Modification of Glyceraldehyde-3-Phosphate Dehydrogenase with Nitric Oxide: Role in Signal Transduction and Development of Apoptosis
This review focuses on the consequences of GAPDH <i>S</i>-nitrosylation at the catalytic cysteine residue. The widespread hypothesis according to which <i>S</i>-nitrosylation causes a change in GAPDH structure and its subsequent binding to the Siah1 protein is considered in d...
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| Format: | Article |
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MDPI AG
2021-11-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/11/11/1656 |
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| author | Vladimir I. Muronetz Maria V. Medvedeva Irina A. Sevostyanova Elena V. Schmalhausen |
| author_facet | Vladimir I. Muronetz Maria V. Medvedeva Irina A. Sevostyanova Elena V. Schmalhausen |
| author_sort | Vladimir I. Muronetz |
| collection | DOAJ |
| description | This review focuses on the consequences of GAPDH <i>S</i>-nitrosylation at the catalytic cysteine residue. The widespread hypothesis according to which <i>S</i>-nitrosylation causes a change in GAPDH structure and its subsequent binding to the Siah1 protein is considered in detail. It is assumed that the GAPDH complex with Siah1 is transported to the nucleus by carrier proteins, interacts with nuclear proteins, and induces apoptosis. However, there are several conflicting and unproven elements in this hypothesis. In particular, there is no direct confirmation of the interaction between the tetrameric GAPDH and Siah1 caused by <i>S</i>-nitrosylation of GAPDH. The question remains as to whether the translocation of GAPDH into the nucleus is caused by <i>S</i>-nitrosylation or by some other modification of the catalytic cysteine residue. The hypothesis of the induction of apoptosis by oxidation of GAPDH is considered. This oxidation leads to a release of the coenzyme NAD<sup>+</sup> from the active center of GAPDH, followed by the dissociation of the tetramer into subunits, which move to the nucleus due to passive transport and induce apoptosis. In conclusion, the main tasks are summarized, the solutions to which will make it possible to more definitively establish the role of nitric oxide in the induction of apoptosis. |
| first_indexed | 2024-03-10T05:40:44Z |
| format | Article |
| id | doaj.art-e0d37a8968e44f25839b2c93f5ab8840 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-10T05:40:44Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-e0d37a8968e44f25839b2c93f5ab88402023-11-22T22:34:23ZengMDPI AGBiomolecules2218-273X2021-11-011111165610.3390/biom11111656Modification of Glyceraldehyde-3-Phosphate Dehydrogenase with Nitric Oxide: Role in Signal Transduction and Development of ApoptosisVladimir I. Muronetz0Maria V. Medvedeva1Irina A. Sevostyanova2Elena V. Schmalhausen3Belozersky Institute of Physico Chemical Biology, Lomonosov Moscow State University, 119234 Moscow, RussiaFaculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, 119234 Moscow, RussiaBelozersky Institute of Physico Chemical Biology, Lomonosov Moscow State University, 119234 Moscow, RussiaBelozersky Institute of Physico Chemical Biology, Lomonosov Moscow State University, 119234 Moscow, RussiaThis review focuses on the consequences of GAPDH <i>S</i>-nitrosylation at the catalytic cysteine residue. The widespread hypothesis according to which <i>S</i>-nitrosylation causes a change in GAPDH structure and its subsequent binding to the Siah1 protein is considered in detail. It is assumed that the GAPDH complex with Siah1 is transported to the nucleus by carrier proteins, interacts with nuclear proteins, and induces apoptosis. However, there are several conflicting and unproven elements in this hypothesis. In particular, there is no direct confirmation of the interaction between the tetrameric GAPDH and Siah1 caused by <i>S</i>-nitrosylation of GAPDH. The question remains as to whether the translocation of GAPDH into the nucleus is caused by <i>S</i>-nitrosylation or by some other modification of the catalytic cysteine residue. The hypothesis of the induction of apoptosis by oxidation of GAPDH is considered. This oxidation leads to a release of the coenzyme NAD<sup>+</sup> from the active center of GAPDH, followed by the dissociation of the tetramer into subunits, which move to the nucleus due to passive transport and induce apoptosis. In conclusion, the main tasks are summarized, the solutions to which will make it possible to more definitively establish the role of nitric oxide in the induction of apoptosis.https://www.mdpi.com/2218-273X/11/11/1656glyceraldehyde-3-phosphate dehydrogenase<i>S</i>-nitrosylationprotein–protein interactionsoxidationsulfenic acidNO |
| spellingShingle | Vladimir I. Muronetz Maria V. Medvedeva Irina A. Sevostyanova Elena V. Schmalhausen Modification of Glyceraldehyde-3-Phosphate Dehydrogenase with Nitric Oxide: Role in Signal Transduction and Development of Apoptosis Biomolecules glyceraldehyde-3-phosphate dehydrogenase <i>S</i>-nitrosylation protein–protein interactions oxidation sulfenic acid NO |
| title | Modification of Glyceraldehyde-3-Phosphate Dehydrogenase with Nitric Oxide: Role in Signal Transduction and Development of Apoptosis |
| title_full | Modification of Glyceraldehyde-3-Phosphate Dehydrogenase with Nitric Oxide: Role in Signal Transduction and Development of Apoptosis |
| title_fullStr | Modification of Glyceraldehyde-3-Phosphate Dehydrogenase with Nitric Oxide: Role in Signal Transduction and Development of Apoptosis |
| title_full_unstemmed | Modification of Glyceraldehyde-3-Phosphate Dehydrogenase with Nitric Oxide: Role in Signal Transduction and Development of Apoptosis |
| title_short | Modification of Glyceraldehyde-3-Phosphate Dehydrogenase with Nitric Oxide: Role in Signal Transduction and Development of Apoptosis |
| title_sort | modification of glyceraldehyde 3 phosphate dehydrogenase with nitric oxide role in signal transduction and development of apoptosis |
| topic | glyceraldehyde-3-phosphate dehydrogenase <i>S</i>-nitrosylation protein–protein interactions oxidation sulfenic acid NO |
| url | https://www.mdpi.com/2218-273X/11/11/1656 |
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