Recent insights into catechins-rich Assam tea extract for photoaging and senescent ageing
Abstract Tea (Camellia spp.) is an important medicinal herb. C. sinensis var. sinensis is the most studied tea variety due to its more preferred flavor than C. sinensis var. assamica (Assam tea), the less economic importance with more bitter variety. A bitter taste highlights its potential as a cand...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-01-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-52781-2 |
| _version_ | 1797276642666610688 |
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| author | Mayuree Kanlayavattanakul Mattaka Khongkow Wannita Klinngam Puxvadee Chaikul Nattaya Lourith Piyaporn Chueamchaitrakun |
| author_facet | Mayuree Kanlayavattanakul Mattaka Khongkow Wannita Klinngam Puxvadee Chaikul Nattaya Lourith Piyaporn Chueamchaitrakun |
| author_sort | Mayuree Kanlayavattanakul |
| collection | DOAJ |
| description | Abstract Tea (Camellia spp.) is an important medicinal herb. C. sinensis var. sinensis is the most studied tea variety due to its more preferred flavor than C. sinensis var. assamica (Assam tea), the less economic importance with more bitter variety. A bitter taste highlights its potential as a candidate source for tea catechins, the health beneficial actives applicable for ageing treatment. Nonetheless, indicative data for tea on UV-induced and senescent ageing remain unclarified. Assam tea extract (ATE) was prepared and standardized in terms of TPC, TFC and TTC. EGCG was HPLC quantified as the prime ATE catechin. In vitro antioxidant activity of ATE was exhibited with ABTS, DPPH and FRAP assays. ATE’s cellular antioxidant activity was indicated in HDFs at a stronger degree than ascorbic acid. The photoaging protection of ATE was evidenced in a coculture of HaCaT cells and HDFs. ATE markedly suppressed UV-induced IL-6, IL-8, MMP-1 and MMP-9 expressions. The proficiency of ATE targeting on senescent ageing was demonstrated in an ex vivo human skin model, where IL-6 and MMP-1 expressions were suppressed, whilst hyaluronic acid and collagen syntheses were promoted. ATE was chemically stabled as indicated by the catechin contents and color parameters following 6 months storage under conditions recommended for topical product. ATE enriched in catechins warrants its applicability as a new generation of photoaging protectant agent promising for the prevention and treatment for senescent ageing. The findings indicate the proficiency of ATE for innovative anti-ageing agent. |
| first_indexed | 2024-03-07T15:31:09Z |
| format | Article |
| id | doaj.art-e0c07468b24942b0b2ba73f45121d131 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-07T15:31:09Z |
| publishDate | 2024-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-e0c07468b24942b0b2ba73f45121d1312024-03-05T16:25:34ZengNature PortfolioScientific Reports2045-23222024-01-0114111310.1038/s41598-024-52781-2Recent insights into catechins-rich Assam tea extract for photoaging and senescent ageingMayuree Kanlayavattanakul0Mattaka Khongkow1Wannita Klinngam2Puxvadee Chaikul3Nattaya Lourith4Piyaporn Chueamchaitrakun5School of Cosmetic Science, Mae Fah Luang UniversityNational Nanotechnology Center (NANOTEC), National Science and Technology Development AgencyNational Nanotechnology Center (NANOTEC), National Science and Technology Development AgencySchool of Cosmetic Science, Mae Fah Luang UniversitySchool of Cosmetic Science, Mae Fah Luang UniversitySchool of Agro-Industry, Mae Fah Luang UniversityAbstract Tea (Camellia spp.) is an important medicinal herb. C. sinensis var. sinensis is the most studied tea variety due to its more preferred flavor than C. sinensis var. assamica (Assam tea), the less economic importance with more bitter variety. A bitter taste highlights its potential as a candidate source for tea catechins, the health beneficial actives applicable for ageing treatment. Nonetheless, indicative data for tea on UV-induced and senescent ageing remain unclarified. Assam tea extract (ATE) was prepared and standardized in terms of TPC, TFC and TTC. EGCG was HPLC quantified as the prime ATE catechin. In vitro antioxidant activity of ATE was exhibited with ABTS, DPPH and FRAP assays. ATE’s cellular antioxidant activity was indicated in HDFs at a stronger degree than ascorbic acid. The photoaging protection of ATE was evidenced in a coculture of HaCaT cells and HDFs. ATE markedly suppressed UV-induced IL-6, IL-8, MMP-1 and MMP-9 expressions. The proficiency of ATE targeting on senescent ageing was demonstrated in an ex vivo human skin model, where IL-6 and MMP-1 expressions were suppressed, whilst hyaluronic acid and collagen syntheses were promoted. ATE was chemically stabled as indicated by the catechin contents and color parameters following 6 months storage under conditions recommended for topical product. ATE enriched in catechins warrants its applicability as a new generation of photoaging protectant agent promising for the prevention and treatment for senescent ageing. The findings indicate the proficiency of ATE for innovative anti-ageing agent.https://doi.org/10.1038/s41598-024-52781-2 |
| spellingShingle | Mayuree Kanlayavattanakul Mattaka Khongkow Wannita Klinngam Puxvadee Chaikul Nattaya Lourith Piyaporn Chueamchaitrakun Recent insights into catechins-rich Assam tea extract for photoaging and senescent ageing Scientific Reports |
| title | Recent insights into catechins-rich Assam tea extract for photoaging and senescent ageing |
| title_full | Recent insights into catechins-rich Assam tea extract for photoaging and senescent ageing |
| title_fullStr | Recent insights into catechins-rich Assam tea extract for photoaging and senescent ageing |
| title_full_unstemmed | Recent insights into catechins-rich Assam tea extract for photoaging and senescent ageing |
| title_short | Recent insights into catechins-rich Assam tea extract for photoaging and senescent ageing |
| title_sort | recent insights into catechins rich assam tea extract for photoaging and senescent ageing |
| url | https://doi.org/10.1038/s41598-024-52781-2 |
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