Polyalthic Acid from <i>Copaifera lucens</i> Demonstrates Anticariogenic and Antiparasitic Properties for Safe Use
This study aimed at evaluating the potential of <i>Copaifera lucens</i>, specifically its oleoresin (CLO), extract (CECL), and the compound <i>ent</i>-polyalthic acid (PA), in combating caries and toxoplasmosis, while also assessing its toxicity. The study involved multiple a...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-09-01
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| Series: | Pharmaceuticals |
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| Online Access: | https://www.mdpi.com/1424-8247/16/10/1357 |
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| author | Mariana B. Santiago Vinicius Cristian O. dos Santos Samuel C. Teixeira Nagela B. S. Silva Pollyanna F. de Oliveira Saulo D. Ozelin Ricardo A. Furtado Denise C. Tavares Sergio Ricardo Ambrósio Rodrigo Cassio S. Veneziani Eloisa Amália V. Ferro Jairo K. Bastos Carlos Henrique G. Martins |
| author_facet | Mariana B. Santiago Vinicius Cristian O. dos Santos Samuel C. Teixeira Nagela B. S. Silva Pollyanna F. de Oliveira Saulo D. Ozelin Ricardo A. Furtado Denise C. Tavares Sergio Ricardo Ambrósio Rodrigo Cassio S. Veneziani Eloisa Amália V. Ferro Jairo K. Bastos Carlos Henrique G. Martins |
| author_sort | Mariana B. Santiago |
| collection | DOAJ |
| description | This study aimed at evaluating the potential of <i>Copaifera lucens</i>, specifically its oleoresin (CLO), extract (CECL), and the compound <i>ent</i>-polyalthic acid (PA), in combating caries and toxoplasmosis, while also assessing its toxicity. The study involved multiple assessments, including determining the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against cariogenic bacteria. CLO and PA exhibited MIC and MBC values ranging from 25 to 50 μg/mL, whereas CECL showed values equal to or exceeding 400 μg/mL. PA also displayed antibiofilm activity with minimum inhibitory concentration of biofilm (MICB<sub>50</sub>) values spanning from 62.5 to 1000 μg/mL. Moreover, PA effectively hindered the intracellular proliferation of <i>Toxoplasma gondii</i> at 64 μg/mL, even after 24 h without treatment. Toxicological evaluations included in vitro tests on V79 cells, where concentrations ranged from 78.1 to 1250 μg/mL of PA reduced colony formation. Additionally, using the <i>Caenorhabditis elegans</i> model, the lethal concentration (LC<sub>50</sub>) of PA was determined as 1000 μg/mL after 48 h of incubation. Notably, no significant differences in micronucleus induction and the NDI were observed in cultures treated with 10, 20, or 40 μg/mL of CLO. These findings underscore the safety profile of CLO and PA, highlighting their potential as alternative treatments for caries and toxoplasmosis. |
| first_indexed | 2024-03-10T20:59:08Z |
| format | Article |
| id | doaj.art-e0cf14e1a7f9470c85728b3b220e2270 |
| institution | Directory Open Access Journal |
| issn | 1424-8247 |
| language | English |
| last_indexed | 2024-03-10T20:59:08Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj.art-e0cf14e1a7f9470c85728b3b220e22702023-11-19T17:41:19ZengMDPI AGPharmaceuticals1424-82472023-09-011610135710.3390/ph16101357Polyalthic Acid from <i>Copaifera lucens</i> Demonstrates Anticariogenic and Antiparasitic Properties for Safe UseMariana B. Santiago0Vinicius Cristian O. dos Santos1Samuel C. Teixeira2Nagela B. S. Silva3Pollyanna F. de Oliveira4Saulo D. Ozelin5Ricardo A. Furtado6Denise C. Tavares7Sergio Ricardo Ambrósio8Rodrigo Cassio S. Veneziani9Eloisa Amália V. Ferro10Jairo K. Bastos11Carlos Henrique G. Martins12Laboratory of Antimicrobial Testing, Institute of Biomedical Sciences, Federal University of Uberlândia, Uberlândia 38405318, MG, BrazilLaboratory of Antimicrobial Testing, Institute of Biomedical Sciences, Federal University of Uberlândia, Uberlândia 38405318, MG, BrazilLaboratory of Immunophysiology of Reproduction, Institute of Biomedical Science, Federal University of Uberlândia, Uberlândia 38405318, MG, BrazilLaboratory of Antimicrobial Testing, Institute of Biomedical Sciences, Federal University of Uberlândia, Uberlândia 38405318, MG, BrazilNucleus of Research in Sciences and Technology, University of Franca, Franca 14404600, SP, BrazilNucleus of Research in Sciences and Technology, University of Franca, Franca 14404600, SP, BrazilNucleus of Research in Sciences and Technology, University of Franca, Franca 14404600, SP, BrazilNucleus of Research in Sciences and Technology, University of Franca, Franca 14404600, SP, BrazilNucleus of Research in Sciences and Technology, University of Franca, Franca 14404600, SP, BrazilNucleus of Research in Sciences and Technology, University of Franca, Franca 14404600, SP, BrazilLaboratory of Immunophysiology of Reproduction, Institute of Biomedical Science, Federal University of Uberlândia, Uberlândia 38405318, MG, BrazilFaculty of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto 14040900, SP, BrazilLaboratory of Antimicrobial Testing, Institute of Biomedical Sciences, Federal University of Uberlândia, Uberlândia 38405318, MG, BrazilThis study aimed at evaluating the potential of <i>Copaifera lucens</i>, specifically its oleoresin (CLO), extract (CECL), and the compound <i>ent</i>-polyalthic acid (PA), in combating caries and toxoplasmosis, while also assessing its toxicity. The study involved multiple assessments, including determining the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against cariogenic bacteria. CLO and PA exhibited MIC and MBC values ranging from 25 to 50 μg/mL, whereas CECL showed values equal to or exceeding 400 μg/mL. PA also displayed antibiofilm activity with minimum inhibitory concentration of biofilm (MICB<sub>50</sub>) values spanning from 62.5 to 1000 μg/mL. Moreover, PA effectively hindered the intracellular proliferation of <i>Toxoplasma gondii</i> at 64 μg/mL, even after 24 h without treatment. Toxicological evaluations included in vitro tests on V79 cells, where concentrations ranged from 78.1 to 1250 μg/mL of PA reduced colony formation. Additionally, using the <i>Caenorhabditis elegans</i> model, the lethal concentration (LC<sub>50</sub>) of PA was determined as 1000 μg/mL after 48 h of incubation. Notably, no significant differences in micronucleus induction and the NDI were observed in cultures treated with 10, 20, or 40 μg/mL of CLO. These findings underscore the safety profile of CLO and PA, highlighting their potential as alternative treatments for caries and toxoplasmosis.https://www.mdpi.com/1424-8247/16/10/1357<i>Copaifera lucens</i><i>ent</i>-polyalthic acidantibacterialantibiofilmantiparasitictoxicity |
| spellingShingle | Mariana B. Santiago Vinicius Cristian O. dos Santos Samuel C. Teixeira Nagela B. S. Silva Pollyanna F. de Oliveira Saulo D. Ozelin Ricardo A. Furtado Denise C. Tavares Sergio Ricardo Ambrósio Rodrigo Cassio S. Veneziani Eloisa Amália V. Ferro Jairo K. Bastos Carlos Henrique G. Martins Polyalthic Acid from <i>Copaifera lucens</i> Demonstrates Anticariogenic and Antiparasitic Properties for Safe Use Pharmaceuticals <i>Copaifera lucens</i> <i>ent</i>-polyalthic acid antibacterial antibiofilm antiparasitic toxicity |
| title | Polyalthic Acid from <i>Copaifera lucens</i> Demonstrates Anticariogenic and Antiparasitic Properties for Safe Use |
| title_full | Polyalthic Acid from <i>Copaifera lucens</i> Demonstrates Anticariogenic and Antiparasitic Properties for Safe Use |
| title_fullStr | Polyalthic Acid from <i>Copaifera lucens</i> Demonstrates Anticariogenic and Antiparasitic Properties for Safe Use |
| title_full_unstemmed | Polyalthic Acid from <i>Copaifera lucens</i> Demonstrates Anticariogenic and Antiparasitic Properties for Safe Use |
| title_short | Polyalthic Acid from <i>Copaifera lucens</i> Demonstrates Anticariogenic and Antiparasitic Properties for Safe Use |
| title_sort | polyalthic acid from i copaifera lucens i demonstrates anticariogenic and antiparasitic properties for safe use |
| topic | <i>Copaifera lucens</i> <i>ent</i>-polyalthic acid antibacterial antibiofilm antiparasitic toxicity |
| url | https://www.mdpi.com/1424-8247/16/10/1357 |
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