Combined Effect of Plasma-Activated Water and Topotecan in Glioblastoma Cells
The increase in cancer diagnoses and cancer deaths, severe side effects of existing treatments and resistance to traditional treatments have generated a need for new anticancer treatments. Glioblastoma multiforme (GBM) is the most common, malignant and aggressive brain cancer. Despite many innovatio...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-10-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/15/19/4858 |
| _version_ | 1797576145015668736 |
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| author | Beatriz Pinheiro Lopes Liam O’Neill Paula Bourke Daniela Boehm |
| author_facet | Beatriz Pinheiro Lopes Liam O’Neill Paula Bourke Daniela Boehm |
| author_sort | Beatriz Pinheiro Lopes |
| collection | DOAJ |
| description | The increase in cancer diagnoses and cancer deaths, severe side effects of existing treatments and resistance to traditional treatments have generated a need for new anticancer treatments. Glioblastoma multiforme (GBM) is the most common, malignant and aggressive brain cancer. Despite many innovations regarding GBM treatment, the final outcome is still very poor, making it necessary to develop new therapeutic approaches. Cold atmospheric plasma (CAP) as well as plasma-activated liquids (PAL) are being studied as new possible approaches against cancer. The anticancer activity of PAL such as “plasma-activated water” (PAW) is dependent on the reactive chemical compounds present in the solution. Possible combinatory effects with conventional therapies, such as chemotherapeutics, may expand the potential of PAL for cancer treatment. We aim to explore the therapeutic properties of a combination of PAW and topotecan (TPT), an antineoplastic agent with major cytotoxic effects during the S phase of the cell cycle, on a GBM cancer cell line (U-251mg). Combined treatments with PAW and TPT showed a reduction in the metabolic activity and cell mass, an increase in apoptotic cell death and a reduction in the long-term survival. Single applications of PAW+TPT treatments showed a cytotoxic effect in the short term and an antiproliferative effect in the long term, warranting future exploration of combining PAW with chemotherapeutic agents as new therapeutic approaches. |
| first_indexed | 2024-03-10T21:48:06Z |
| format | Article |
| id | doaj.art-e0cf2c6ce17245dcb421152789acab44 |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-10T21:48:06Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-e0cf2c6ce17245dcb421152789acab442023-11-19T14:11:35ZengMDPI AGCancers2072-66942023-10-011519485810.3390/cancers15194858Combined Effect of Plasma-Activated Water and Topotecan in Glioblastoma CellsBeatriz Pinheiro Lopes0Liam O’Neill1Paula Bourke2Daniela Boehm3School of Chemical and Bioprocess Engineering, University College Dublin, D04 V1W8 Dublin, IrelandTheraDep Ltd., QUESTUM Innovation Centre, Limerick Institute of Technology, E91 V329 Clonmel, IrelandEnvironmental Sustainability and Health Institute and School of Food Science and Environmental Health, Technological University Dublin, D07 H6K8 Dublin, IrelandSchool of Chemical and Bioprocess Engineering, University College Dublin, D04 V1W8 Dublin, IrelandThe increase in cancer diagnoses and cancer deaths, severe side effects of existing treatments and resistance to traditional treatments have generated a need for new anticancer treatments. Glioblastoma multiforme (GBM) is the most common, malignant and aggressive brain cancer. Despite many innovations regarding GBM treatment, the final outcome is still very poor, making it necessary to develop new therapeutic approaches. Cold atmospheric plasma (CAP) as well as plasma-activated liquids (PAL) are being studied as new possible approaches against cancer. The anticancer activity of PAL such as “plasma-activated water” (PAW) is dependent on the reactive chemical compounds present in the solution. Possible combinatory effects with conventional therapies, such as chemotherapeutics, may expand the potential of PAL for cancer treatment. We aim to explore the therapeutic properties of a combination of PAW and topotecan (TPT), an antineoplastic agent with major cytotoxic effects during the S phase of the cell cycle, on a GBM cancer cell line (U-251mg). Combined treatments with PAW and TPT showed a reduction in the metabolic activity and cell mass, an increase in apoptotic cell death and a reduction in the long-term survival. Single applications of PAW+TPT treatments showed a cytotoxic effect in the short term and an antiproliferative effect in the long term, warranting future exploration of combining PAW with chemotherapeutic agents as new therapeutic approaches.https://www.mdpi.com/2072-6694/15/19/4858glioblastomaanticancer treatmentplasma-activated waterreactive speciestopotecancombination treatments |
| spellingShingle | Beatriz Pinheiro Lopes Liam O’Neill Paula Bourke Daniela Boehm Combined Effect of Plasma-Activated Water and Topotecan in Glioblastoma Cells Cancers glioblastoma anticancer treatment plasma-activated water reactive species topotecan combination treatments |
| title | Combined Effect of Plasma-Activated Water and Topotecan in Glioblastoma Cells |
| title_full | Combined Effect of Plasma-Activated Water and Topotecan in Glioblastoma Cells |
| title_fullStr | Combined Effect of Plasma-Activated Water and Topotecan in Glioblastoma Cells |
| title_full_unstemmed | Combined Effect of Plasma-Activated Water and Topotecan in Glioblastoma Cells |
| title_short | Combined Effect of Plasma-Activated Water and Topotecan in Glioblastoma Cells |
| title_sort | combined effect of plasma activated water and topotecan in glioblastoma cells |
| topic | glioblastoma anticancer treatment plasma-activated water reactive species topotecan combination treatments |
| url | https://www.mdpi.com/2072-6694/15/19/4858 |
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