Molecular characterization of ginger genotypes using RAPD and SSR markers

Genetic diversity among ginger genotypes collected from different parts of the country was studied using molecular markers (30 RAPD and 55 SSR). Compared to RAPD primers SSR primers were efficient in distinguishing the genotypes. A total of 86 and 23 polymorphic bands were observed with RAPD and SS...

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Main Authors: Akshitha HJ, Prasath D, Umesha K, Mohammed Faisal P, Venkataravanappa V
Format: Article
Language:English
Published: Society for Promotion of Horticulture - Indian Institute of Horticultural Research 2022-09-01
Series:Journal of Horticultural Sciences
Subjects:
Online Access:https://jhs.iihr.res.in/index.php/jhs/article/view/1052
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author Akshitha HJ
Prasath D
Umesha K
Mohammed Faisal P
Venkataravanappa V
author_facet Akshitha HJ
Prasath D
Umesha K
Mohammed Faisal P
Venkataravanappa V
author_sort Akshitha HJ
collection DOAJ
description Genetic diversity among ginger genotypes collected from different parts of the country was studied using molecular markers (30 RAPD and 55 SSR). Compared to RAPD primers SSR primers were efficient in distinguishing the genotypes. A total of 86 and 23 polymorphic bands were observed with RAPD and SSR primers, respectively. Percentage polymorphism observed between RAPD and SSR primers was 97.40 % and 56.54 %. Grouping of genotypes by using combined data of RAPD and SSR primers indicated that irrespective of their place of collection or geographical origin, 30 genotypes were clustered into different groups which showed that, each individual genotype is having wider variability or it might be due to the genetic similarity existing among them.
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spelling doaj.art-e0c56618ddd14a319e26dda94273b27f2023-08-24T05:07:59ZengSociety for Promotion of Horticulture - Indian Institute of Horticultural ResearchJournal of Horticultural Sciences0973-354X2582-48992022-09-0117110.24154/jhs.v17i1.10521040Molecular characterization of ginger genotypes using RAPD and SSR markersAkshitha HJ0Prasath D1Umesha K2Mohammed Faisal P3Venkataravanappa V4College of Horticulture, UHS Campus, Bengaluru, Karnataka, IndiaICAR-Indian Institute of Spices Research, Kozhikode, Kerala, IndiaCollege of Horticulture, UHS Campus, Bengaluru, Karnataka, IndiaICAR-Indian Institute of Spices Research Regional Station, Appangala, Karnataka, IndiaICAR-IIHR Central Horticultural Experiment Station, Chettalli, Karnataka, India Genetic diversity among ginger genotypes collected from different parts of the country was studied using molecular markers (30 RAPD and 55 SSR). Compared to RAPD primers SSR primers were efficient in distinguishing the genotypes. A total of 86 and 23 polymorphic bands were observed with RAPD and SSR primers, respectively. Percentage polymorphism observed between RAPD and SSR primers was 97.40 % and 56.54 %. Grouping of genotypes by using combined data of RAPD and SSR primers indicated that irrespective of their place of collection or geographical origin, 30 genotypes were clustered into different groups which showed that, each individual genotype is having wider variability or it might be due to the genetic similarity existing among them. https://jhs.iihr.res.in/index.php/jhs/article/view/1052gingermolecular markersmonomorphicpolymorphic
spellingShingle Akshitha HJ
Prasath D
Umesha K
Mohammed Faisal P
Venkataravanappa V
Molecular characterization of ginger genotypes using RAPD and SSR markers
Journal of Horticultural Sciences
ginger
molecular markers
monomorphic
polymorphic
title Molecular characterization of ginger genotypes using RAPD and SSR markers
title_full Molecular characterization of ginger genotypes using RAPD and SSR markers
title_fullStr Molecular characterization of ginger genotypes using RAPD and SSR markers
title_full_unstemmed Molecular characterization of ginger genotypes using RAPD and SSR markers
title_short Molecular characterization of ginger genotypes using RAPD and SSR markers
title_sort molecular characterization of ginger genotypes using rapd and ssr markers
topic ginger
molecular markers
monomorphic
polymorphic
url https://jhs.iihr.res.in/index.php/jhs/article/view/1052
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