Antigenicity and adhesiveness of a Plasmodium vivax VIR-E protein from Brazilian isolates
BACKGROUND Plasmodium vivax, the major cause of malaria in Latin America, has a large subtelomeric multigene family called vir. In the P. vivax genome, about 20% of its sequences are vir genes. Vir antigens are grouped in subfamilies according to their sequence similarities and have been shown to h...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2022-02-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762021000101110&tlng=en |
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| author | Ana Paula Schappo Najara C Bittencourt Leticia P Bertolla Sofia Forcellini Ana Beatriz Iung Enembreck da Silva Hellen Geremias dos Santos João Henrique Gervásio Marcus VG Lacerda Stefanie CP Lopes Fabio TM Costa Letusa Albrecht |
| author_facet | Ana Paula Schappo Najara C Bittencourt Leticia P Bertolla Sofia Forcellini Ana Beatriz Iung Enembreck da Silva Hellen Geremias dos Santos João Henrique Gervásio Marcus VG Lacerda Stefanie CP Lopes Fabio TM Costa Letusa Albrecht |
| author_sort | Ana Paula Schappo |
| collection | DOAJ |
| description | BACKGROUND Plasmodium vivax, the major cause of malaria in Latin America, has a large subtelomeric multigene family called vir. In the P. vivax genome, about 20% of its sequences are vir genes. Vir antigens are grouped in subfamilies according to their sequence similarities and have been shown to have distinct roles and subcellular locations. However, little is known about vir subfamilies, especially when comes to their functions. OBJECTIVE To evaluate the diversity, antigenicity, and adhesiveness of Plasmodium vivax VIR-E. METHODS Vir-E genes were amplified from six P. vivax isolates from Manaus, North of Brazil. The presence of naturally acquired antibodies to recombinant PvBrVIR-E and PvAMA-1 was evaluated by ELISA. Binding capacity of recombinant PvBrVIR-E was assessed by adhesion assay to CHO-ICAM1 cells. FINDINGS Despite vir-E sequence diversity, among those identified sequences, a representative one was chosen to be expressed as recombinant protein. The presence of IgM or IgG antibodies to PvBrVIR-E was detected in 23.75% of the study population while the presence of IgG antibodies to PvAMA-1 antigen was 66.25% in the same population. PvBrVIR-E was adhesive to CHO-ICAM1. MAIN CONCLUSIONS PvBrVIR-E was antigenic and adhesive to CHO-ICAM1. |
| first_indexed | 2024-03-12T08:11:14Z |
| format | Article |
| id | doaj.art-e0afc9fcd0b94265b3fe0ca3c8a4028c |
| institution | Directory Open Access Journal |
| issn | 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T08:11:14Z |
| publishDate | 2022-02-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-e0afc9fcd0b94265b3fe0ca3c8a4028c2023-09-02T19:07:04ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-80602022-02-0111610.1590/0074-02760210227Antigenicity and adhesiveness of a Plasmodium vivax VIR-E protein from Brazilian isolatesAna Paula SchappoNajara C BittencourtLeticia P BertollaSofia ForcelliniAna Beatriz Iung Enembreck da SilvaHellen Geremias dos SantosJoão Henrique GervásioMarcus VG LacerdaStefanie CP LopesFabio TM CostaLetusa Albrechthttps://orcid.org/0000-0001-6406-2057 BACKGROUND Plasmodium vivax, the major cause of malaria in Latin America, has a large subtelomeric multigene family called vir. In the P. vivax genome, about 20% of its sequences are vir genes. Vir antigens are grouped in subfamilies according to their sequence similarities and have been shown to have distinct roles and subcellular locations. However, little is known about vir subfamilies, especially when comes to their functions. OBJECTIVE To evaluate the diversity, antigenicity, and adhesiveness of Plasmodium vivax VIR-E. METHODS Vir-E genes were amplified from six P. vivax isolates from Manaus, North of Brazil. The presence of naturally acquired antibodies to recombinant PvBrVIR-E and PvAMA-1 was evaluated by ELISA. Binding capacity of recombinant PvBrVIR-E was assessed by adhesion assay to CHO-ICAM1 cells. FINDINGS Despite vir-E sequence diversity, among those identified sequences, a representative one was chosen to be expressed as recombinant protein. The presence of IgM or IgG antibodies to PvBrVIR-E was detected in 23.75% of the study population while the presence of IgG antibodies to PvAMA-1 antigen was 66.25% in the same population. PvBrVIR-E was adhesive to CHO-ICAM1. MAIN CONCLUSIONS PvBrVIR-E was antigenic and adhesive to CHO-ICAM1.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762021000101110&tlng=enPlasmodium vivaxmultigene familyantigenicityadhesiveness |
| spellingShingle | Ana Paula Schappo Najara C Bittencourt Leticia P Bertolla Sofia Forcellini Ana Beatriz Iung Enembreck da Silva Hellen Geremias dos Santos João Henrique Gervásio Marcus VG Lacerda Stefanie CP Lopes Fabio TM Costa Letusa Albrecht Antigenicity and adhesiveness of a Plasmodium vivax VIR-E protein from Brazilian isolates Memorias do Instituto Oswaldo Cruz Plasmodium vivax multigene family antigenicity adhesiveness |
| title | Antigenicity and adhesiveness of a Plasmodium vivax VIR-E protein from Brazilian isolates |
| title_full | Antigenicity and adhesiveness of a Plasmodium vivax VIR-E protein from Brazilian isolates |
| title_fullStr | Antigenicity and adhesiveness of a Plasmodium vivax VIR-E protein from Brazilian isolates |
| title_full_unstemmed | Antigenicity and adhesiveness of a Plasmodium vivax VIR-E protein from Brazilian isolates |
| title_short | Antigenicity and adhesiveness of a Plasmodium vivax VIR-E protein from Brazilian isolates |
| title_sort | antigenicity and adhesiveness of a plasmodium vivax vir e protein from brazilian isolates |
| topic | Plasmodium vivax multigene family antigenicity adhesiveness |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762021000101110&tlng=en |
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