Analysis of the Expression of LSF Transcription Factor in the Regulation of Transcription and TSG101 during the Neoplastic Transformation of Endometrial Cells
Previous research indicates that carcinogenesis involves disrupting the functions of numerous genes, including factors involved in the regulation of transcription and cell proliferation. For these reasons, in endometrial carcinogenesis, we decided to investigate the expression of TSG101 (a suppresso...
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MDPI AG
2024-03-01
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| author | Rafał Ziemiński Aleksandra Stupak Maciej Kwiatek Tomasz Gęca Alicja Warowicka Karolina Hejne Anna Kwaśniewska Anna Goździcka-Józefiak Wojciech Kwaśniewski |
| author_facet | Rafał Ziemiński Aleksandra Stupak Maciej Kwiatek Tomasz Gęca Alicja Warowicka Karolina Hejne Anna Kwaśniewska Anna Goździcka-Józefiak Wojciech Kwaśniewski |
| author_sort | Rafał Ziemiński |
| collection | DOAJ |
| description | Previous research indicates that carcinogenesis involves disrupting the functions of numerous genes, including factors involved in the regulation of transcription and cell proliferation. For these reasons, in endometrial carcinogenesis, we decided to investigate the expression of TSG101 (a suppressor of tumor transformation) and LSF (a transcription factor involved in numerous cellular processes, such as cell cycle regulation, cell growth, development, and apoptosis). LSF may be involved in the regulation of TSG101 expression. The research material consisted of endometrial cancer samples from 60 patients. The control group consisted of normal endometrium samples donated by 60 women undergoing surgery for benign diseases of the female reproductive organs. The samples were subjected to immunohistochemical staining with antibodies specific to TSG101 and LSF. Specific antibodies were used to identify TSG101 and LSF in the examined histopathological preparations. An approximately 14-fold lower risk of endometrial cancer development was observed in patients with TSG expression in more than 75% of the assessed cells (4% vs. 36%; OR = 0.07; <i>p</i> = 0.0182). There was a four-fold lower risk of endometrial cancer development in patients with LSF expression in more than 50% of the assessed cells (32% vs. 64%; OR = 0.26; <i>p</i> = 0.0262). A more than three-fold lower risk of endometrial cancer development was observed in patients with LSF expression in more than 75% of the assessed cells (24% vs. 52%; OR = 0.29; <i>p</i> = 0.0454). Endometrial cancer was diagnosed in those with a lower level of TSG101 expression than in those with a cancer-free endometrium. Decreased expression of TSG101 may be a marker of endometrial cancer, and increased expression of LSF when diagnosed with endometrial cancer may indicate greater advancement of the disease. These markers might be used as diagnostic and prognostic markers—however, there is a lack of a correlation between them. |
| first_indexed | 2024-04-24T10:48:20Z |
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| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-04-24T10:48:20Z |
| publishDate | 2024-03-01 |
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| spelling | doaj.art-e0d8732251aa42d1851f9f2f9aad455c2024-04-12T13:16:26ZengMDPI AGCells2073-44092024-03-0113758010.3390/cells13070580Analysis of the Expression of LSF Transcription Factor in the Regulation of Transcription and TSG101 during the Neoplastic Transformation of Endometrial CellsRafał Ziemiński0Aleksandra Stupak1Maciej Kwiatek2Tomasz Gęca3Alicja Warowicka4Karolina Hejne5Anna Kwaśniewska6Anna Goździcka-Józefiak7Wojciech Kwaśniewski8Department of Obstetrics and Pathology of Pregnancy, Medical University of Lublin, 20-059 Lublin, PolandDepartment of Obstetrics and Pathology of Pregnancy, Medical University of Lublin, 20-059 Lublin, PolandDepartment of Obstetrics and Pathology of Pregnancy, Medical University of Lublin, 20-059 Lublin, PolandDepartment of Obstetrics and Pathology of Pregnancy, Medical University of Lublin, 20-059 Lublin, PolandDepartment of Molecular Virology, Institute of Experimental Biology, Adam Mickiewicz University in Poznan, 61-712 Poznań, PolandDepartment of Pathomorphology and Forensic Medicine, School of Medicine, Collegium Medicum, University of Warmia and Mazury, 11-082 Olsztyn, PolandDepartment of Obstetrics and Pathology of Pregnancy, Medical University of Lublin, 20-059 Lublin, PolandDepartment of Molecular Virology, Institute of Experimental Biology, Adam Mickiewicz University in Poznan, 61-712 Poznań, PolandDepartment of Gynecology Oncology and Gynecology, Medical University of Lublin, 20-059 Lublin, PolandPrevious research indicates that carcinogenesis involves disrupting the functions of numerous genes, including factors involved in the regulation of transcription and cell proliferation. For these reasons, in endometrial carcinogenesis, we decided to investigate the expression of TSG101 (a suppressor of tumor transformation) and LSF (a transcription factor involved in numerous cellular processes, such as cell cycle regulation, cell growth, development, and apoptosis). LSF may be involved in the regulation of TSG101 expression. The research material consisted of endometrial cancer samples from 60 patients. The control group consisted of normal endometrium samples donated by 60 women undergoing surgery for benign diseases of the female reproductive organs. The samples were subjected to immunohistochemical staining with antibodies specific to TSG101 and LSF. Specific antibodies were used to identify TSG101 and LSF in the examined histopathological preparations. An approximately 14-fold lower risk of endometrial cancer development was observed in patients with TSG expression in more than 75% of the assessed cells (4% vs. 36%; OR = 0.07; <i>p</i> = 0.0182). There was a four-fold lower risk of endometrial cancer development in patients with LSF expression in more than 50% of the assessed cells (32% vs. 64%; OR = 0.26; <i>p</i> = 0.0262). A more than three-fold lower risk of endometrial cancer development was observed in patients with LSF expression in more than 75% of the assessed cells (24% vs. 52%; OR = 0.29; <i>p</i> = 0.0454). Endometrial cancer was diagnosed in those with a lower level of TSG101 expression than in those with a cancer-free endometrium. Decreased expression of TSG101 may be a marker of endometrial cancer, and increased expression of LSF when diagnosed with endometrial cancer may indicate greater advancement of the disease. These markers might be used as diagnostic and prognostic markers—however, there is a lack of a correlation between them.https://www.mdpi.com/2073-4409/13/7/580carcinogenesisLSF transcription factorTSG101endometrial cancer |
| spellingShingle | Rafał Ziemiński Aleksandra Stupak Maciej Kwiatek Tomasz Gęca Alicja Warowicka Karolina Hejne Anna Kwaśniewska Anna Goździcka-Józefiak Wojciech Kwaśniewski Analysis of the Expression of LSF Transcription Factor in the Regulation of Transcription and TSG101 during the Neoplastic Transformation of Endometrial Cells Cells carcinogenesis LSF transcription factor TSG101 endometrial cancer |
| title | Analysis of the Expression of LSF Transcription Factor in the Regulation of Transcription and TSG101 during the Neoplastic Transformation of Endometrial Cells |
| title_full | Analysis of the Expression of LSF Transcription Factor in the Regulation of Transcription and TSG101 during the Neoplastic Transformation of Endometrial Cells |
| title_fullStr | Analysis of the Expression of LSF Transcription Factor in the Regulation of Transcription and TSG101 during the Neoplastic Transformation of Endometrial Cells |
| title_full_unstemmed | Analysis of the Expression of LSF Transcription Factor in the Regulation of Transcription and TSG101 during the Neoplastic Transformation of Endometrial Cells |
| title_short | Analysis of the Expression of LSF Transcription Factor in the Regulation of Transcription and TSG101 during the Neoplastic Transformation of Endometrial Cells |
| title_sort | analysis of the expression of lsf transcription factor in the regulation of transcription and tsg101 during the neoplastic transformation of endometrial cells |
| topic | carcinogenesis LSF transcription factor TSG101 endometrial cancer |
| url | https://www.mdpi.com/2073-4409/13/7/580 |
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