Nudel and FAK as antagonizing strength modulators of nascent adhesions through paxillin.
Adhesion and detachment are coordinated critical steps during cell migration. Conceptually, efficient migration requires both effective stabilization of membrane protrusions at the leading edge via nascent adhesions and their successful persistence during retraction of the trailing side via disrupti...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2009-05-01
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| Series: | PLoS Biology |
| Online Access: | http://europepmc.org/articles/PMC2684528?pdf=render |
| _version_ | 1818624028056748032 |
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| author | Yongli Shan Lihou Yu Yan Li Youdong Pan Qiangge Zhang Fubin Wang Jianfeng Chen Xueliang Zhu |
| author_facet | Yongli Shan Lihou Yu Yan Li Youdong Pan Qiangge Zhang Fubin Wang Jianfeng Chen Xueliang Zhu |
| author_sort | Yongli Shan |
| collection | DOAJ |
| description | Adhesion and detachment are coordinated critical steps during cell migration. Conceptually, efficient migration requires both effective stabilization of membrane protrusions at the leading edge via nascent adhesions and their successful persistence during retraction of the trailing side via disruption of focal adhesions. As nascent adhesions are much smaller in size than focal adhesions, they are expected to exhibit a stronger adhesivity in order to achieve the coordination between cell front and back. Here, we show that Nudel knockdown by interference RNA (RNAi) resulted in cell edge shrinkage due to poor adhesions of membrane protrusions. Nudel bound to paxillin, a scaffold protein of focal contacts, and colocalized with it in areas of active membrane protrusions, presumably at nascent adhesions. The Nudel-paxillin interaction was disrupted by focal adhesion kinase (FAK) in a paxillin-binding-dependent manner. Forced localization of Nudel in all focal contacts by fusing it to paxillin markedly strengthened their adhesivity, whereas overexpression of structurally activated FAK or any paxillin-binding FAK mutant lacking the N-terminal autoinhibitory domain caused cell edge shrinkage. These results suggest a novel mechanism for selective reinforcement of nascent adhesions via interplays of Nudel and FAK with paxillin to facilitate cell migration. |
| first_indexed | 2024-12-16T18:50:26Z |
| format | Article |
| id | doaj.art-e0b32ea7395d49038a458ff06d0477ff |
| institution | Directory Open Access Journal |
| issn | 1544-9173 1545-7885 |
| language | English |
| last_indexed | 2024-12-16T18:50:26Z |
| publishDate | 2009-05-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Biology |
| spelling | doaj.art-e0b32ea7395d49038a458ff06d0477ff2022-12-21T22:20:43ZengPublic Library of Science (PLoS)PLoS Biology1544-91731545-78852009-05-0175e100011610.1371/journal.pbio.1000116Nudel and FAK as antagonizing strength modulators of nascent adhesions through paxillin.Yongli ShanLihou YuYan LiYoudong PanQiangge ZhangFubin WangJianfeng ChenXueliang ZhuAdhesion and detachment are coordinated critical steps during cell migration. Conceptually, efficient migration requires both effective stabilization of membrane protrusions at the leading edge via nascent adhesions and their successful persistence during retraction of the trailing side via disruption of focal adhesions. As nascent adhesions are much smaller in size than focal adhesions, they are expected to exhibit a stronger adhesivity in order to achieve the coordination between cell front and back. Here, we show that Nudel knockdown by interference RNA (RNAi) resulted in cell edge shrinkage due to poor adhesions of membrane protrusions. Nudel bound to paxillin, a scaffold protein of focal contacts, and colocalized with it in areas of active membrane protrusions, presumably at nascent adhesions. The Nudel-paxillin interaction was disrupted by focal adhesion kinase (FAK) in a paxillin-binding-dependent manner. Forced localization of Nudel in all focal contacts by fusing it to paxillin markedly strengthened their adhesivity, whereas overexpression of structurally activated FAK or any paxillin-binding FAK mutant lacking the N-terminal autoinhibitory domain caused cell edge shrinkage. These results suggest a novel mechanism for selective reinforcement of nascent adhesions via interplays of Nudel and FAK with paxillin to facilitate cell migration.http://europepmc.org/articles/PMC2684528?pdf=render |
| spellingShingle | Yongli Shan Lihou Yu Yan Li Youdong Pan Qiangge Zhang Fubin Wang Jianfeng Chen Xueliang Zhu Nudel and FAK as antagonizing strength modulators of nascent adhesions through paxillin. PLoS Biology |
| title | Nudel and FAK as antagonizing strength modulators of nascent adhesions through paxillin. |
| title_full | Nudel and FAK as antagonizing strength modulators of nascent adhesions through paxillin. |
| title_fullStr | Nudel and FAK as antagonizing strength modulators of nascent adhesions through paxillin. |
| title_full_unstemmed | Nudel and FAK as antagonizing strength modulators of nascent adhesions through paxillin. |
| title_short | Nudel and FAK as antagonizing strength modulators of nascent adhesions through paxillin. |
| title_sort | nudel and fak as antagonizing strength modulators of nascent adhesions through paxillin |
| url | http://europepmc.org/articles/PMC2684528?pdf=render |
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