Editome landscape of CCM-derived endothelial cells
By regulating several phases of gene expression, RNA editing modifications contribute to maintaining physiological RNA expression levels. RNA editing dysregulation can affect RNA molecule half-life, coding/noncoding RNA interaction, alternative splicing, and circular RNA biogenesis. Impaired RNA edi...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2022-12-01
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| Series: | RNA Biology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/15476286.2022.2091306 |
| _version_ | 1797404002677161984 |
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| author | Concetta Scimone Simona Alibrandi Luigi Donato Concetta Alafaci Antonino Germanò Sergio L. Vinci Rosalia D’Angelo Antonina Sidoti |
| author_facet | Concetta Scimone Simona Alibrandi Luigi Donato Concetta Alafaci Antonino Germanò Sergio L. Vinci Rosalia D’Angelo Antonina Sidoti |
| author_sort | Concetta Scimone |
| collection | DOAJ |
| description | By regulating several phases of gene expression, RNA editing modifications contribute to maintaining physiological RNA expression levels. RNA editing dysregulation can affect RNA molecule half-life, coding/noncoding RNA interaction, alternative splicing, and circular RNA biogenesis. Impaired RNA editing has been observed in several pathological conditions, including cancer and Alzheimer’s disease. No data has been published yet on the editome profile of endothelial cells (ECs) isolated from human cerebral cavernous malformation (CCM) lesions. Here, we describe a landscape of editome modifications in sporadic CCM-derived ECs (CCM-ECs) by comparing editing events with those observed in human brain microvascular endothelial cells (HBMECs). With a whole transcriptome-based variant calling pipeline, we identified differential edited genes in CCM-ECs that were enriched in pathways related to angiogenesis, apoptosis and cell survival, inflammation and, in particular, to thrombin signalling mediated by protease-activated receptors and non-canonical Wnt signalling. These pathways, not yet associated to CCM development, could be a novel field for further investigations on CCM molecular mechanisms. Moreover, enrichment analysis of differentially edited miRNAs suggested additional small noncoding transcripts to consider for development of targeted therapies. |
| first_indexed | 2024-03-09T02:46:49Z |
| format | Article |
| id | doaj.art-e0b49f6d91e04dc791ebe92e6963e43e |
| institution | Directory Open Access Journal |
| issn | 1547-6286 1555-8584 |
| language | English |
| last_indexed | 2024-03-09T02:46:49Z |
| publishDate | 2022-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | RNA Biology |
| spelling | doaj.art-e0b49f6d91e04dc791ebe92e6963e43e2023-12-05T16:09:51ZengTaylor & Francis GroupRNA Biology1547-62861555-85842022-12-0119185286510.1080/15476286.2022.20913062091306Editome landscape of CCM-derived endothelial cellsConcetta Scimone0Simona Alibrandi1Luigi Donato2Concetta Alafaci3Antonino Germanò4Sergio L. Vinci5Rosalia D’Angelo6Antonina Sidoti7University of MessinaUniversity of MessinaUniversity of MessinaUniversity of MessinaUniversity of MessinaUniversity of MessinaUniversity of MessinaUniversity of MessinaBy regulating several phases of gene expression, RNA editing modifications contribute to maintaining physiological RNA expression levels. RNA editing dysregulation can affect RNA molecule half-life, coding/noncoding RNA interaction, alternative splicing, and circular RNA biogenesis. Impaired RNA editing has been observed in several pathological conditions, including cancer and Alzheimer’s disease. No data has been published yet on the editome profile of endothelial cells (ECs) isolated from human cerebral cavernous malformation (CCM) lesions. Here, we describe a landscape of editome modifications in sporadic CCM-derived ECs (CCM-ECs) by comparing editing events with those observed in human brain microvascular endothelial cells (HBMECs). With a whole transcriptome-based variant calling pipeline, we identified differential edited genes in CCM-ECs that were enriched in pathways related to angiogenesis, apoptosis and cell survival, inflammation and, in particular, to thrombin signalling mediated by protease-activated receptors and non-canonical Wnt signalling. These pathways, not yet associated to CCM development, could be a novel field for further investigations on CCM molecular mechanisms. Moreover, enrichment analysis of differentially edited miRNAs suggested additional small noncoding transcripts to consider for development of targeted therapies.http://dx.doi.org/10.1080/15476286.2022.2091306cerebral cavernous malformationeditome profileprotease-activated receptor signallingrna sequencing analysisdifferentially edited genes |
| spellingShingle | Concetta Scimone Simona Alibrandi Luigi Donato Concetta Alafaci Antonino Germanò Sergio L. Vinci Rosalia D’Angelo Antonina Sidoti Editome landscape of CCM-derived endothelial cells RNA Biology cerebral cavernous malformation editome profile protease-activated receptor signalling rna sequencing analysis differentially edited genes |
| title | Editome landscape of CCM-derived endothelial cells |
| title_full | Editome landscape of CCM-derived endothelial cells |
| title_fullStr | Editome landscape of CCM-derived endothelial cells |
| title_full_unstemmed | Editome landscape of CCM-derived endothelial cells |
| title_short | Editome landscape of CCM-derived endothelial cells |
| title_sort | editome landscape of ccm derived endothelial cells |
| topic | cerebral cavernous malformation editome profile protease-activated receptor signalling rna sequencing analysis differentially edited genes |
| url | http://dx.doi.org/10.1080/15476286.2022.2091306 |
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