Differential sprouting ability during micropropagation of Nidularium minutum Mez (Bromeliaceae)
Nidularium minutum is an ornamental bromeliad from the Brazilian Rainforest. The micropropagation of this species is essential for obtaining plants available for conservation programs or commercial use. Our study aimed to establish an efficient plant production method by in vitro sprouting. This br...
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| Format: | Article |
| Language: | English |
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Universidade Estadual de Maringá
2023-07-01
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| Series: | Acta Scientiarum: Biological Sciences |
| Subjects: | |
| Online Access: | https://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/64678 |
| _version_ | 1797771123423707136 |
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| author | Maria Gessi Teixeira Catarina Carvalho Nievola |
| author_facet | Maria Gessi Teixeira Catarina Carvalho Nievola |
| author_sort | Maria Gessi Teixeira |
| collection | DOAJ |
| description |
Nidularium minutum is an ornamental bromeliad from the Brazilian Rainforest. The micropropagation of this species is essential for obtaining plants available for conservation programs or commercial use. Our study aimed to establish an efficient plant production method by in vitro sprouting. This bromeliad takes a long time to sprout in vitro, and 10% of the plants produce shoots in a culture medium without plant growth regulators (PGRs). When subcultured in a PGR-free medium, these individualized shoots can sprout like the mother plant. The Murashige and Skoog basal medium (MS) containing 1.0 mg L-1 of 6-benzylaminopurine (BAP) promoted the induction of adventitious shoots in greater than 90% of the plants after 240 days of culture with an average of more than eight shoots per plant. Approximately 100% of the in vitro-produced shoots survived after acclimatization, reaching the flowering stage. Therefore, our results showed that in vitro regeneration of N. minutum depends on the cultivation period and that plants with a higher sprouting capacity can be selected and used as micropropagation matrices, contributing to the production of this endangered bromeliad.
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| first_indexed | 2024-03-12T21:31:59Z |
| format | Article |
| id | doaj.art-e0cbc7e80d194f7a92faccd1ffe717d1 |
| institution | Directory Open Access Journal |
| issn | 1679-9283 1807-863X |
| language | English |
| last_indexed | 2024-03-12T21:31:59Z |
| publishDate | 2023-07-01 |
| publisher | Universidade Estadual de Maringá |
| record_format | Article |
| series | Acta Scientiarum: Biological Sciences |
| spelling | doaj.art-e0cbc7e80d194f7a92faccd1ffe717d12023-07-27T17:54:54ZengUniversidade Estadual de MaringáActa Scientiarum: Biological Sciences1679-92831807-863X2023-07-0145110.4025/actascibiolsci.v45i1.64678Differential sprouting ability during micropropagation of Nidularium minutum Mez (Bromeliaceae)Maria Gessi Teixeira0Catarina Carvalho Nievola1Instituto Federal de Educação, Ciência e Tecnologia do Sul de Minas Gerais / Instituto de Pesquisas AmbientaisInstituto de Pesquisas Ambientais Nidularium minutum is an ornamental bromeliad from the Brazilian Rainforest. The micropropagation of this species is essential for obtaining plants available for conservation programs or commercial use. Our study aimed to establish an efficient plant production method by in vitro sprouting. This bromeliad takes a long time to sprout in vitro, and 10% of the plants produce shoots in a culture medium without plant growth regulators (PGRs). When subcultured in a PGR-free medium, these individualized shoots can sprout like the mother plant. The Murashige and Skoog basal medium (MS) containing 1.0 mg L-1 of 6-benzylaminopurine (BAP) promoted the induction of adventitious shoots in greater than 90% of the plants after 240 days of culture with an average of more than eight shoots per plant. Approximately 100% of the in vitro-produced shoots survived after acclimatization, reaching the flowering stage. Therefore, our results showed that in vitro regeneration of N. minutum depends on the cultivation period and that plants with a higher sprouting capacity can be selected and used as micropropagation matrices, contributing to the production of this endangered bromeliad. https://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/64678bromeliad; endangered species; ornamental plant; shooting; plant regeneration. |
| spellingShingle | Maria Gessi Teixeira Catarina Carvalho Nievola Differential sprouting ability during micropropagation of Nidularium minutum Mez (Bromeliaceae) Acta Scientiarum: Biological Sciences bromeliad; endangered species; ornamental plant; shooting; plant regeneration. |
| title | Differential sprouting ability during micropropagation of Nidularium minutum Mez (Bromeliaceae) |
| title_full | Differential sprouting ability during micropropagation of Nidularium minutum Mez (Bromeliaceae) |
| title_fullStr | Differential sprouting ability during micropropagation of Nidularium minutum Mez (Bromeliaceae) |
| title_full_unstemmed | Differential sprouting ability during micropropagation of Nidularium minutum Mez (Bromeliaceae) |
| title_short | Differential sprouting ability during micropropagation of Nidularium minutum Mez (Bromeliaceae) |
| title_sort | differential sprouting ability during micropropagation of nidularium minutum mez bromeliaceae |
| topic | bromeliad; endangered species; ornamental plant; shooting; plant regeneration. |
| url | https://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/64678 |
| work_keys_str_mv | AT mariagessiteixeira differentialsproutingabilityduringmicropropagationofnidulariumminutummezbromeliaceae AT catarinacarvalhonievola differentialsproutingabilityduringmicropropagationofnidulariumminutummezbromeliaceae |