Specification of Differentiated Adult Progenitors via Inhibition of Endocycle Entry in the Drosophila Trachea
A population of Drosophila adult tracheal progenitor cells arises from differentiated cells of the larval main trachea that retain the ability to reenter the cell cycle and give rise to the multiple adult tracheal cell types. These progenitors are unique to the second tracheal metamere as homologous...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2014-11-01
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| Series: | Cell Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124714008262 |
| _version_ | 1819237747729104896 |
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| author | Nareg J.-V. Djabrayan Josefa Cruz Cristina de Miguel Xavier Franch-Marro Jordi Casanova |
| author_facet | Nareg J.-V. Djabrayan Josefa Cruz Cristina de Miguel Xavier Franch-Marro Jordi Casanova |
| author_sort | Nareg J.-V. Djabrayan |
| collection | DOAJ |
| description | A population of Drosophila adult tracheal progenitor cells arises from differentiated cells of the larval main trachea that retain the ability to reenter the cell cycle and give rise to the multiple adult tracheal cell types. These progenitors are unique to the second tracheal metamere as homologous cells from other segments, express fizzy-related (fzr), the Drosophila homolog of CDH1 protein of the APC complex, and enter endocycle and do not contribute to adult trachea. Here, we examine the mechanisms for their quiescence and show that they reenter the cell cycle by expression of string/cdc25 through ecdysone. Furthermore, we show that preventing endocycle entry is both necessary and sufficient for these tracheal cells to exhibit markers of adult progenitors, thus modifying their genetic program. Finally, we show that Hox-mediated regulation of fzr expression is responsible for progenitor identity and thus specifies a group of differentiated cells with facultative stem cell features. |
| first_indexed | 2024-12-23T13:25:15Z |
| format | Article |
| id | doaj.art-e0b59864b9014168ab59514ddc4b7bb2 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-12-23T13:25:15Z |
| publishDate | 2014-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-e0b59864b9014168ab59514ddc4b7bb22022-12-21T17:45:19ZengElsevierCell Reports2211-12472014-11-019385986510.1016/j.celrep.2014.09.043Specification of Differentiated Adult Progenitors via Inhibition of Endocycle Entry in the Drosophila TracheaNareg J.-V. Djabrayan0Josefa Cruz1Cristina de Miguel2Xavier Franch-Marro3Jordi Casanova4Institut de Biología Molecular de Barcelona (CSIC), Carrer de Baldiri Reixac 10, 08028 Barcelona, Catalonia, SpainInstitut de Biologia Evolutiva (CSIC-UPF), Platja de la Barceloneta 37, 08003 Barcelona, Catalonia, SpainInstitut de Biología Molecular de Barcelona (CSIC), Carrer de Baldiri Reixac 10, 08028 Barcelona, Catalonia, SpainInstitut de Biologia Evolutiva (CSIC-UPF), Platja de la Barceloneta 37, 08003 Barcelona, Catalonia, SpainInstitut de Biología Molecular de Barcelona (CSIC), Carrer de Baldiri Reixac 10, 08028 Barcelona, Catalonia, SpainA population of Drosophila adult tracheal progenitor cells arises from differentiated cells of the larval main trachea that retain the ability to reenter the cell cycle and give rise to the multiple adult tracheal cell types. These progenitors are unique to the second tracheal metamere as homologous cells from other segments, express fizzy-related (fzr), the Drosophila homolog of CDH1 protein of the APC complex, and enter endocycle and do not contribute to adult trachea. Here, we examine the mechanisms for their quiescence and show that they reenter the cell cycle by expression of string/cdc25 through ecdysone. Furthermore, we show that preventing endocycle entry is both necessary and sufficient for these tracheal cells to exhibit markers of adult progenitors, thus modifying their genetic program. Finally, we show that Hox-mediated regulation of fzr expression is responsible for progenitor identity and thus specifies a group of differentiated cells with facultative stem cell features.http://www.sciencedirect.com/science/article/pii/S2211124714008262 |
| spellingShingle | Nareg J.-V. Djabrayan Josefa Cruz Cristina de Miguel Xavier Franch-Marro Jordi Casanova Specification of Differentiated Adult Progenitors via Inhibition of Endocycle Entry in the Drosophila Trachea Cell Reports |
| title | Specification of Differentiated Adult Progenitors via Inhibition of Endocycle Entry in the Drosophila Trachea |
| title_full | Specification of Differentiated Adult Progenitors via Inhibition of Endocycle Entry in the Drosophila Trachea |
| title_fullStr | Specification of Differentiated Adult Progenitors via Inhibition of Endocycle Entry in the Drosophila Trachea |
| title_full_unstemmed | Specification of Differentiated Adult Progenitors via Inhibition of Endocycle Entry in the Drosophila Trachea |
| title_short | Specification of Differentiated Adult Progenitors via Inhibition of Endocycle Entry in the Drosophila Trachea |
| title_sort | specification of differentiated adult progenitors via inhibition of endocycle entry in the drosophila trachea |
| url | http://www.sciencedirect.com/science/article/pii/S2211124714008262 |
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