Gastric juice microbiota in pediatric chronic gastritis that clinically tested positive and negative for Helicobacter pylori
PurposeHelicobacter pylori (HP) infection is an identified risk factor for pediatric chronic gastritis (PCG), but its impact on gastric juice microbiota (GJM) remains to be further elucidated in PCG. This study aimed to analyze and compare the microbial communities and microbial interactive networks...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-04-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1112709/full |
| _version_ | 1797839425444511744 |
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| author | Ying Chen Shou-Yue Xia Fu-Xia Ru Jun-Jie Feng Ji Tao Zhi-Yuan Wei Xiu Li Chengjia Qian Qiong Lin Jian-Huan Chen |
| author_facet | Ying Chen Shou-Yue Xia Fu-Xia Ru Jun-Jie Feng Ji Tao Zhi-Yuan Wei Xiu Li Chengjia Qian Qiong Lin Jian-Huan Chen |
| author_sort | Ying Chen |
| collection | DOAJ |
| description | PurposeHelicobacter pylori (HP) infection is an identified risk factor for pediatric chronic gastritis (PCG), but its impact on gastric juice microbiota (GJM) remains to be further elucidated in PCG. This study aimed to analyze and compare the microbial communities and microbial interactive networks of GJM in PCG that clinically tested positive and negative for HP (HP+ and HP−, respectively).MethodsA total of 45 PCG patients aged from 6 to 16 years were recruited, including 20 HP+ and 25 HP− patients tested by culture and rapid urease test. Gastric juice samples were collected from these PCG patients and subjected to high-throughput amplicon sequencing and subsequent analysis of 16S rRNA genes.ResultsWhile no significant change in alpha diversity, significant differences in beta diversity were observed between HP+ and HP− PCG. At the genus level, Streptococcus, Helicobacter, and Granulicatella were significantly enriched in HP+ PCG, whereas Campylobacter and Absconditabacteriales (SR1) were significantly enriched in HP− PCG. Network analysis showed that Streptococcus was the only genus positively correlated with Helicobacter (r = 0.497) in the GJM network of overall PCG. Moreover, compared to HP− PCG, HP+ PCG showed a reduction in microbial network connectivity in GJM. Netshift analysis identified driver microbes including Streptococcus and other four genera, which substantially contributed to the GJM network transition from HP− PCG to HP+ PCG. Furthermore, Predicted GJM function analysis indicated up-regulated pathways related to the metabolism of nucleotides, carbohydrates, and L-Lysine, the urea cycle, as well as endotoxin peptidoglycan biosynthesis and maturation in HP+ PCG.ConclusionGJM in HP+ PCG exhibited dramatically altered beta diversity, taxonomic structure, and function, with reduced microbial network connectivity, which could be involved in the disease etiology. |
| first_indexed | 2024-04-09T15:57:47Z |
| format | Article |
| id | doaj.art-e0b20521778a4962aace672f72146e64 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-09T15:57:47Z |
| publishDate | 2023-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-e0b20521778a4962aace672f72146e642023-04-25T15:06:23ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-04-011410.3389/fmicb.2023.11127091112709Gastric juice microbiota in pediatric chronic gastritis that clinically tested positive and negative for Helicobacter pyloriYing Chen0Shou-Yue Xia1Fu-Xia Ru2Jun-Jie Feng3Ji Tao4Zhi-Yuan Wei5Xiu Li6Chengjia Qian7Qiong Lin8Jian-Huan Chen9Department of Gastroenterology, Affiliated Children’s Hospital of Jiangnan University, Wuxi, ChinaLaboratory of Genomic and Precision Medicine, Wuxi School of Medicine, Jiangnan University, Wuxi, Jiangsu, ChinaLaboratory of Genomic and Precision Medicine, Wuxi School of Medicine, Jiangnan University, Wuxi, Jiangsu, ChinaLaboratory of Genomic and Precision Medicine, Wuxi School of Medicine, Jiangnan University, Wuxi, Jiangsu, ChinaLaboratory of Genomic and Precision Medicine, Wuxi School of Medicine, Jiangnan University, Wuxi, Jiangsu, ChinaLaboratory of Genomic and Precision Medicine, Wuxi School of Medicine, Jiangnan University, Wuxi, Jiangsu, ChinaLaboratory Animal Center, Jiangnan University, Wuxi, Jiangsu, ChinaDepartment of General Surgery, Affiliated Hospital of Jiangnan University, Wuxi, ChinaDepartment of Gastroenterology, Affiliated Children’s Hospital of Jiangnan University, Wuxi, ChinaLaboratory of Genomic and Precision Medicine, Wuxi School of Medicine, Jiangnan University, Wuxi, Jiangsu, ChinaPurposeHelicobacter pylori (HP) infection is an identified risk factor for pediatric chronic gastritis (PCG), but its impact on gastric juice microbiota (GJM) remains to be further elucidated in PCG. This study aimed to analyze and compare the microbial communities and microbial interactive networks of GJM in PCG that clinically tested positive and negative for HP (HP+ and HP−, respectively).MethodsA total of 45 PCG patients aged from 6 to 16 years were recruited, including 20 HP+ and 25 HP− patients tested by culture and rapid urease test. Gastric juice samples were collected from these PCG patients and subjected to high-throughput amplicon sequencing and subsequent analysis of 16S rRNA genes.ResultsWhile no significant change in alpha diversity, significant differences in beta diversity were observed between HP+ and HP− PCG. At the genus level, Streptococcus, Helicobacter, and Granulicatella were significantly enriched in HP+ PCG, whereas Campylobacter and Absconditabacteriales (SR1) were significantly enriched in HP− PCG. Network analysis showed that Streptococcus was the only genus positively correlated with Helicobacter (r = 0.497) in the GJM network of overall PCG. Moreover, compared to HP− PCG, HP+ PCG showed a reduction in microbial network connectivity in GJM. Netshift analysis identified driver microbes including Streptococcus and other four genera, which substantially contributed to the GJM network transition from HP− PCG to HP+ PCG. Furthermore, Predicted GJM function analysis indicated up-regulated pathways related to the metabolism of nucleotides, carbohydrates, and L-Lysine, the urea cycle, as well as endotoxin peptidoglycan biosynthesis and maturation in HP+ PCG.ConclusionGJM in HP+ PCG exhibited dramatically altered beta diversity, taxonomic structure, and function, with reduced microbial network connectivity, which could be involved in the disease etiology.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1112709/fullgastric microbiotaHelicobacter pyloripediatric chronic gastritisnetwork of microbial interactionStreptococcus |
| spellingShingle | Ying Chen Shou-Yue Xia Fu-Xia Ru Jun-Jie Feng Ji Tao Zhi-Yuan Wei Xiu Li Chengjia Qian Qiong Lin Jian-Huan Chen Gastric juice microbiota in pediatric chronic gastritis that clinically tested positive and negative for Helicobacter pylori Frontiers in Microbiology gastric microbiota Helicobacter pylori pediatric chronic gastritis network of microbial interaction Streptococcus |
| title | Gastric juice microbiota in pediatric chronic gastritis that clinically tested positive and negative for Helicobacter pylori |
| title_full | Gastric juice microbiota in pediatric chronic gastritis that clinically tested positive and negative for Helicobacter pylori |
| title_fullStr | Gastric juice microbiota in pediatric chronic gastritis that clinically tested positive and negative for Helicobacter pylori |
| title_full_unstemmed | Gastric juice microbiota in pediatric chronic gastritis that clinically tested positive and negative for Helicobacter pylori |
| title_short | Gastric juice microbiota in pediatric chronic gastritis that clinically tested positive and negative for Helicobacter pylori |
| title_sort | gastric juice microbiota in pediatric chronic gastritis that clinically tested positive and negative for helicobacter pylori |
| topic | gastric microbiota Helicobacter pylori pediatric chronic gastritis network of microbial interaction Streptococcus |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1112709/full |
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