.art-e0adaab564f9469b8477681b294fbb34 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T17:32:51Z |
| publishDate | 2018-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-e0adaab564f9469b8477681b294fbb342022-12-21T18:55:53ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-01138e020198110.1371/journal.pone.0201981Molecular epidemiology of cattle tuberculosis in Mexico through whole-genome sequencing and spoligotyping.Claudia Angélica Perea RazoElba Rodríguez HernándezSergio Iván Román PonceFeliciano Milián SuazoSuelee Robbe-AustermanTod StuberGerminal Jorge Cantó AlarcónMycobacterium bovis infection in cattle persists in Mexico, posing a threat to human health. Control of bovine tuberculosis, through the National Program Against Bovine Tuberculosis, has led to the decrease of disease prevalence in most of the country, except for high dairy production regions. Genotyping of M. bovis has been performed mainly by spoligotyping and variable number tandem repeats (VNTR), but higher resolution power can be useful for a finer definition of the spread of the disease. Whole genome sequencing and spoligotyping was performed for a set of 322 M. bovis isolates from different sources in Mexico: Baja California, Coahuila, Estado de Mexico, Guanajuato, Hidalgo, Jalisco, Queretaro and Veracruz, from dairy and beef cattle, as well as humans. Twelve main genetic clades were obtained through WGS and genetic diversity analysis. A clear differentiation of the Baja California isolates was seen as they clustered together exclusively. However, isolates from the central states showed no specific clustering whatsoever. Although WGS proves to have higher resolving power than spoligotyping, and since there was concordance between WGS and spoligotyping results, we consider that the latter is still an efficient and practical method for monitoring bovine tuberculosis in developing countries, where resources for higher technology are scarce.http://europepmc.org/articles/PMC6107157?pdf=render |
| spellingShingle | Claudia Angélica Perea Razo Elba Rodríguez Hernández Sergio Iván Román Ponce Feliciano Milián Suazo Suelee Robbe-Austerman Tod Stuber Germinal Jorge Cantó Alarcón Molecular epidemiology of cattle tuberculosis in Mexico through whole-genome sequencing and spoligotyping. PLoS ONE |
| title | Molecular epidemiology of cattle tuberculosis in Mexico through whole-genome sequencing and spoligotyping. |
| title_full | Molecular epidemiology of cattle tuberculosis in Mexico through whole-genome sequencing and spoligotyping. |
| title_fullStr | Molecular epidemiology of cattle tuberculosis in Mexico through whole-genome sequencing and spoligotyping. |
| title_full_unstemmed | Molecular epidemiology of cattle tuberculosis in Mexico through whole-genome sequencing and spoligotyping. |
| title_short | Molecular epidemiology of cattle tuberculosis in Mexico through whole-genome sequencing and spoligotyping. |
| title_sort | molecular epidemiology of cattle tuberculosis in mexico through whole genome sequencing and spoligotyping |
| url | http://europepmc.org/articles/PMC6107157?pdf=render |
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