Total Flavonoids in Caragana (TFC) Promotes Angiogenesis and Enhances Cerebral Perfusion in a Rat Model of Ischemic Stroke
Previous studies have demonstrated that total flavonoid extracts from Caragana sinica (TFC) exert multiple therapeutic effects, promote blood flow, and exhibit anti-inflammatory and antioxidant properties. The present study aimed to investigate whether TFC promotes angiogenesis and exerts neuroprote...
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Frontiers Media S.A.
2018-09-01
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| Series: | Frontiers in Neuroscience |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fnins.2018.00635/full |
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| author | Qiansong He Shirong Li Lailai Li Feiran Hu Ning Weng Xiaodi Fan Shixiang Kuang |
| author_facet | Qiansong He Shirong Li Lailai Li Feiran Hu Ning Weng Xiaodi Fan Shixiang Kuang |
| author_sort | Qiansong He |
| collection | DOAJ |
| description | Previous studies have demonstrated that total flavonoid extracts from Caragana sinica (TFC) exert multiple therapeutic effects, promote blood flow, and exhibit anti-inflammatory and antioxidant properties. The present study aimed to investigate whether TFC promotes angiogenesis and exerts neuroprotective effects in a rat model of transient middle cerebral artery occlusion (tMCAO). Male Wistar rats were subjected to tMCAO for 1.5 h, followed by 24 h of reperfusion. TFC (15, 30, 60 mg/kg) was administered for 14 days. Evaluations of neurological function were performed following reperfusion, and infarct volumes were assessed in brain slices stained with 2,3,5-triphenyltetrazolium chloride (TTC). Our results indicated that TFC significantly attenuated cerebral infarct volume and neurological deficits following tMCAO. Laser Doppler, micro-PET/CT, and MRI analyses further demonstrated that TFC reduced infarct volume and enhanced cerebral blood flow in a dose-dependent manner, with the most significant effects occurring at a concentration of 60 mg/kg. Significant up-regulation of CD31, VEGF, Ang-1, HIF-1α, delta-like 4 (Dll4), and Notch1 expression was also observed in the experimental groups, relative to that in the vehicle group. In summary, the results of the present study indicate that TFC (15, 30, 60 mg/kg) attenuates neurological deficits, reduces infarct volume, and promotes angiogenesis following MCAO in a concentration-dependent manner, likely via increases in the expression of CD31, VEGF, Ang-1, HIF-1α, Dll4, and Notch1. Further studies are required to determine the clinical usefulness and potential mechanisms of TFC in patients with cerebral focal ischemic stroke. |
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| language | English |
| last_indexed | 2024-12-11T18:42:54Z |
| publishDate | 2018-09-01 |
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| series | Frontiers in Neuroscience |
| spelling | doaj.art-e0afbba8ab9045f48aa0e954b811413e2022-12-22T00:54:34ZengFrontiers Media S.A.Frontiers in Neuroscience1662-453X2018-09-011210.3389/fnins.2018.00635396376Total Flavonoids in Caragana (TFC) Promotes Angiogenesis and Enhances Cerebral Perfusion in a Rat Model of Ischemic StrokeQiansong He0Shirong Li1Lailai Li2Feiran Hu3Ning Weng4Xiaodi Fan5Shixiang Kuang6Guiyang College of Traditional Chinese Medicine, Guiyang, ChinaDepartment of Neurology, Guizhou Provincial People’s Hospital, Guiyang, ChinaGuiyang College of Traditional Chinese Medicine, Guiyang, ChinaGuiyang College of Traditional Chinese Medicine, Guiyang, ChinaGuiyang College of Traditional Chinese Medicine, Guiyang, ChinaDepartment of Experimental Research Center, Xiyuan Hospital of China Academy of Chinese Medical Sciences, Beijing, ChinaGuiyang College of Traditional Chinese Medicine, Guiyang, ChinaPrevious studies have demonstrated that total flavonoid extracts from Caragana sinica (TFC) exert multiple therapeutic effects, promote blood flow, and exhibit anti-inflammatory and antioxidant properties. The present study aimed to investigate whether TFC promotes angiogenesis and exerts neuroprotective effects in a rat model of transient middle cerebral artery occlusion (tMCAO). Male Wistar rats were subjected to tMCAO for 1.5 h, followed by 24 h of reperfusion. TFC (15, 30, 60 mg/kg) was administered for 14 days. Evaluations of neurological function were performed following reperfusion, and infarct volumes were assessed in brain slices stained with 2,3,5-triphenyltetrazolium chloride (TTC). Our results indicated that TFC significantly attenuated cerebral infarct volume and neurological deficits following tMCAO. Laser Doppler, micro-PET/CT, and MRI analyses further demonstrated that TFC reduced infarct volume and enhanced cerebral blood flow in a dose-dependent manner, with the most significant effects occurring at a concentration of 60 mg/kg. Significant up-regulation of CD31, VEGF, Ang-1, HIF-1α, delta-like 4 (Dll4), and Notch1 expression was also observed in the experimental groups, relative to that in the vehicle group. In summary, the results of the present study indicate that TFC (15, 30, 60 mg/kg) attenuates neurological deficits, reduces infarct volume, and promotes angiogenesis following MCAO in a concentration-dependent manner, likely via increases in the expression of CD31, VEGF, Ang-1, HIF-1α, Dll4, and Notch1. Further studies are required to determine the clinical usefulness and potential mechanisms of TFC in patients with cerebral focal ischemic stroke.https://www.frontiersin.org/article/10.3389/fnins.2018.00635/fullTFCtMCAOangiogenesisneurological functioninfarct volumes |
| spellingShingle | Qiansong He Shirong Li Lailai Li Feiran Hu Ning Weng Xiaodi Fan Shixiang Kuang Total Flavonoids in Caragana (TFC) Promotes Angiogenesis and Enhances Cerebral Perfusion in a Rat Model of Ischemic Stroke Frontiers in Neuroscience TFC tMCAO angiogenesis neurological function infarct volumes |
| title | Total Flavonoids in Caragana (TFC) Promotes Angiogenesis and Enhances Cerebral Perfusion in a Rat Model of Ischemic Stroke |
| title_full | Total Flavonoids in Caragana (TFC) Promotes Angiogenesis and Enhances Cerebral Perfusion in a Rat Model of Ischemic Stroke |
| title_fullStr | Total Flavonoids in Caragana (TFC) Promotes Angiogenesis and Enhances Cerebral Perfusion in a Rat Model of Ischemic Stroke |
| title_full_unstemmed | Total Flavonoids in Caragana (TFC) Promotes Angiogenesis and Enhances Cerebral Perfusion in a Rat Model of Ischemic Stroke |
| title_short | Total Flavonoids in Caragana (TFC) Promotes Angiogenesis and Enhances Cerebral Perfusion in a Rat Model of Ischemic Stroke |
| title_sort | total flavonoids in caragana tfc promotes angiogenesis and enhances cerebral perfusion in a rat model of ischemic stroke |
| topic | TFC tMCAO angiogenesis neurological function infarct volumes |
| url | https://www.frontiersin.org/article/10.3389/fnins.2018.00635/full |
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