FEATURES OF MASS-SPECTROMETRIC PROTEIN PROFILES OF STRAINS OF BRUCELLOSIS CAUSATIVE AGENT DURING PREPARATION OF CULTURE ON VARIOUS NUTRIENT MEDIA
Aim. Carry out comparative analysis using time-of-flight mass-spectrometry with matrix laser desorption/ionization (MALDI-TOF MS) of protein profiles of brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA), cultivated in various nutrient media: Albimi agar, brucellagar...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | Russian |
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Central Research Institute for Epidemiology
2016-02-01
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| Series: | Журнал микробиологии, эпидемиологии и иммунобиологии |
| Subjects: | |
| Online Access: | https://microbiol.elpub.ru/jour/article/view/6 |
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| author | D. V. Ulshina D. A. Kovalev A. M. Zhirov N. V. Zharinova A. A. Khudoleev O. I. Kogotkova V. I. Efremenko N. I. Evchenko A. N. Kulichenko |
| author_facet | D. V. Ulshina D. A. Kovalev A. M. Zhirov N. V. Zharinova A. A. Khudoleev O. I. Kogotkova V. I. Efremenko N. I. Evchenko A. N. Kulichenko |
| author_sort | D. V. Ulshina |
| collection | DOAJ |
| description | Aim. Carry out comparative analysis using time-of-flight mass-spectrometry with matrix laser desorption/ionization (MALDI-TOF MS) of protein profiles of brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA), cultivated in various nutrient media: Albimi agar, brucellagar and erythrit-agar. Materials and methods. Vaccine strains: Brucella melitensis Rev-1 and Brucella abortus 19BA. Protein profiling in linear mode on Microflex «Bruker Daltonics» MALDI-TOF mass-spectrometer. Results. A number of characteristic features of brucella mass-spectra was detected: in particular, preservation of the total qualitative composition of protein profiles of cultures and significant differences in the intensity of separate peaks depending on the nutrient medium used. Conclusion. Based on the analysis of the data obtained, use of Albimi agar as the nutrient medium for preparation of brucella culture samples for mass-spectrometric analysis was shown to be optimal. |
| first_indexed | 2024-04-13T23:34:47Z |
| format | Article |
| id | doaj.art-e0d392760a6044b5b556760ceffec9a4 |
| institution | Directory Open Access Journal |
| issn | 0372-9311 2686-7613 |
| language | Russian |
| last_indexed | 2024-04-13T23:34:47Z |
| publishDate | 2016-02-01 |
| publisher | Central Research Institute for Epidemiology |
| record_format | Article |
| series | Журнал микробиологии, эпидемиологии и иммунобиологии |
| spelling | doaj.art-e0d392760a6044b5b556760ceffec9a42022-12-22T02:24:48ZrusCentral Research Institute for EpidemiologyЖурнал микробиологии, эпидемиологии и иммунобиологии0372-93112686-76132016-02-0101293410.36233/0372-9311-2016-1-29-346FEATURES OF MASS-SPECTROMETRIC PROTEIN PROFILES OF STRAINS OF BRUCELLOSIS CAUSATIVE AGENT DURING PREPARATION OF CULTURE ON VARIOUS NUTRIENT MEDIAD. V. Ulshina0D. A. Kovalev1A. M. Zhirov2N. V. Zharinova3A. A. Khudoleev4O. I. Kogotkova5V. I. Efremenko6N. I. Evchenko7A. N. Kulichenko8Stavropol Institute for Plague ControlStavropol Institute for Plague ControlStavropol Institute for Plague ControlStavropol Institute for Plague ControlStavropol Institute for Plague ControlStavropol Institute for Plague ControlStavropol Institute for Plague ControlStavropol Institute for Plague ControlStavropol Institute for Plague ControlAim. Carry out comparative analysis using time-of-flight mass-spectrometry with matrix laser desorption/ionization (MALDI-TOF MS) of protein profiles of brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA), cultivated in various nutrient media: Albimi agar, brucellagar and erythrit-agar. Materials and methods. Vaccine strains: Brucella melitensis Rev-1 and Brucella abortus 19BA. Protein profiling in linear mode on Microflex «Bruker Daltonics» MALDI-TOF mass-spectrometer. Results. A number of characteristic features of brucella mass-spectra was detected: in particular, preservation of the total qualitative composition of protein profiles of cultures and significant differences in the intensity of separate peaks depending on the nutrient medium used. Conclusion. Based on the analysis of the data obtained, use of Albimi agar as the nutrient medium for preparation of brucella culture samples for mass-spectrometric analysis was shown to be optimal.https://microbiol.elpub.ru/jour/article/view/6mass-spectrometryprotein profilingbrucellosis causative agent |
| spellingShingle | D. V. Ulshina D. A. Kovalev A. M. Zhirov N. V. Zharinova A. A. Khudoleev O. I. Kogotkova V. I. Efremenko N. I. Evchenko A. N. Kulichenko FEATURES OF MASS-SPECTROMETRIC PROTEIN PROFILES OF STRAINS OF BRUCELLOSIS CAUSATIVE AGENT DURING PREPARATION OF CULTURE ON VARIOUS NUTRIENT MEDIA Журнал микробиологии, эпидемиологии и иммунобиологии mass-spectrometry protein profiling brucellosis causative agent |
| title | FEATURES OF MASS-SPECTROMETRIC PROTEIN PROFILES OF STRAINS OF BRUCELLOSIS CAUSATIVE AGENT DURING PREPARATION OF CULTURE ON VARIOUS NUTRIENT MEDIA |
| title_full | FEATURES OF MASS-SPECTROMETRIC PROTEIN PROFILES OF STRAINS OF BRUCELLOSIS CAUSATIVE AGENT DURING PREPARATION OF CULTURE ON VARIOUS NUTRIENT MEDIA |
| title_fullStr | FEATURES OF MASS-SPECTROMETRIC PROTEIN PROFILES OF STRAINS OF BRUCELLOSIS CAUSATIVE AGENT DURING PREPARATION OF CULTURE ON VARIOUS NUTRIENT MEDIA |
| title_full_unstemmed | FEATURES OF MASS-SPECTROMETRIC PROTEIN PROFILES OF STRAINS OF BRUCELLOSIS CAUSATIVE AGENT DURING PREPARATION OF CULTURE ON VARIOUS NUTRIENT MEDIA |
| title_short | FEATURES OF MASS-SPECTROMETRIC PROTEIN PROFILES OF STRAINS OF BRUCELLOSIS CAUSATIVE AGENT DURING PREPARATION OF CULTURE ON VARIOUS NUTRIENT MEDIA |
| title_sort | features of mass spectrometric protein profiles of strains of brucellosis causative agent during preparation of culture on various nutrient media |
| topic | mass-spectrometry protein profiling brucellosis causative agent |
| url | https://microbiol.elpub.ru/jour/article/view/6 |
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