Transfluthrin and Metofluthrin as Effective Repellents against Pyrethroid-Susceptible and Pyrethroid-Resistant <i>Aedes aegypti</i> (L.) (Diptera: Culicidae)
<i>Aedes aegypti</i> is a major vector of dengue fever in tropical regions. Spatial repellents (SRs) have shown promise in delaying pesticide resistance. Methods for discriminating concentrations (DCs) are well established using various bioassay tools, while data for high-throughput scre...
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MDPI AG
2023-09-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/14/9/767 |
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| author | Dae-Yun Kim Jeffrey Hii Theeraphap Chareonviriyaphap |
| author_facet | Dae-Yun Kim Jeffrey Hii Theeraphap Chareonviriyaphap |
| author_sort | Dae-Yun Kim |
| collection | DOAJ |
| description | <i>Aedes aegypti</i> is a major vector of dengue fever in tropical regions. Spatial repellents (SRs) have shown promise in delaying pesticide resistance. Methods for discriminating concentrations (DCs) are well established using various bioassay tools, while data for high-throughput screening system (HITSS) toxicity bioassay (TOX) are absent. In this study, we compared and optimized lethal (LCs) and sub-lethal concentrations (SLCs) of transfluthrin (TFT) and metofluthrin (MFT) on pyrethroid-susceptible (USDA) and pyrethroid-resistant (Pu-Teuy) <i>Ae. aegypti</i> (L.) strains, using the HITSS-TOX. Mean mortality (MT) was 100% at LC<sub>99</sub> and DC, compared to LC<sub>50</sub> (45.0 ± 3.7%) and LC<sub>75</sub> (65.8 ± 7.0%) for the USDA strain. However, the resistant strain (Pu-Teuy) showed reduced susceptibility against TFT and a significantly lower MT at LC<sub>50</sub> (12.5 ± 4.4%; t = 5.665, df = 10, <i>p</i> < 0.001), LC<sub>75</sub> (9.2 ± 3.5%; t = 4.844, df = 10, <i>p</i> = 0.001), LC<sub>99</sub> (55.0 ± 9.9%; t = 4.538, df = 5, <i>p</i> = 0.006), and DC (75.0 ± 5.2%; U = 3.0, <i>p</i> = 0.007). The DC of TFT (0.15222%) was 4.7-fold higher than for MFT (0.03242%) in USDA strain. The baseline DCs established are useful to better understand susceptibility and the efficacy of various repellents against field populations of <i>Ae. aegypti</i>. |
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| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-10T22:38:39Z |
| publishDate | 2023-09-01 |
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| series | Insects |
| spelling | doaj.art-e0ca7c53377546c6864849544deabb6f2023-11-19T11:15:37ZengMDPI AGInsects2075-44502023-09-0114976710.3390/insects14090767Transfluthrin and Metofluthrin as Effective Repellents against Pyrethroid-Susceptible and Pyrethroid-Resistant <i>Aedes aegypti</i> (L.) (Diptera: Culicidae)Dae-Yun Kim0Jeffrey Hii1Theeraphap Chareonviriyaphap2Department of Entomology, Faculty of Agriculture, Kasetsart University, Bangkok 10900, ThailandCollege of Public Health, Medical & Veterinary Sciences, James Cook University, Brisbane, QLD 4000, AustraliaDepartment of Entomology, Faculty of Agriculture, Kasetsart University, Bangkok 10900, Thailand<i>Aedes aegypti</i> is a major vector of dengue fever in tropical regions. Spatial repellents (SRs) have shown promise in delaying pesticide resistance. Methods for discriminating concentrations (DCs) are well established using various bioassay tools, while data for high-throughput screening system (HITSS) toxicity bioassay (TOX) are absent. In this study, we compared and optimized lethal (LCs) and sub-lethal concentrations (SLCs) of transfluthrin (TFT) and metofluthrin (MFT) on pyrethroid-susceptible (USDA) and pyrethroid-resistant (Pu-Teuy) <i>Ae. aegypti</i> (L.) strains, using the HITSS-TOX. Mean mortality (MT) was 100% at LC<sub>99</sub> and DC, compared to LC<sub>50</sub> (45.0 ± 3.7%) and LC<sub>75</sub> (65.8 ± 7.0%) for the USDA strain. However, the resistant strain (Pu-Teuy) showed reduced susceptibility against TFT and a significantly lower MT at LC<sub>50</sub> (12.5 ± 4.4%; t = 5.665, df = 10, <i>p</i> < 0.001), LC<sub>75</sub> (9.2 ± 3.5%; t = 4.844, df = 10, <i>p</i> = 0.001), LC<sub>99</sub> (55.0 ± 9.9%; t = 4.538, df = 5, <i>p</i> = 0.006), and DC (75.0 ± 5.2%; U = 3.0, <i>p</i> = 0.007). The DC of TFT (0.15222%) was 4.7-fold higher than for MFT (0.03242%) in USDA strain. The baseline DCs established are useful to better understand susceptibility and the efficacy of various repellents against field populations of <i>Ae. aegypti</i>.https://www.mdpi.com/2075-4450/14/9/767<i>Aedes aegypti</i>spatial repellenthigh-throughput screening systemtoxicity bioassaytransfluthrinmetofluthrin |
| spellingShingle | Dae-Yun Kim Jeffrey Hii Theeraphap Chareonviriyaphap Transfluthrin and Metofluthrin as Effective Repellents against Pyrethroid-Susceptible and Pyrethroid-Resistant <i>Aedes aegypti</i> (L.) (Diptera: Culicidae) Insects <i>Aedes aegypti</i> spatial repellent high-throughput screening system toxicity bioassay transfluthrin metofluthrin |
| title | Transfluthrin and Metofluthrin as Effective Repellents against Pyrethroid-Susceptible and Pyrethroid-Resistant <i>Aedes aegypti</i> (L.) (Diptera: Culicidae) |
| title_full | Transfluthrin and Metofluthrin as Effective Repellents against Pyrethroid-Susceptible and Pyrethroid-Resistant <i>Aedes aegypti</i> (L.) (Diptera: Culicidae) |
| title_fullStr | Transfluthrin and Metofluthrin as Effective Repellents against Pyrethroid-Susceptible and Pyrethroid-Resistant <i>Aedes aegypti</i> (L.) (Diptera: Culicidae) |
| title_full_unstemmed | Transfluthrin and Metofluthrin as Effective Repellents against Pyrethroid-Susceptible and Pyrethroid-Resistant <i>Aedes aegypti</i> (L.) (Diptera: Culicidae) |
| title_short | Transfluthrin and Metofluthrin as Effective Repellents against Pyrethroid-Susceptible and Pyrethroid-Resistant <i>Aedes aegypti</i> (L.) (Diptera: Culicidae) |
| title_sort | transfluthrin and metofluthrin as effective repellents against pyrethroid susceptible and pyrethroid resistant i aedes aegypti i l diptera culicidae |
| topic | <i>Aedes aegypti</i> spatial repellent high-throughput screening system toxicity bioassay transfluthrin metofluthrin |
| url | https://www.mdpi.com/2075-4450/14/9/767 |
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