The effect of a DNA damaging agent on embryonic cell cycles of the cnidarian Hydractinia echinata.
The onset of gastrulation at the Mid-Blastula Transition can accompany profound changes in embryonic cell cycles including the introduction of gap phases and the transition from maternal to zygotic control. Studies in Xenopus and Drosophila embryos have also found that cell cycles respond to DNA dam...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2010-07-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC2909257?pdf=render |
| _version_ | 1818197496066736128 |
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| author | Tin Tin Su |
| author_facet | Tin Tin Su |
| author_sort | Tin Tin Su |
| collection | DOAJ |
| description | The onset of gastrulation at the Mid-Blastula Transition can accompany profound changes in embryonic cell cycles including the introduction of gap phases and the transition from maternal to zygotic control. Studies in Xenopus and Drosophila embryos have also found that cell cycles respond to DNA damage differently before and after MBT (or its equivalent, MZT, in Drosophila). DNA checkpoints are absent in Xenopus cleavage cycles but are acquired during MBT. Drosophila cleavage nuclei enter an abortive mitosis in the presence of DNA damage whereas post-MZT cells delay the entry into mitosis. Despite attributes that render them workhorses of embryonic cell cycle studies, Xenopus and Drosophila are hardly representative of diverse animal forms that exist. To investigate developmental changes in DNA damage responses in a distant phylum, I studied the effect of an alkylating agent, Methyl Methanesulfonate (MMS), on embryos of Hydractinia echinata. Hydractinia embryos are found to differ from Xenopus embryos in the ability to respond to a DNA damaging agent in early cleavage but are similar to Xenopus and Drosophila embryos in acquiring stronger DNA damage responses and greater resistance to killing by MMS after the onset of gastrulation. This represents the first study of DNA damage responses in the phylum Cnidaria. |
| first_indexed | 2024-12-12T01:50:54Z |
| format | Article |
| id | doaj.art-e0caadda20bb44b4ae6503f2a4b3d688 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-12T01:50:54Z |
| publishDate | 2010-07-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-e0caadda20bb44b4ae6503f2a4b3d6882022-12-22T00:42:30ZengPublic Library of Science (PLoS)PLoS ONE1932-62032010-07-0157e1176010.1371/journal.pone.0011760The effect of a DNA damaging agent on embryonic cell cycles of the cnidarian Hydractinia echinata.Tin Tin SuThe onset of gastrulation at the Mid-Blastula Transition can accompany profound changes in embryonic cell cycles including the introduction of gap phases and the transition from maternal to zygotic control. Studies in Xenopus and Drosophila embryos have also found that cell cycles respond to DNA damage differently before and after MBT (or its equivalent, MZT, in Drosophila). DNA checkpoints are absent in Xenopus cleavage cycles but are acquired during MBT. Drosophila cleavage nuclei enter an abortive mitosis in the presence of DNA damage whereas post-MZT cells delay the entry into mitosis. Despite attributes that render them workhorses of embryonic cell cycle studies, Xenopus and Drosophila are hardly representative of diverse animal forms that exist. To investigate developmental changes in DNA damage responses in a distant phylum, I studied the effect of an alkylating agent, Methyl Methanesulfonate (MMS), on embryos of Hydractinia echinata. Hydractinia embryos are found to differ from Xenopus embryos in the ability to respond to a DNA damaging agent in early cleavage but are similar to Xenopus and Drosophila embryos in acquiring stronger DNA damage responses and greater resistance to killing by MMS after the onset of gastrulation. This represents the first study of DNA damage responses in the phylum Cnidaria.http://europepmc.org/articles/PMC2909257?pdf=render |
| spellingShingle | Tin Tin Su The effect of a DNA damaging agent on embryonic cell cycles of the cnidarian Hydractinia echinata. PLoS ONE |
| title | The effect of a DNA damaging agent on embryonic cell cycles of the cnidarian Hydractinia echinata. |
| title_full | The effect of a DNA damaging agent on embryonic cell cycles of the cnidarian Hydractinia echinata. |
| title_fullStr | The effect of a DNA damaging agent on embryonic cell cycles of the cnidarian Hydractinia echinata. |
| title_full_unstemmed | The effect of a DNA damaging agent on embryonic cell cycles of the cnidarian Hydractinia echinata. |
| title_short | The effect of a DNA damaging agent on embryonic cell cycles of the cnidarian Hydractinia echinata. |
| title_sort | effect of a dna damaging agent on embryonic cell cycles of the cnidarian hydractinia echinata |
| url | http://europepmc.org/articles/PMC2909257?pdf=render |
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