Application of Plackett Burman Design for Citric Acid Production from Pretreated and Untreated Wheat Straw
A solid state fermentation method was used to utilize wheat straw as substrates for citric acid production by using Aspergillus niger ATCC 9142. The Plackett Burman design (PBD) of experiments was used to test the relative importance of the variables affecting production such as moisture content, ag...
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| Format: | Article |
| Language: | English |
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Iranian Institute of Research and Development in Chemical Industries (IRDCI)-ACECR
2008-03-01
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| Series: | Iranian Journal of Chemistry & Chemical Engineering |
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| Online Access: | http://www.ijcce.ac.ir/article_7129_0608f08afa7398a38d5a55e784e1738f.pdf |
| _version_ | 1818832154557153280 |
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| author | Kianoosh Khosravi Darani Alale Zoghi Sayed Abolhasan Alavi Sayed Safa Ali Fatemi |
| author_facet | Kianoosh Khosravi Darani Alale Zoghi Sayed Abolhasan Alavi Sayed Safa Ali Fatemi |
| author_sort | Kianoosh Khosravi Darani |
| collection | DOAJ |
| description | A solid state fermentation method was used to utilize wheat straw as substrates for citric acid production by using Aspergillus niger ATCC 9142. The Plackett Burman design (PBD) of experiments was used to test the relative importance of the variables affecting production such as moisture content, age of spore, inoculum size, initial pH of substrate, methanol concentration, incubation temperature and time, as well as initial sugar concentration, steam time and type of solvent. In best condition, the microorganism produced 47 g citric acid per kg dry crude wheat straw, with yield of 93 % based on the amount of fermentable sugar consumed. The effect of pretreatment of wheat straw with HCl, NaOH and urea on the yield of production was also investigated by using PBD. Acid, alkaline and urea pretreatment of wheat straw increased citric acid concentration to 115, 85.0 and 109.5 g/kg of dry wheat straw and yield to 97, 97 and 96 % (respectively) based on available sugar consumed. Finally, up scaling was achieved to a 20-L solid state fermentor (tray bioreactor) in which moisture was constant in gas phase and acid pretreated wheat straw was selected as the most efficient pretreated substrate. The produced acid concentration and yield in fermentor was 60.53 g/kg of dry wheat straw and 2.11 g/kg.day, respectively. |
| first_indexed | 2024-12-19T01:58:31Z |
| format | Article |
| id | doaj.art-e0d392097e824327909f9673c10c2f34 |
| institution | Directory Open Access Journal |
| issn | 1021-9986 1021-9986 |
| language | English |
| last_indexed | 2024-12-19T01:58:31Z |
| publishDate | 2008-03-01 |
| publisher | Iranian Institute of Research and Development in Chemical Industries (IRDCI)-ACECR |
| record_format | Article |
| series | Iranian Journal of Chemistry & Chemical Engineering |
| spelling | doaj.art-e0d392097e824327909f9673c10c2f342022-12-21T20:41:07ZengIranian Institute of Research and Development in Chemical Industries (IRDCI)-ACECRIranian Journal of Chemistry & Chemical Engineering1021-99861021-99862008-03-01271911047129Application of Plackett Burman Design for Citric Acid Production from Pretreated and Untreated Wheat StrawKianoosh Khosravi Darani0Alale Zoghi1Sayed Abolhasan Alavi2Sayed Safa Ali Fatemi3Department of Food Technology Research, National Nutrition and Food Technology Research Institute, Shaheed Beheshti University, M.C., P.O. Box 19395 Tehran, I.R. IRANDepartment of Chemical Engineering, Faculty of Engineering, Azad Islamic University, Science and Technology Campus, P.O. Box 14115-775 Tehran, I.R. IRANDepartment of Chemical Engineering, Faculty of Engineering, Azad Islamic University, Science and Technology Campus, P.O. Box 14115-775 Tehran, I.R. IRANDepartment of Bioprocess Engineering, National Institute for Genetic Engineering and Biotechnology, P.O. Box 14965-161 Tehran, I.R. IRANA solid state fermentation method was used to utilize wheat straw as substrates for citric acid production by using Aspergillus niger ATCC 9142. The Plackett Burman design (PBD) of experiments was used to test the relative importance of the variables affecting production such as moisture content, age of spore, inoculum size, initial pH of substrate, methanol concentration, incubation temperature and time, as well as initial sugar concentration, steam time and type of solvent. In best condition, the microorganism produced 47 g citric acid per kg dry crude wheat straw, with yield of 93 % based on the amount of fermentable sugar consumed. The effect of pretreatment of wheat straw with HCl, NaOH and urea on the yield of production was also investigated by using PBD. Acid, alkaline and urea pretreatment of wheat straw increased citric acid concentration to 115, 85.0 and 109.5 g/kg of dry wheat straw and yield to 97, 97 and 96 % (respectively) based on available sugar consumed. Finally, up scaling was achieved to a 20-L solid state fermentor (tray bioreactor) in which moisture was constant in gas phase and acid pretreated wheat straw was selected as the most efficient pretreated substrate. The produced acid concentration and yield in fermentor was 60.53 g/kg of dry wheat straw and 2.11 g/kg.day, respectively.http://www.ijcce.ac.ir/article_7129_0608f08afa7398a38d5a55e784e1738f.pdfsolid state fermentationcitric acid productionpretreatmentwheat strawplackett-burman design |
| spellingShingle | Kianoosh Khosravi Darani Alale Zoghi Sayed Abolhasan Alavi Sayed Safa Ali Fatemi Application of Plackett Burman Design for Citric Acid Production from Pretreated and Untreated Wheat Straw Iranian Journal of Chemistry & Chemical Engineering solid state fermentation citric acid production pretreatment wheat straw plackett-burman design |
| title | Application of Plackett Burman Design for Citric Acid Production from Pretreated and Untreated Wheat Straw |
| title_full | Application of Plackett Burman Design for Citric Acid Production from Pretreated and Untreated Wheat Straw |
| title_fullStr | Application of Plackett Burman Design for Citric Acid Production from Pretreated and Untreated Wheat Straw |
| title_full_unstemmed | Application of Plackett Burman Design for Citric Acid Production from Pretreated and Untreated Wheat Straw |
| title_short | Application of Plackett Burman Design for Citric Acid Production from Pretreated and Untreated Wheat Straw |
| title_sort | application of plackett burman design for citric acid production from pretreated and untreated wheat straw |
| topic | solid state fermentation citric acid production pretreatment wheat straw plackett-burman design |
| url | http://www.ijcce.ac.ir/article_7129_0608f08afa7398a38d5a55e784e1738f.pdf |
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