Utilisation of Quartz Crystal Microbalance Sensors with Dissipation (QCM-D) for a Clauss Fibrinogen Assay in Comparison with Common Coagulation Reference Methods
The determination of fibrinogen levels is one of the most important coagulation measurements in medicine. It plays a crucial part in diagnostic and therapeutic decisions, often associated with time-critical conditions. The commonly used measurement is the Clauss fibrinogen assay (CFA) where plasma i...
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MDPI AG
2016-02-01
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| Series: | Sensors |
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| Online Access: | http://www.mdpi.com/1424-8220/16/3/282 |
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| author | Stephanie Oberfrank Hartmut Drechsel Stefan Sinn Hinnak Northoff Frank K. Gehring |
| author_facet | Stephanie Oberfrank Hartmut Drechsel Stefan Sinn Hinnak Northoff Frank K. Gehring |
| author_sort | Stephanie Oberfrank |
| collection | DOAJ |
| description | The determination of fibrinogen levels is one of the most important coagulation measurements in medicine. It plays a crucial part in diagnostic and therapeutic decisions, often associated with time-critical conditions. The commonly used measurement is the Clauss fibrinogen assay (CFA) where plasma is activated by thrombin reagent and which is conducted by mechanical/turbidimetric devices. As quartz crystal microbalance sensors with dissipation (QCM-D) based devices have a small footprint, can be operated easily and allow measurements independently from sample transportation time, laboratory location, availability and opening hours, they offer a great opportunity to complement laboratory CFA measurements. Therefore, the objective of the work was to (1) transfer the CFA to the QCM-D method; (2) develop an easy, time- and cost-effective procedure and (3) compare the results with references. Different sensor coatings (donor’s own plasma; gold surface) and different QCM-D parameters (frequency signal shift; its calculated turning point; dissipation signal shift) were sampled. The results demonstrate the suitability for a QCM-D-based CFA in physiological fibrinogen ranges. Results were obtained in less than 1 min and in very good agreement with a standardized reference (Merlin coagulometer). The results provide a good basis for further investigation and pave the way to a possible application of QCM-D in clinical and non-clinical routine in the medical field. |
| first_indexed | 2024-04-11T14:02:00Z |
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| institution | Directory Open Access Journal |
| issn | 1424-8220 |
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| last_indexed | 2024-04-11T14:02:00Z |
| publishDate | 2016-02-01 |
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| spelling | doaj.art-e0d12252331746c49d0e87594a7c4b992022-12-22T04:20:06ZengMDPI AGSensors1424-82202016-02-0116328210.3390/s16030282s16030282Utilisation of Quartz Crystal Microbalance Sensors with Dissipation (QCM-D) for a Clauss Fibrinogen Assay in Comparison with Common Coagulation Reference MethodsStephanie Oberfrank0Hartmut Drechsel1Stefan Sinn2Hinnak Northoff3Frank K. Gehring4Biosensorik-Gruppe, Institut für Klinische und Experimentelle Transfusionsmedizin (IKET), Universitätsklinikum Tübingen, Otfried-Müller-Str. 27, 72076 Tübingen, GermanyBiosensorik-Gruppe, Institut für Klinische und Experimentelle Transfusionsmedizin (IKET), Universitätsklinikum Tübingen, Otfried-Müller-Str. 27, 72076 Tübingen, GermanyBiosensorik-Gruppe, Institut für Klinische und Experimentelle Transfusionsmedizin (IKET), Universitätsklinikum Tübingen, Otfried-Müller-Str. 27, 72076 Tübingen, GermanyBiosensorik-Gruppe, Institut für Klinische und Experimentelle Transfusionsmedizin (IKET), Universitätsklinikum Tübingen, Otfried-Müller-Str. 27, 72076 Tübingen, GermanyBiosensorik-Gruppe, Institut für Klinische und Experimentelle Transfusionsmedizin (IKET), Universitätsklinikum Tübingen, Otfried-Müller-Str. 27, 72076 Tübingen, GermanyThe determination of fibrinogen levels is one of the most important coagulation measurements in medicine. It plays a crucial part in diagnostic and therapeutic decisions, often associated with time-critical conditions. The commonly used measurement is the Clauss fibrinogen assay (CFA) where plasma is activated by thrombin reagent and which is conducted by mechanical/turbidimetric devices. As quartz crystal microbalance sensors with dissipation (QCM-D) based devices have a small footprint, can be operated easily and allow measurements independently from sample transportation time, laboratory location, availability and opening hours, they offer a great opportunity to complement laboratory CFA measurements. Therefore, the objective of the work was to (1) transfer the CFA to the QCM-D method; (2) develop an easy, time- and cost-effective procedure and (3) compare the results with references. Different sensor coatings (donor’s own plasma; gold surface) and different QCM-D parameters (frequency signal shift; its calculated turning point; dissipation signal shift) were sampled. The results demonstrate the suitability for a QCM-D-based CFA in physiological fibrinogen ranges. Results were obtained in less than 1 min and in very good agreement with a standardized reference (Merlin coagulometer). The results provide a good basis for further investigation and pave the way to a possible application of QCM-D in clinical and non-clinical routine in the medical field.http://www.mdpi.com/1424-8220/16/3/282Quartz Crystal Microbalance with dissipation (QCM-D)Clauss Fibrinogen AssayBlood CoagulationBlood ViscoelasticityFibrinogen |
| spellingShingle | Stephanie Oberfrank Hartmut Drechsel Stefan Sinn Hinnak Northoff Frank K. Gehring Utilisation of Quartz Crystal Microbalance Sensors with Dissipation (QCM-D) for a Clauss Fibrinogen Assay in Comparison with Common Coagulation Reference Methods Sensors Quartz Crystal Microbalance with dissipation (QCM-D) Clauss Fibrinogen Assay Blood Coagulation Blood Viscoelasticity Fibrinogen |
| title | Utilisation of Quartz Crystal Microbalance Sensors with Dissipation (QCM-D) for a Clauss Fibrinogen Assay in Comparison with Common Coagulation Reference Methods |
| title_full | Utilisation of Quartz Crystal Microbalance Sensors with Dissipation (QCM-D) for a Clauss Fibrinogen Assay in Comparison with Common Coagulation Reference Methods |
| title_fullStr | Utilisation of Quartz Crystal Microbalance Sensors with Dissipation (QCM-D) for a Clauss Fibrinogen Assay in Comparison with Common Coagulation Reference Methods |
| title_full_unstemmed | Utilisation of Quartz Crystal Microbalance Sensors with Dissipation (QCM-D) for a Clauss Fibrinogen Assay in Comparison with Common Coagulation Reference Methods |
| title_short | Utilisation of Quartz Crystal Microbalance Sensors with Dissipation (QCM-D) for a Clauss Fibrinogen Assay in Comparison with Common Coagulation Reference Methods |
| title_sort | utilisation of quartz crystal microbalance sensors with dissipation qcm d for a clauss fibrinogen assay in comparison with common coagulation reference methods |
| topic | Quartz Crystal Microbalance with dissipation (QCM-D) Clauss Fibrinogen Assay Blood Coagulation Blood Viscoelasticity Fibrinogen |
| url | http://www.mdpi.com/1424-8220/16/3/282 |
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