Association between the Asp327Asn Polymorphism of Sex Hormone-Binding Globulin Gene and Prostate Cancer
Background: By binding SHBG hormone to sex hormones, in addition to carrying them in the blood, they regulate the amount of tissue availability. Since the genetic changes in the structure of globulin affect it’s binding to hormones, so in this study the effects of single nucleotide change...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Mazandaran University of Medical Sciences and Health Services
2016-07-01
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| Series: | Research in Molecular Medicine |
| Subjects: | |
| Online Access: | http://rmm.mazums.ac.ir/browse.php?a_code=A-10-629-2&slc_lang=en&sid=1 |
| _version_ | 1819063990648569856 |
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| author | Zahra Tahmaseni Fard Mandana Hasanzad Mohammad Reza Nowroozi Hanieh zham |
| author_facet | Zahra Tahmaseni Fard Mandana Hasanzad Mohammad Reza Nowroozi Hanieh zham |
| author_sort | Zahra Tahmaseni Fard |
| collection | DOAJ |
| description | Background: By binding SHBG hormone to sex hormones, in addition to carrying them in the blood, they regulate the amount of tissue availability. Since the genetic changes in the structure of globulin affect it’s binding to hormones, so in this study the effects of single nucleotide change in exon 8 or rs 6259 in the incidence of prostate cancer is evaluated.
Methods: The study population included 120 patients with prostate cancer and 120 control subjects. After collecting blood samples, DNA was extracted by salting out the method in order to determine the genotype of individuals by RFLP-PCR method. According to Hardy-Weinberg equilibrium genotypes and allele frequencies were calculated and a relationship between this variation and prostate cancer were evaluated and by using SPSS 23 software and the relationship between variations. The significance level was considered ≤0.05.
Results: Results indicated that homozygous mutant genotype AA 2.58 (p value= 0.007, OR: 2.58, CI95%: 1.52-4.38) and heterozygous AG 1.18 times (p-value =0.5, OR: 1.18, CI95%: 0.38-3.61) increase chance of getting prostate cancer in carriers. But homozygous of wild genotype GG have the protective role against prostate cancer (p-value =0.005, OR: 0.385, CI95%: 0.23-0.65).
Conclusion: Therefore, Asn allele is one of the main factors in prostate cancer so it can be used as the non-invasive and suitable marker for early detection in susceptible individuals. |
| first_indexed | 2024-12-21T15:23:27Z |
| format | Article |
| id | doaj.art-e0d4605411bf4ee99a1a5a6ed9e224dd |
| institution | Directory Open Access Journal |
| issn | 2322-1348 2322-133X |
| language | English |
| last_indexed | 2024-12-21T15:23:27Z |
| publishDate | 2016-07-01 |
| publisher | Mazandaran University of Medical Sciences and Health Services |
| record_format | Article |
| series | Research in Molecular Medicine |
| spelling | doaj.art-e0d4605411bf4ee99a1a5a6ed9e224dd2022-12-21T18:58:58ZengMazandaran University of Medical Sciences and Health ServicesResearch in Molecular Medicine2322-13482322-133X2016-07-01432934Association between the Asp327Asn Polymorphism of Sex Hormone-Binding Globulin Gene and Prostate CancerZahra Tahmaseni Fard0Mandana Hasanzad1Mohammad Reza Nowroozi2Hanieh zham3 Assistant Professor of cellular and Molecular Biology, Roudehen Branch, Islamic Azad University, Roudehen, IRAN. Medical Genomics Research Center, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran Uro Oncology Research Center, Tehran University of Medical Sciences, Tehran, Iran Department of pathology , Shohada Hospital, Shahid Beheshti University of Medical Sciences, Tehran, IRAN & Cancer Research Center, Shahid Beheshti University of Medical Sciences, Tehran, IRAN Background: By binding SHBG hormone to sex hormones, in addition to carrying them in the blood, they regulate the amount of tissue availability. Since the genetic changes in the structure of globulin affect it’s binding to hormones, so in this study the effects of single nucleotide change in exon 8 or rs 6259 in the incidence of prostate cancer is evaluated. Methods: The study population included 120 patients with prostate cancer and 120 control subjects. After collecting blood samples, DNA was extracted by salting out the method in order to determine the genotype of individuals by RFLP-PCR method. According to Hardy-Weinberg equilibrium genotypes and allele frequencies were calculated and a relationship between this variation and prostate cancer were evaluated and by using SPSS 23 software and the relationship between variations. The significance level was considered ≤0.05. Results: Results indicated that homozygous mutant genotype AA 2.58 (p value= 0.007, OR: 2.58, CI95%: 1.52-4.38) and heterozygous AG 1.18 times (p-value =0.5, OR: 1.18, CI95%: 0.38-3.61) increase chance of getting prostate cancer in carriers. But homozygous of wild genotype GG have the protective role against prostate cancer (p-value =0.005, OR: 0.385, CI95%: 0.23-0.65). Conclusion: Therefore, Asn allele is one of the main factors in prostate cancer so it can be used as the non-invasive and suitable marker for early detection in susceptible individuals.http://rmm.mazums.ac.ir/browse.php?a_code=A-10-629-2&slc_lang=en&sid=1Prostate cancer Sex hormone-binding globulin PCR-RFLP |
| spellingShingle | Zahra Tahmaseni Fard Mandana Hasanzad Mohammad Reza Nowroozi Hanieh zham Association between the Asp327Asn Polymorphism of Sex Hormone-Binding Globulin Gene and Prostate Cancer Research in Molecular Medicine Prostate cancer Sex hormone-binding globulin PCR-RFLP |
| title | Association between the Asp327Asn Polymorphism of Sex Hormone-Binding Globulin Gene and Prostate Cancer |
| title_full | Association between the Asp327Asn Polymorphism of Sex Hormone-Binding Globulin Gene and Prostate Cancer |
| title_fullStr | Association between the Asp327Asn Polymorphism of Sex Hormone-Binding Globulin Gene and Prostate Cancer |
| title_full_unstemmed | Association between the Asp327Asn Polymorphism of Sex Hormone-Binding Globulin Gene and Prostate Cancer |
| title_short | Association between the Asp327Asn Polymorphism of Sex Hormone-Binding Globulin Gene and Prostate Cancer |
| title_sort | association between the asp327asn polymorphism of sex hormone binding globulin gene and prostate cancer |
| topic | Prostate cancer Sex hormone-binding globulin PCR-RFLP |
| url | http://rmm.mazums.ac.ir/browse.php?a_code=A-10-629-2&slc_lang=en&sid=1 |
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