2Long-read sequencing revealed alterations of microbial relationship between tongue coating and gastric mucosa in patients with gastric intestinal metaplasia
Objective: To explore the microbial correlation between oral tongue coating (TC) and gastric mucosa (GM) in patients with gastric intestinal metaplasia (GIM). Methods: The present study recruited 1360 volunteers for upper gastrointestinal cancer screening. The microbiota in TC and GM were profiled b...
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| Format: | Article |
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KeAi Communications Co., Ltd.
2023-12-01
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| Series: | Digital Chinese Medicine |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589377724000077 |
| _version_ | 1797319447139057664 |
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| author | Wu Jianping Li Meifeng Hua Zhaolai Lu Bin Xiang Jiao Wu Zhenfeng Zhang Junfeng |
| author_facet | Wu Jianping Li Meifeng Hua Zhaolai Lu Bin Xiang Jiao Wu Zhenfeng Zhang Junfeng |
| author_sort | Wu Jianping |
| collection | DOAJ |
| description | Objective: To explore the microbial correlation between oral tongue coating (TC) and gastric mucosa (GM) in patients with gastric intestinal metaplasia (GIM). Methods: The present study recruited 1360 volunteers for upper gastrointestinal cancer screening. The microbiota in TC and GM were profiled by long-read sequencing of full-length 16S rRNA gene. The microbial diversity, community structure, and linear discriminant analysis effect size (LEfSe) were analyzed by the software Visual Genomics. SparCC correlation analysis was used to construct the commensal network and the graphical display was conducted by R software. Results: The population included 44 patients with precancerous GIM, and 28 matched controls with negative rapid urease test (RUT) and non-symptomatic chronic superficial gastritis (CSG). No significant difference in diversity was observed between GIM patients and controls in TC or GM microbiota (P > 0.05). Patients had a higher percentage of 41 – 60 co-occurring operational taxonomic units (OTUs) between TC and GM than controls (34.1 % vs. 25.0 %) (P < 0.05). The LEfSe showed that TC Prevotella melaninogenica and three gastric Helicobacter species (i.e., Helicobacter pylori, Helicobacter pylori XZ274, and Helicobacter pylori 83) were enriched in patients with GIM. Furthermore, GIM patients with positive RUT had a lower percentage of co-occurring OTUs over 20 (P < 0.05), and lower abundances of gastric Veillonella, Pseudonocardia, and Mesorhizobium than those with negative RUT (P < 0.05). The commensal network between TC and GM was more complex in GIM patients than in controls. GIM patients with positive RUT demonstrated more bacterial correlations between TC and GM than those with negative RUT. Finally, the serum ratio of PG-I/II was negatively correlated with three gastric Helicobacter species (Helicobacter pylori, Helicobacter pylori XZ274, and Helicobacter pylori 83) in patients with negative RUT (P < 0.05), and negatively correlated with two TC species (Fusobacterium nucleatum subsp. nucleatum and Campylobacter showae) in patients with positive RUT (P < 0.05). Conclusion: The development of GIM potentiated the commensal network between oral TC and GM, providing microbial evidence of the correlation between TC and the stomach. |
| first_indexed | 2024-03-08T04:07:01Z |
| format | Article |
| id | doaj.art-e0d0d34efd6e4fe1a9ff363a2d310d3e |
| institution | Directory Open Access Journal |
| issn | 2589-3777 |
| language | English |
| last_indexed | 2024-03-08T04:07:01Z |
| publishDate | 2023-12-01 |
| publisher | KeAi Communications Co., Ltd. |
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| series | Digital Chinese Medicine |
| spelling | doaj.art-e0d0d34efd6e4fe1a9ff363a2d310d3e2024-02-09T04:48:59ZengKeAi Communications Co., Ltd.Digital Chinese Medicine2589-37772023-12-01644384502Long-read sequencing revealed alterations of microbial relationship between tongue coating and gastric mucosa in patients with gastric intestinal metaplasiaWu Jianping0Li Meifeng1Hua Zhaolai2Lu Bin3Xiang Jiao4Wu Zhenfeng5Zhang Junfeng6School of Medicine, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, ChinaSchool of Medicine, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, ChinaInstitute of Tumour Prevention and Control, People's Hospital of Yangzhong City, Yangzhong, Jiangsu 212299, ChinaDepartment of Oncology, People's Hospital of Yangzhong City, Yangzhong, Jiangsu 212299, ChinaSchool of Medicine, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, ChinaDepartment of Surgical Oncology, Jiangsu Province Hospital of Chinese Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210029, China; Corresponding author:School of Medicine, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, China; Corresponding author:Objective: To explore the microbial correlation between oral tongue coating (TC) and gastric mucosa (GM) in patients with gastric intestinal metaplasia (GIM). Methods: The present study recruited 1360 volunteers for upper gastrointestinal cancer screening. The microbiota in TC and GM were profiled by long-read sequencing of full-length 16S rRNA gene. The microbial diversity, community structure, and linear discriminant analysis effect size (LEfSe) were analyzed by the software Visual Genomics. SparCC correlation analysis was used to construct the commensal network and the graphical display was conducted by R software. Results: The population included 44 patients with precancerous GIM, and 28 matched controls with negative rapid urease test (RUT) and non-symptomatic chronic superficial gastritis (CSG). No significant difference in diversity was observed between GIM patients and controls in TC or GM microbiota (P > 0.05). Patients had a higher percentage of 41 – 60 co-occurring operational taxonomic units (OTUs) between TC and GM than controls (34.1 % vs. 25.0 %) (P < 0.05). The LEfSe showed that TC Prevotella melaninogenica and three gastric Helicobacter species (i.e., Helicobacter pylori, Helicobacter pylori XZ274, and Helicobacter pylori 83) were enriched in patients with GIM. Furthermore, GIM patients with positive RUT had a lower percentage of co-occurring OTUs over 20 (P < 0.05), and lower abundances of gastric Veillonella, Pseudonocardia, and Mesorhizobium than those with negative RUT (P < 0.05). The commensal network between TC and GM was more complex in GIM patients than in controls. GIM patients with positive RUT demonstrated more bacterial correlations between TC and GM than those with negative RUT. Finally, the serum ratio of PG-I/II was negatively correlated with three gastric Helicobacter species (Helicobacter pylori, Helicobacter pylori XZ274, and Helicobacter pylori 83) in patients with negative RUT (P < 0.05), and negatively correlated with two TC species (Fusobacterium nucleatum subsp. nucleatum and Campylobacter showae) in patients with positive RUT (P < 0.05). Conclusion: The development of GIM potentiated the commensal network between oral TC and GM, providing microbial evidence of the correlation between TC and the stomach.http://www.sciencedirect.com/science/article/pii/S2589377724000077Gastric intestinal metaplasia (GIM)Tongue coating (TC)Gastric mucosa (GM)MicrobiotaCommensal network |
| spellingShingle | Wu Jianping Li Meifeng Hua Zhaolai Lu Bin Xiang Jiao Wu Zhenfeng Zhang Junfeng 2Long-read sequencing revealed alterations of microbial relationship between tongue coating and gastric mucosa in patients with gastric intestinal metaplasia Digital Chinese Medicine Gastric intestinal metaplasia (GIM) Tongue coating (TC) Gastric mucosa (GM) Microbiota Commensal network |
| title | 2Long-read sequencing revealed alterations of microbial relationship between tongue coating and gastric mucosa in patients with gastric intestinal metaplasia |
| title_full | 2Long-read sequencing revealed alterations of microbial relationship between tongue coating and gastric mucosa in patients with gastric intestinal metaplasia |
| title_fullStr | 2Long-read sequencing revealed alterations of microbial relationship between tongue coating and gastric mucosa in patients with gastric intestinal metaplasia |
| title_full_unstemmed | 2Long-read sequencing revealed alterations of microbial relationship between tongue coating and gastric mucosa in patients with gastric intestinal metaplasia |
| title_short | 2Long-read sequencing revealed alterations of microbial relationship between tongue coating and gastric mucosa in patients with gastric intestinal metaplasia |
| title_sort | 2long read sequencing revealed alterations of microbial relationship between tongue coating and gastric mucosa in patients with gastric intestinal metaplasia |
| topic | Gastric intestinal metaplasia (GIM) Tongue coating (TC) Gastric mucosa (GM) Microbiota Commensal network |
| url | http://www.sciencedirect.com/science/article/pii/S2589377724000077 |
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